Incorporation of Sulfate-S35 and Glucosamine-1-C14 into Heparin During Perfusion of Isolated Rat Liver
Sulfate-S35 and glucosamine-1-C14 are incorporated into heparin which has been added to the blood perfusing an isolated, surviving rat liver. Although both labels are firmly bound to heparin, such uptake does not necessarily indicate synthesis of the polymer.
Despite the fact that the perfused heparin has undergone some moderate depolymerization, its biological activity remains unchanged.
The presence in the bile of material containing hexuronic acid and hexosamine but deprived of anticoagulant activity suggests that products derived from heparin breakdown may be eliminated in the bile.
- Received May 8, 1964.
- © 1964 American Heart Association, Inc.