Table 3.

Recommendations for Compositional Analysis of Atherosclerotic Lesions

1. Validation of authenticity of antibody reactivity needs to be provided.
2. Because atherosclerotic lesions are heterogeneous, selection criteria for tissue section analysis need to be stated. Recommendations are to determine multiple sections per lesion.
3. Quantitation of immunostaining must include complete details on the mode of data capture.
4. Compositional data should be referred to by objective descriptors and not by inferential descriptors (eg, vulnerable or unstable).
5. Considering phenotypic plasticity, simultaneous use of multiple cell type–specific markers enhances the accuracy of data acquisition.