Abstract 54: Effect of Salubrinal and PP1 on Cardiomyocytes Protection After Myocardial Infarction in Rats
Objective: salubrinal, an inhibitor of ER-stress, has potent effect on cell injury. In this study, we investigated the effect of salubrinal and its dervative-PP1 on cell protection aganinst myocardial infarction ( MI).
Methods: Male wistar rats were randomly divided into 5 groups:i) sham-operated; ii) vehicle (MI+ DMSO); iii) MI + salubrinal; iv) MI + PP1; v) MI + metoprolol. After 24 hours treatment, heart tissue was havested and stained by 2,3,5-triphenyltetrazolium chloride (TTC). Serum was havested for the detection of CK-MB, cTNT, CRP. The changes in histomophology were observed using hematoxylin and eosin (HE) staining. The expression of mRNA and protein involved in apoptosis, autophagy, ER stress was analyzed using real-time reverse transcription-polymerase chain reaction and western blotting, respectively.
Results: Salubrinal and PP1 decreased the infarction area and the level of CK-MB, cTNT, CRP. The mechanism analysis demonsted that salubrinal and PP1 have a cell protection through a ER-stress related pathway, showing upregualtion of PERK, eIF2α, ATF4, GRP78, GADD34, p62 , LC3IIand down regualtion of chop, caspase12 as detected by real-time PCR and western blotting.
Conclusions: The current study suggested that salubrinal and PP1 have a cell protection effect involed in ER stress related autophay and apoptosis.This may implicated that salubrinal and PP1 may be a potent compound for the treatment of MI.
Author Disclosures: C. Liu: None. Y. He: None. K. He: None.
- © 2015 by American Heart Association, Inc.