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Molecular Medicine

Deletion of Yes-Associated Protein (YAP) Specifically in Cardiac and Vascular Smooth Muscle Cells Reveals a Crucial Role for YAP in Mouse Cardiovascular DevelopmentNovelty and Significance

Yong Wang, Guoqing Hu, Fang Liu, Xiaobo Wang, Mingfu Wu, John J. Schwarz, Jiliang Zhou
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https://doi.org/10.1161/CIRCRESAHA.114.303411
Circulation Research. 2014;114:957-965
Originally published March 13, 2014
Yong Wang
From the Department of Pharmacology and Toxicology, Medical College of Georgia, Georgia Regents University, Augusta (Y.W., G.H., F.L., J.Z.); and Center for Cardiovascular Sciences, Albany Medical College, NY (X.W., M.W., J.J.S.).
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Guoqing Hu
From the Department of Pharmacology and Toxicology, Medical College of Georgia, Georgia Regents University, Augusta (Y.W., G.H., F.L., J.Z.); and Center for Cardiovascular Sciences, Albany Medical College, NY (X.W., M.W., J.J.S.).
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Fang Liu
From the Department of Pharmacology and Toxicology, Medical College of Georgia, Georgia Regents University, Augusta (Y.W., G.H., F.L., J.Z.); and Center for Cardiovascular Sciences, Albany Medical College, NY (X.W., M.W., J.J.S.).
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Xiaobo Wang
From the Department of Pharmacology and Toxicology, Medical College of Georgia, Georgia Regents University, Augusta (Y.W., G.H., F.L., J.Z.); and Center for Cardiovascular Sciences, Albany Medical College, NY (X.W., M.W., J.J.S.).
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Mingfu Wu
From the Department of Pharmacology and Toxicology, Medical College of Georgia, Georgia Regents University, Augusta (Y.W., G.H., F.L., J.Z.); and Center for Cardiovascular Sciences, Albany Medical College, NY (X.W., M.W., J.J.S.).
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John J. Schwarz
From the Department of Pharmacology and Toxicology, Medical College of Georgia, Georgia Regents University, Augusta (Y.W., G.H., F.L., J.Z.); and Center for Cardiovascular Sciences, Albany Medical College, NY (X.W., M.W., J.J.S.).
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Jiliang Zhou
From the Department of Pharmacology and Toxicology, Medical College of Georgia, Georgia Regents University, Augusta (Y.W., G.H., F.L., J.Z.); and Center for Cardiovascular Sciences, Albany Medical College, NY (X.W., M.W., J.J.S.).
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Abstract

Rationale: Our previous study has shown that yes-associated protein (YAP) plays a crucial role in the phenotypic modulation of vascular smooth muscle cells (SMCs) in response to arterial injury. However, the role of YAP in vascular SMC development is unknown.

Objective: The goal of this study was to investigate the functional role of YAP in cardiovascular development in mice and determine the mechanisms underlying YAP’s actions.

Methods and Results: YAP was deleted in cardiomyocytes and vascular SMCs by crossing YAP flox mice with SM22α-Cre transgenic mice. Cardiac/SMC-specific deletion of YAP directed by SM22α-Cre resulted in perinatal lethality in mice because of profound cardiac defects including hypoplastic myocardium, membranous ventricular septal defect, and double outlet right ventricle. The cardiac/SMC-specific YAP knockout mice also displayed severe vascular abnormalities including hypoplastic arterial wall, short/absent brachiocephalic artery, and retroesophageal right subclavian artery. Deletion of YAP in mouse vascular SMCs induced expression of a subset of cell cycle arrest genes including G-protein–coupled receptor 132 (Gpr132). Silencing Gpr132 promoted SMC proliferation, whereas overexpression of Gpr132 attenuated SMC growth by arresting cell cycle in G0/G1 phase, suggesting that ablation of YAP-induced impairment of SMC proliferation was mediated, at least in part, by induction of Gpr132 expression. Mechanistically, YAP recruited the epigenetic repressor histone deacetylase-4 to suppress Gpr132 gene expression via a muscle CAT element in the Gpr132 gene.

Conclusions: YAP plays a critical role in cardiac/SMC proliferation during cardiovascular development by epigenetically regulating expression of a set of cell cycle suppressors.

