Tissue Localization and Plasma Activity With Special Reference to Cardiovascular Disease and Lupus Erythematosus
Two experimental approaches were made to gain insight into the physiological significance of cardioglobulin. The first was to determine whether Cardioglobulin was localized to a particular organ or tissue, since earlier studies had suggested that the system might function as a regulator of myocardial contractility. By fluorescence localization of antibodies to rat cardioglobulin-C, cardioglobulin-C was found not only on the surface of heart muscle cells, but in other extracellular locations which, broadly speaking, were either cell surfaces or basement membrane. The second phase of the study was measurement of plasma activity of cardioglobulin-A, -B, and -C in human diseases. The previously discovered increased overall activity in patients with elevated left ventricular systolic pressure was due to increased cardioglobulin-A. Decreased activity in patients with idiopathic myocardial failure was due to decreased cardioglobulin-C. Patients with advanced cirrhosis and evidence of impaired protein production also had low cardioglobulin-C. Low plasma cardioglobulin was found in 22 of 24 patients with systemic lupus erythematosus. Cardioglobulin was normal in all of 17 patients with other connective tissue diseases. The new tissue and clinical findings may provide a clue in the search for the functional significance of this recently discovered protein system.
- plasma proteins
- idiopathic heart failure
- connective tissue diseases
- immune fluorescence
- © 1968 American Heart Association, Inc.