Abstract 300: A Novel Targeted Angiogenesis Technique Using Magnetic Nanoparticles
Aims: To develop a novel technique for angiogenesis which could be potentially useful for therapeutic purposes using magnetic nanoparticles.
Methods: Magnetic nanoparticles were synthesized and were conjugated with vascular endothelial growth factor. The particles were tested in tissue culture microfluidic chips for angiogenesis. Four layers were generated for the experiment respectively - the nutrient layer, hydrogel layer, HUVEC spheroids and another hydrogel layer in the bottom. The particles were inserted in a layer of collagen hydrogel and were guided to desired location using a magnet. The extent and the direction of angiogenesis were studied using 3D confocal microscopy.
Results: Angiogenesis was observed compared to the controls when nanoparticles were interfaced between the nutrient and HUVEC layer. When the nanoparticles was placed below the HUVEC spheroids angiogenesis occurred in the basal layer predominantly, which showed the effect of the nanoparticles with growth factor on angiogenesis more than the nutrients. In another experiment in microfluidic chip, the nanoparticles were placed in nutrient layer and HUVEC cell monolayer was placed underneath the nutrient layer. The nanoparticles was observed to cross endothelial cells and reach lower hydrogel layer, which was observed by Z stack confocal microscopy.
Conclusion: Angiogenesis happen by the effect of magnetic nanoparticles conjugated with growth factors. These nanoparticles can be controlled with a magnet. Also, these nanoparticles have potentials to cross endothelium.
Author Disclosures: M. Arokiaraj: None.
- © 2015 by American Heart Association, Inc.