Abstract 109: Characterization of Secretome From C-kit+ Cells Derived From Neonate and Adult Heart Patients
Background: Despite the regenerative benefit reported in recent human clinical trials (SCIPIO trial) using human cardiac stem cells (CSCs) (identified by ckit+CD45-/lin-), low retention and differentiation of transplanted hCSCs into cardiomyocytes are insufficient to explain the myocardial regeneration. This discrepancy has led to the paracrine hypothesis of stem cell action that indicates communication between newly transplanted stem cells and reparative native cells, essentially jumpstarting the repair process.
Methods: We derived CSCs from the biopsies of right atrial appendage (RAA) of neonatal human subjects (nCSCs, age < 2 months) and from adult human subjects (aCSCs, age >50 years). CSCs were grown upto 90% confluency and incubated with basal medium (Ham’s/F12) for 72 hours. Conditioned medium (CM) was collected and further analyzed.
Results: The expression levels of various key cytokines were analyzed using ELISA and data showed nCSCs secrete higher levels of the angiogenic cytokines like SDF1, VEGF-A, ANG, SCF, PDGB, and FGF2 compared to over 20 other cytokines we examined as compared to aCSCs. CM derived from nCSCs showed more angiogeneic potential as compared to CM derived from aCSCs as analyzed by HuVEC tube assay formation. We also did a comparative analysis of CM from nCSCs and aCSCs using mass spectrometry. Molecular distribution of lead proteins revealed that the yhCSCs has more angiogenic, anti-inflammatory and proliferative proteins while ahCSCs have more of apoptotic proteins
Conclusion: These data provide preliminary evidence that there is a difference in the cytokine secretion by the nCSCs as compared to aCSCs and implicates highly expressed cytokines as the key to myocardial recovery.
Author Disclosures: S. Sharma: None. R. Mishra: None. Y.A. Goo: None. B.P. Wehman: None. Y. Guo: None. S.R. Datla: None. K. Balachandran: None. O.T. Siddiqi: None. S. Kaushal: None.
- © 2015 by American Heart Association, Inc.