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Validity and Limitations of Rat Mesoappendix Bioassay Studies
BENJAMIN W. ZWEIFACH, DELILAH B. METZ
https://doi.org/10.1161/01.RES.3.2.121
Circulation Research. 1955;3:121-132
Originally published March 1, 1955Jump to

Abstract
Bioassay data obtained by the rat mesoappendix method lend themselves poorly to statistical representation. Sufficient reproducibility for quantitative estimation of the titer of vasoactive materials was achieved only with crystalline substances or purified biological extracts. Neither the duration of the displacement of epinephrine reactivity nor the magnitude of the shift in effective constrictor doses of epinephrine provided a satisfactory measure of vasoactivity in blood specimens. A prozone phenomenon, with agents such as Pitressin, histamine and ferritin was encountered with potentiating effects being elicited in high dose ranges and inhibitory effects in low dose ranges.
- Received August 19, 1954.
- © 1955 American Heart Association, Inc.
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- Validity and Limitations of Rat Mesoappendix Bioassay StudiesBENJAMIN W. ZWEIFACH and DELILAH B. METZCirculation Research. 1955;3:121-132, originally published March 1, 1955https://doi.org/10.1161/01.RES.3.2.121
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