  • abnormalities
  • blood supply
  • developmental biology
  • muscle, smooth
  • muscle, smooth, vascular
  • myocardium
  • transcription factors

Introduction

Vascular wall assembly is critical in the process of the cardiovascular development. This developmental process requires a coordination of multiple cell types including endothelial and smooth muscle cells (SMCs). After establishment of a nascent capillary-like vascular network by endothelial cells, vascular SMC progenitors begin to invest the vessel wall through migration and proliferation. Meanwhile, vascular SMCs acquire a unique array of differentiated contractile markers such as SM α-actin, calponin, hydrogen peroxide–inducible clone 5,1 SM myosin heavy chain, 130-kDa myosin light chain kinase, and SM22α.2 Defects of molecular pathways that control the migration, proliferation, and differentiation of vascular SMCs will produce malformations of blood vessels and aberrant activation of signaling pathways in response to vascular diseases. Therefore, a better understanding of the mechanisms underlying SMC development is essential to not only advance our knowledge with vascular wall biology but also for identifying genes whose defects cause congenital vascular defects or vascular wall diseases in humans.2,3

In This Issue, see p 929

The Hippo pathway is crucial for controlling organ size and tumorigenesis.4–6 The mammalian core components of Hippo pathway include mammalian sterile 20-like 1 or 2, large tumor suppressor 1 or 2, signaling downstream effector yes-associated protein (YAP), and YAP binding partner TEA domain family member (TEAD) family proteins. On stimulation, mammalian sterile 20-like 1 or 2 phosphorylates downstream large tumor suppressor 1 or 2 that subsequently phosphorylates YAP and retains YAP in the cytoplasm. Unphosphorylated YAP translocates into the nucleus where it binds TEAD family proteins to induce genes that promote cell growth and oncogenic transformation.7 Recently, several studies showed that the Hippo–YAP pathway plays a critical role in mouse cardiac development and for basal heart homeostasis in adult mice. Cardiac-specific deletion of Hippo kinase mammalian sterile 20-like 1 or 2, Mst kinase coactivator Salvador, and large tumor suppressor homologue 2 in mice resulted in overgrown hearts with elevated cardiomyocyte proliferation.8 Consistently, overexpression of YAP in the mouse embryonic heart increased heart size by promoting cardiomyocyte proliferation, whereas inactivation of YAP in the mouse embryonic heart impeded cardiomyocyte proliferation, causing myocardial hypoplasia and embryonic lethality.9,10 Moreover, ablation of YAP in the postnatal mouse heart resulted in dilated cardiomyopathy and premature death.11 Conversely, forced expression of YAP in the adult mouse heart stimulated cardiac regeneration and improved contractility after myocardial infarction.12 Despite significant progress in defining the importance of Hippo–YAP signaling in the mouse heart, the functional role of YAP in the vascular development remains elusive.

Our recent study showed that YAP plays a novel integrative role in smooth muscle phenotypic modulation by inhibiting smooth muscle–specific gene expression while promoting smooth muscle proliferation and migration in vitro and in vivo.13 Specifically, knockdown of YAP expression in rodent carotid artery injury models attenuated injury-induced smooth muscle dedifferentiation and neointima formation.13

During vasculogenesis, SMCs are highly proliferative and migratory, similar to the synthetic phenotype that SMCs exhibit in response to arterial injury. At the molecular level, nonmuscle myosin heavy chain IIB, which is highly expressed in embryonic SMCs during early vasculogenesis and turned off in mature vascular SMCs, is re-expressed in proliferating SMCs after arterial injury and in atherosclerosis.14,15 Because of the similarities between embryonic SMCs and phenotypically modified adult SMC, we sought to explore the function of YAP in vascular development in vivo using YAP conditional mutant mice in which YAP is specifically ablated in cardiomyocytes and vascular SMCs. We found that loss of YAP in murine cardiac muscle and vascular SMCs resulted in perinatal lethality. YAP conditional deletion mice exhibited severe vascular phenotypes including thinning of the vascular wall, retroesophageal right subclavian artery (RSA), and short/absent brachiocephalic artery. YAP conditional knockout (KO) mice also exhibited complex cardiac phenotypes including hypoplastic myocardial wall, membranous ventricular septal defects, and double outlet right ventricle because of the transient Cre activation in embryonic mouse heart. Moreover, we found that YAP deletion had no effects on vascular smooth muscle differentiation but instead significantly attenuated SMC proliferation, at least in part, through upregulation of the cell cycle arrest gene G-protein–coupled receptor 132 (Gpr132). YAP recruited histone deacetylase (HDAC)-4 to form a transcriptional repressor complex on the Gpr132 gene promoter to repress Gpr132 expression. Collectively, this study not only suggests a crucial role of YAP in smooth muscle development but also provides a novel mechanistic insight into YAP-mediated SMC proliferation.

