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Original Research

Cardiac c-Kit Biology Revealed by Inducible Transgenesis

Natalie A Gude, Fareheh Firouzi, Kathleen M Broughton, Kelli Ilves, Kristine P Nguyen, Christina R Payne, Veronica Sacchi, Megan M Monsanto, Alexandria R Casillas, Farid G Khalafalla, Bingyan J Wang, David Ebeid, Roberto Alvarez, Walter P Dembitsky, Barbara A Bailey, Jop H van Berlo, Mark A Sussman
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https://doi.org/10.1161/CIRCRESAHA.117.311828
Circulation Research. 2018;CIRCRESAHA.117.311828
Originally published April 10, 2018
Natalie A Gude
Biology, San Diego State University
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Fareheh Firouzi
San Diego Heart Research Institute, San Diego State University
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Kathleen M Broughton
San Diego Heart Research Institute, San Diego State University
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Kelli Ilves
San Diego Heart Research Institute, San Diego State University
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Kristine P Nguyen
San Diego Heart Research Institute, San Diego State University
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Christina R Payne
San Diego Heart Research Institute, San Diego State University
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Veronica Sacchi
Biology, San Diego State University
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Megan M Monsanto
San Diego Heart Research Institute, San Diego State University
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Alexandria R Casillas
San Diego Heart Research Institute, San Diego State University
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Farid G Khalafalla
San Diego Heart Research Institute, San Diego State University
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Bingyan J Wang
San Diego Heart Research Institute, San Diego State University
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David Ebeid
San Diego Heart Research Institute, San Diego State University
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Roberto Alvarez
San Diego Heart Research Institute, San Diego State University
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Walter P Dembitsky
Sharp Memorial Hospital
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Barbara A Bailey
Mathematics and Statistics, San Diego State University
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Jop H van Berlo
Medicine, University of Minnesota
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Mark A Sussman
San Diego Heart Research Institute, San Diego State University
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  • For correspondence: heartman4ever@icloud.com
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Abstract

Rationale: Biological significance of c-Kit as a cardiac stem cell marker and role(s) of c-Kit+ cells in myocardial development or response to pathologic injury remain unresolved due to varied and discrepant findings. Alternative experimental models are required to contextualize and reconcile discordant published observations of cardiac c-Kit myocardial biology and provide meaningful insights regarding clinical relevance of c-Kit signaling for translational cell therapy.

Objective: Demonstration of c-Kit myocardial biology through combined studies of both human and murine cardiac cells. Advancing understanding of c-Kit myocardial biology through creation and characterization of a novel, inducible transgenic c-Kit reporter mouse model that overcomes limitations inherent to knock-in reporter models, providing perspective to reconcile disparate viewpoints on c-Kit biology in the myocardium.

Methods and Results: In vitro studies confirm a critical role for c-Kit signaling in both cardiomyocytes and cardiac stem cells. Activation of c-Kit receptor promotes cell survival and proliferation in stem cells and cardiomyocytes of either human or murine origin. For creation of the mouse model, the cloned mouse c-Kit promoter drives Histone2B-EGFP (H2BEGFP) expression in a doxycycline inducible transgenic reporter line. The combination of c-Kit transgenesis coupled to H2BEGFP readout provides sensitive, specific, inducible, and persistent tracking of c-Kit promoter activation. Tagging efficiency for EGFP+/c-Kit+ cells is similar between our transgenic versus a c-Kit knock-in mouse line, but frequency of c-Kit+ cells in cardiac tissue from the knock-in model is 55% lower than our transgenic line. The c-Kit transgenic reporter model reveals intimate association of c-Kit expression with adult myocardial biology. Both cardiac stem cells and a subpopulation of cardiomyocytes express c-Kit in uninjured adult heart, upregulating c-Kit expression in response to pathologic stress.

Conclusions: c-Kit myocardial biology is more complex and varied than previously appreciated or documented, demonstrating validity in multiple points of coexisting yet heretofore seemingly irreconcilable published findings.

  • c-Kit
  • molecular
  • cardiac
  • stem cell
  • cardiomyocyte
  • myocardium
  • signal transduction
  • Received August 2, 2017.
  • Revision received March 24, 2018.
  • Accepted April 9, 2018.

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    Cardiac c-Kit Biology Revealed by Inducible Transgenesis
    Natalie A Gude, Fareheh Firouzi, Kathleen M Broughton, Kelli Ilves, Kristine P Nguyen, Christina R Payne, Veronica Sacchi, Megan M Monsanto, Alexandria R Casillas, Farid G Khalafalla, Bingyan J Wang, David Ebeid, Roberto Alvarez, Walter P Dembitsky, Barbara A Bailey, Jop H van Berlo and Mark A Sussman
    Circulation Research. 2018;CIRCRESAHA.117.311828, originally published April 10, 2018
    https://doi.org/10.1161/CIRCRESAHA.117.311828

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    Cardiac c-Kit Biology Revealed by Inducible Transgenesis
    Natalie A Gude, Fareheh Firouzi, Kathleen M Broughton, Kelli Ilves, Kristine P Nguyen, Christina R Payne, Veronica Sacchi, Megan M Monsanto, Alexandria R Casillas, Farid G Khalafalla, Bingyan J Wang, David Ebeid, Roberto Alvarez, Walter P Dembitsky, Barbara A Bailey, Jop H van Berlo and Mark A Sussman
    Circulation Research. 2018;CIRCRESAHA.117.311828, originally published April 10, 2018
    https://doi.org/10.1161/CIRCRESAHA.117.311828
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  • Genetics
    • Genetically Altered and Transgenic Models
  • Basic, Translational, and Clinical Research
    • Myocardial Biology
    • Stem Cells
    • Cell Signaling/Signal Transduction
    • Basic Science Research

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