Prostaglandin-Like Substances Appearing in Canine Renal Venous Blood During Renal Ischemia
Their Partial Characterization By Pharmacologic And Chromatographic Procedures
Renal prostaglandins (PCs) might mediate an antihypertensive function of the kidney. The blood-superfused organ technique possesses the sensitivity (threshold < 0.4 ng/ml blood) and specificity required for identification of PGs in blood. Induction of unilateral renal ischemia in 14 chloraloseanesthetized dogs reduced renal blood flows from a mean value of 257 to 109 ml/min on the ischemic side and from 250 to 209 ml/min on the contralateral side. Concomitantly, PG-like substances were detected by assay organs in the venous blood of ischemic (13 experiments) and contralateral (11 experiments) kidneys. In one experiment, in a spontaneously hypertensive dog, PGs were not detected during renal ischemia.
Renal venous blood and renal medullary tissue were extracted for acidic lipids and assayed for PG-like substances. Extracts of venous blood collected during renal ischemia and extracts of renal medulla yielded substances with biological activity indistinguishable from PG-like substances or PG standards. Chromatographic characterization of PG-like substances suggests that they are predominantly a mixture of PGE2 and PGF2α.
- antihypertensive function of kidney
- angiotensin II
- blood-superfused organ technique
- renal medulla
- acidic lipids
- renal prostaglandins
- renal blood flows
- renal vascular hypertension
- Received March 24, 1970.
- Accepted September 4, 1970.
- © 1970 American Heart Association, Inc.