Influence of Cholesterol Feeding on Phospholipid Metabolism of Connective Tissue
Subcutaneous injection of carrageenan, a sulfated polygalactose extracted from the red algae Chondrus crispus, stimulated the production of fibrous connective tissue in the rabbit. Animals fed cholesterol for 3 and 5 weeks had significantly higher phospholipid concentrations in this connective tissue than did control rabbits, but no alteration in phospholipid specific activity. These results are similar to those obtained in preliminary studies of connective tissue cultured by the sponge implantation technic.
Significant increases occurred in cephalin, lecithin, and sphingomyelin concentrations in tissue proliferated after cearrageenan injection. The cholesterol content of connective tissue was increased 7- to 8-fold.
Histologically, the tissue taken after 3 weeks of cholesterol feeding could not be distinguished from that of control animals. At 5 weeks, connective tissue from cholesterol-fed rabbits contained mature fat cells and macrophages resembling foam cells, in addition to the fibroblasts and collagen present in control tissue. Development of collagenous fibers and alterations in lipids were not found to be directly related. It is suggested that the elaboration of collagen and the accumulation of phospholipid, both of which occur in experimental atheromatosis, represent independent processes of connective tissue cells.
- Received February 4, 1960.
- © 1960 American Heart Association, Inc.