Methods

Cardiac/smooth muscle–specific YAP KO mice were generated by crossing YAP flox mice16 with SM22α-Cre transgenic mice.17 Both YAP flox and SM22α-Cre mice were maintained in C57BL/6 strain background. Full materials and methods are detailed in the online-only Data Supplement.

Results

Conditional Deletion of YAP in Cardiac/SMCs in Mice Results in Perinatal Lethality

Early lethality observed in global YAP-null embryos precluded assessment of the function of YAP in vascular smooth muscle development.18 To determine the role of YAP in cardiac/vascular SMC development, YAPF/F mice were crossed with transgenic mice expressing Cre recombinase under the control of the SM22α gene promoter. This resulted in deletion of YAP in embryonic heart and vascular SMCs (Online Figure IA).17 Progenies of SM22α-Cre+/YAPF/W mice intercrossed with YAPF/F mice exhibited the expected 25% Mendelian ratio from E10.5 to E15.5. Starting from E16.5 to postnatal day (P) 0, a significant reduction of SM22α-Cre+/YAPF/F mutants (KO) observed. From P1 to P14, only 1 mutant among 107 offspring survived but died on P21 (Online Table I). These data demonstrated that conditional deletion of YAP in embryonic mouse cardiac/vascular SMCs results in perinatal lethality. The P0 neonatal mutants died within hours after birth with marked cyanosis (Online Figure II), suggesting that the perinatal lethality may be attributable to cardiovascular defects as anticipated.

Specific Ablation of YAP in Cardiac/Vascular SMCs Results in Cardiac Defects That Are Associated With Reduced Proliferation

Although SM22α is well documented as a marker of smooth muscle, it is, like most other markers for smooth muscle lineage, transiently expressed in the heart between E8.5 to E12.5 in mice.19 Therefore, in addition of vascular SMCs, we expect there will also be deletion of YAP in embryonic heart. Compared with littermate control embryos, there was a significant decrease of immunoblot and immunohistochemistry signal in cardiac and vascular SMCs of YAP mutants, although some YAP signal remained because of a possible incomplete deletion and mix of cell types in lysates used for Western blotting (Online Figure IB and IC). Consistent with previous studies demonstrating critical role of YAP in cardiac development in mice,9,10 histology analysis revealed that all of YAP mutant embryos and new born pups examined (100%; n=21/21; E14.5 to P0) displayed membranous ventricular septal defect and double outlet right ventricle with hypoplastic myocardial wall (Figure 1). YAP mutants also exhibited significant thinning of right and left ventricular wall from E11.5 to P0 (Online Figure III), at least partially resulting from reduced cardiomyocyte proliferation as shown by decreased Ki67 and phosphohistone 3 positive cardiomyocytes (Online Figure IVA–IVD). Similarly, the ventricular septal defect phenotype could be a result of hypoplasia of septal cardiomyocytes because there was significantly less Ki67 and phosphohistone 3 staining in this region of heart (Online Figure IVE and IVF).

Figure 1.
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Figure 1.

Cardiac pathology in conditional yes-associated protein (YAP) deletion mutants. A, Example of an E14.5 YAP knockout (KO) heart that demonstrates double outlet right ventricle with the aorta rising from the right ventricle (RV), whereas the aorta derives from left ventricle (LV) in control embryos. AV indicates aortic valve (arrow; scale bar, 500 μm). B, Representative hematoxylin and eosin–stained images showing membranous ventricular septal defect (VSD, arrow) in the hearts of E14.5 and postnatal day 0 (P0) YAP KO mice (scale bar, 500 μm).

