Regulated Transgene Expression in Vascular Smooth Muscle
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To the Editor:
We are writing in reference to 2 articles published in Circulation Research, both of which report use of transgenic mice with smooth muscle cell–specific expression of the tetracycline-regulated transactivator (tTA) to activate conditional tTA-regulated alleles in the vasculature of transgenic mice.1,2 The purpose of this letter is to report our experience with these mice and to inform the readership that, in our hands, these mice show no evidence of either (1) an ability to transactivate conditional alleles or (2) expression of the tTA in vascular tissue.
Generation of mice in which a fragment of the murine SM22α promoter was used to achieve smooth muscle–specific expression of the tTA was first reported in abstract form in 1997 by Husain et al.3 In 1998, experimental results obtained with these mice appeared in a figure contributed by Dr Husain to a review article in Circulation …