Table 4.

Checklist of Methodological and Reporting Aspects for Articles Submitted to Circulation Research That Report Molecular and/or Cellular Studies In Vitro

Methodological and Reporting AspectsDescription of Procedures
Conduct of the study◻ Technical replicates, whenever applicable, such as RNA-sequencing and ChIP experiments, are described.
◻ Sufficient information about sample collection is provided to distinguish between
independent biological data points and technical replicates.
◻ Specific characteristics of each antibody used, such as source, host, immunoglobulin fragment, titer, catalog number, and how the antibody was validated, are described.
◻ Specific characteristics of each cell line used, such as source, authentication, and passage number, are described.
◻ Any data processing and conversions are described.
Data reporting◻ In Western blots, all experimental and control groups are included in the same blot.
◻ Molecular size markers are included in all blots.
◻ Immunofluorescence imaging is processed similarly in the experimental and control groups.
◻ A control secondary antibody or IgG is included in immunofluorescence imaging.
◻ Scale bars have been added to all micrographs.
◻ All units of measurements are specified.
◻ To the extent possible, data are reported as dot plots as opposed to bar graphs, especially for small sample size groups.
◻ In the online supplemental material, methods are described in sufficient detail to enable full replication of the study.
Statistical methods◻ The statistical methods used for each data set are described.
◻ For each statistical test, the effect size with its standard error, 95% confidence interval (for important comparisons), and P value is presented.
◻ Central tendency and dispersion of the data are examined, particularly for small data sets.
◻ Nonparametric tests are used for data that are not normally distributed.
◻ 2-sided P values are used.
◻ In studies that are not exploratory in nature, corrections for multiple hypotheses testing and multiple comparisons are performed.
  • The purpose of this checklist is to provide important metrics for the conduct and reporting of molecular and cellular studies in vitro. The criteria listed herewith are intended to (i) clearly communicate the desired standards of rigor to authors and investigators and (ii) facilitate the journal’s overall mission of promoting robust, rigorous, and reproducible scientific inquiry and experimentation. The information requested herein is meant to be used by reviewers and editors as an aid in assessing the value of the work, not as a rigid criterion for accepting or rejecting articles; thus, decisions on acceptance or rejection will be made on a case-by-case basis depending on the individual content and message of each submitted article. In addition, we recognize that certain standards outlined above may not apply to exploratory, hypothesis-generating, or mechanistic studies. In these cases, we encourage the authors to address the criteria outlined here and describe why adherence to particular standards was not practical or meaningful. We ask authors to use online supplements for providing detailed experimental protocols and communicating any secondary results not reported in the main article.