Specific KO of YAP in Cardiac/Vascular SMCs Leads to Vascular Morphological Defects, Thinning of Vessel Walls, and Aneurysms

Approximately 33% (n=8 of 24) of YAP KO mice displayed retroesophageal RSA, whereby the RSA abnormally arose from the aortic arch in E14.5 and E18.5 mutant embryos (Figure 2A). Of note, the abnormal origin and route of the RSA caused the trachea to be off its normal midline location, thereby squeezing the esophagus in the E18.5 mutant embryos (Figure 2A). Seventeen percentage (n=4 of 24) of YAP mutants displayed short (Figure 2B) or absent (Figure 2C) brachiocephalic artery in the E18.5 or E13.5 mutant embryos, respectively. Hematoxylin and eosin staining of the left carotid artery and thoracic artery revealed a marked thinning of the arterial wall and aneurismal enlargement of vessel lumen in E15.5 YAP mutant embryos (Figure 3). Similar changes were observed in E13.5 to P0 mutant embryos (data not shown).

Figure 2.
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Figure 2.

Conditional deletion of yes-associated protein (YAP) in cardiac and vascular smooth muscle cells leads to vascular abnormalities. A, Representative hematoxylin and eosin (HE) staining sections to show the retroesophageal right subclavian artery (RSA) phenotype in E14.5 and E18.5 YAP mutants in which the RSA abnormally branches from the aortic arch (AA) and travels behind of esophagus (ES) and trachea (T) (scale bar, 500 μm). B, Posterior view of dissected control and YAP mutant heart and outflow tract showing a short brachiocephalic artery (BA) and abnormally shaped heart in the knockout (KO) embryo. C, HE staining of serial sections of E13.5 KO and control embryos. In the mutant embryo, the branchiocephalic artery (BA) is absent, and the RSA directly arises from the AA, whereas in control embryos, the RSA and right common carotid artery (RCA) form BA (scale bar, 500 μm). LSA indicates left subclavian artery.

Figure 3.
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Figure 3.

Yes-associated protein (YAP) knockout (KO) mice have thin arterial walls and dilated arteries. A, Hematoxylin and eosin–stained images of the left carotid artery (LCA) or thoracic artery (TA; D) from E15.5 control and YAP KO embryos. The thickness of medium wall and size of lumen area were quantified and plotted as depicted in B, C, E, and F, respectively. Data were collected from 3 control and 5 KO embryos (*P<0.05; scale bar, 50 μm).

Deletion of YAP in Vascular SMCs Impairs SMC Proliferation in a Cell-Autonomous Manner

Because specific deletion of YAP in vascular SMCs resulted in a thinning of the arterial wall, we next sought to determine whether this was because of an impairment of SMC proliferation. SM α-actin and Ki67 coimmunostaining revealed that YAP KO mutants exhibited significantly lower SMC numbers and decreased smooth muscle proliferation in the left carotid artery (Figure 4A–4C; Online Figure VB). To investigate further the effect of YAP deficiency on smooth muscle proliferation, SMCs were isolated from the dorsal aorta of E15.5 mutant and control littermate embryos. First we confirmed >99% SMC purity in the culture by smooth muscle myosin heavy chain staining (Online Figure VI). Next bromodeoxyuridine incorporation assay was performed to assess SMC proliferation in vitro. Data from this experiment demonstrated that there was significantly less bromodeoxyuridine incorporation into SMCs derived from YAP-specific KO embryos compared with control littermates (Figure 4D and 4E). Furthermore, deletion of YAP in mouse vascular SMCs significantly impaired cell growth during in vitro cell culture (Figure 4F). Taken together, these data demonstrate that YAP is required for vascular SMC proliferation in a cell-autonomous manner.

Figure 4.
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Figure 4.

Deletion of yes-associated protein (YAP) inhibits vascular smooth muscle cell (SMC) growth in a cell-autonomous manner. A, Sections were prepared from left carotid artery of E14.5 control or YAP knockout (KO) embryos and then immunostained with antibodies for the smooth muscle differentiation marker SM α-actin (red) and the cell proliferative marker, Ki67 (green), as indicated. Samples were counterstained with 4',6-diamidino-2-phenylindole (DAPI; blue) to visualize nuclei. The percentage of Ki67-positive SMCs and numbers of SM α-actin–positive cells in the arterial wall were quantified and plotted in B and C, respectively (n=3 embryos per genotype; *P<0.05; scale bar, 50 μm). D, Vascular SMCs were prepared from E15.5 control or YAP KO dorsal aorta and treated with bromodeoxyuridine (BrdU) for 16 hours and immunostained with anti-BrdU antibody for BrdU-positive cells (BrdU, red; DAPI, blue). The fraction of BrDU-positive cells was quantified and shown in E (scale bar, 50 μm). F, Vascular SMCs from E15.5 control or YAP KO dorsal aorta were plated at equal density at day (D) 0 and then counted at D3, D6, and D9 as indicated (*P<0.05).

