Cardiac c-Kit Biology Revealed by Inducible Transgenesis

Abstract
Rationale: Biological significance of c-Kit as a cardiac stem cell marker and role(s) of c-Kit+ cells in myocardial development or response to pathologic injury remain unresolved due to varied and discrepant findings. Alternative experimental models are required to contextualize and reconcile discordant published observations of cardiac c-Kit myocardial biology and provide meaningful insights regarding clinical relevance of c-Kit signaling for translational cell therapy.
Objective: Demonstration of c-Kit myocardial biology through combined studies of both human and murine cardiac cells. Advancing understanding of c-Kit myocardial biology through creation and characterization of a novel, inducible transgenic c-Kit reporter mouse model that overcomes limitations inherent to knock-in reporter models, providing perspective to reconcile disparate viewpoints on c-Kit biology in the myocardium.
Methods and Results: In vitro studies confirm a critical role for c-Kit signaling in both cardiomyocytes and cardiac stem cells. Activation of c-Kit receptor promotes cell survival and proliferation in stem cells and cardiomyocytes of either human or murine origin. For creation of the mouse model, the cloned mouse c-Kit promoter drives Histone2B-EGFP (H2BEGFP) expression in a doxycycline inducible transgenic reporter line. The combination of c-Kit transgenesis coupled to H2BEGFP readout provides sensitive, specific, inducible, and persistent tracking of c-Kit promoter activation. Tagging efficiency for EGFP+/c-Kit+ cells is similar between our transgenic versus a c-Kit knock-in mouse line, but frequency of c-Kit+ cells in cardiac tissue from the knock-in model is 55% lower than our transgenic line. The c-Kit transgenic reporter model reveals intimate association of c-Kit expression with adult myocardial biology. Both cardiac stem cells and a subpopulation of cardiomyocytes express c-Kit in uninjured adult heart, upregulating c-Kit expression in response to pathologic stress.
Conclusions: c-Kit myocardial biology is more complex and varied than previously appreciated or documented, demonstrating validity in multiple points of coexisting yet heretofore seemingly irreconcilable published findings.
- Received August 2, 2017.
- Revision received March 24, 2018.
- Accepted April 9, 2018.
American Heart Association Professional?
Log in using your username and password
Pay Per Article - You may access this article (from the computer you are currently using) for 1 day for US$35.00
Regain Access - You can regain access to a recent Pay per Article purchase if your access period has not yet expired.
Current Issue
Article Tools
- Cardiac c-Kit Biology Revealed by Inducible TransgenesisNatalie A Gude, Fareheh Firouzi, Kathleen M Broughton, Kelli Ilves, Kristine P Nguyen, Christina R Payne, Veronica Sacchi, Megan M Monsanto, Alexandria R Casillas, Farid G Khalafalla, Bingyan J Wang, David Ebeid, Roberto Alvarez, Walter P Dembitsky, Barbara A Bailey, Jop H van Berlo and Mark A SussmanCirculation Research. 2018;CIRCRESAHA.117.311828, originally published April 10, 2018https://doi.org/10.1161/CIRCRESAHA.117.311828
Citation Manager Formats
Share this Article
- Cardiac c-Kit Biology Revealed by Inducible TransgenesisNatalie A Gude, Fareheh Firouzi, Kathleen M Broughton, Kelli Ilves, Kristine P Nguyen, Christina R Payne, Veronica Sacchi, Megan M Monsanto, Alexandria R Casillas, Farid G Khalafalla, Bingyan J Wang, David Ebeid, Roberto Alvarez, Walter P Dembitsky, Barbara A Bailey, Jop H van Berlo and Mark A SussmanCirculation Research. 2018;CIRCRESAHA.117.311828, originally published April 10, 2018https://doi.org/10.1161/CIRCRESAHA.117.311828