Histochemical Studies in Atherogenesis
Human Cerebral Arteries
Histochemieal studies of grossly normal and early lesions of cerebral arteries of 75 iudividuals ranging from fetuses to 70 years of age were made, utilizing newer histochemieal techniques. The following conclusions were made: There is no apparent relationship between acid mucopolysaccharides and lipids in respect to staining intensity and/or distribution in any of the age groups. In older juveniles and adults, lipid material was consistently observed intimately associated with the internal elastic membrane and other reduplicated elastic fibers, even in the absence of gross lesions. Fragmentation, fraying, and/or reduplication of the internal elastic membrane were absent in the fetuses, infants, and young juveniles. There was no apparent association between lipid and these elastic changes. In fetuses, infants, and young juveniles there was an accumulation of acid mucopolysaccharides hydrolyzable by testicular hyaluronidase, principally in the area of the proximal media, suggesting that the polysaccharide material was hyaluronic acid and/or chondroitin sulfate A and/or chondroitin sulfate C. In the adults, there was an accumulation in acid mucopolysaccharides resistant to hydrolysis by testicular hyaluronidase in the proximal media and in the intima. This accumulation in acid mucopolysaccharides corresponded to the areas of collagen increase. It was further suggested that these polysaccharides were chondroitin sulfate B and/or heparitin sulfate and/or hepa-rin. The significance of these findings was discussed.
- Received February 18, 1961.
- © 1961 American Heart Association, Inc.