Deletion of YAP in Vascular SMCs Specifically Enhances Expression of a Subset of Cell Cycle Arrest Genes Without Affecting SMC Differentiation

Previously, we have shown that knockdown of YAP in cultured SMCs and in rat balloon injury model significantly upregulates smooth muscle marker expression.13 However, immunofluorescence staining (Figure 4A; Online Figure VA and VC), quantitative reverse transcription polymerase chain reaction (Figure 5A), and Western blotting (Figure 5B and 5C) did not show any significant change in smooth muscle differentiation markers in YAP mutant aortic arteries. These data suggest that YAP is dispensable for vascular SMC differentiation whereas is critical for SMC proliferation during development in vivo. No obvious differences in SMC apoptosis were observed between mutant and control embryos as determined by terminal deoxynucleotidyl transferase dUTP nick end labeling staining (data not shown). To explore the mechanism by which deletion of YAP impair SMC proliferation, a polymerase chain reaction–based array was performed to analyze expression of cell cycle related genes in aortic tissues from E15.5 YAP KO and control littermates (Online Table II). Quantitative reverse transcription polymerase chain reaction and Western blotting were then used to confirm the array results (Figure 5). A subset of cell cycle arrest genes including growth arrest– and DNA damage–inducible protein-α (Gadd45α), schlafen 1 (Slfn1), Gpr132, transformation related protein 63 (Trp63), and stratifin were induced significantly in YAP KO aortic tissues, whereas there were no changes detected in the cell cycle genes that promote cell proliferation including Ccnd1, Ccna1, and Foxm1 (Figure 5A–5C). We chose Gpr132 for further characterization because this gene was the most significantly upregulated in YAP mutants and its function is unknown in vascular SMCs. To confirm the effects of YAP on Gpr132 expression, retrovirus expressing YAP was transduced into rat primary SMCs, and Western blot was performed to examine endogenous Gpr132 expression. Data from this experiment revealed that overexpression of YAP significantly suppresses Gpr132 expression (Figure 5D). Taken together, data from these in vivo loss-of-function and in vitro gain-of-function assays demonstrate that YAP is a potent repressor for expression of cell cycle arrest gene Gpr132.

Figure 5.
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Figure 5.

Deletion of yes-associated protein (YAP) induces a subset of cell cycle arrest gene expression without affecting expression of smooth muscle (SM)–specific genes. A, Total RNA was harvested from E15.5 control (open bars) YAP knockout (KO; hatched bars) dorsal aorta, and quantitative reverse transcription polymerase chain reaction was performed to assess gene expression as indicated. The level of gene expression in control vessels was set to 1 (solid line; n=5 to 7 embryos per genotype; *P<0.05). B, A representative Western blot using protein lysates from E15.5 control or YAP KO dorsal aorta tissue is shown. C, Immunoblot signals were normalized to α-tubulin loading control then expressed relative to signals from control aortae (set to 1, solid line; n=4 embryos per genotype; *P<0.05). D, Empty retrovirus or retrovirus encoding YAP was transduced into rat primary aortic SM cells (SMCs), and then G-protein–coupled receptor 132 (Gpr132) expression was analyzed by Western blotting. An arrow points to the endogenous YAP band. Ccnd1 indicates cyclin d1; Hic-5, hydrogen peroxide–inducible clone 5; MLCK, myosin light chain kinase; PCNA, proliferating cell nuclear antigen; Sfn stratifin; and Trp63, transformation related protein 63.

Gpr132 Suppresses SMC Proliferation

Data described above indicated that ablation of YAP in vascular SMCs induces cell cycle arrest gene Gpr132 expression. We first sought to determine whether decreasing Gpr132 would mimic the effects of YAP to promote SMC proliferation.13 Knockdown of Gpr132 in rat vascular SMCs resulted in a significant increase of bromodeoxyuridine incorporation and SMC growth (Figure 6A–6C). Because the Gpr132 was induced after deletion of YAP in mice, we next sought to examine whether overexpression of Gpr132 can attenuate SMC proliferation. Cell cycle analysis demonstrated that Gpr132 overexpressing SMCs had a higher proportion of cells in G0/G1 phase but a lower proportion in G2/M phase (Figure 6D), indicating a cell cycle arrest in G0/G1 phase. This cell cycle retardation led to a significant decrease cell growth in the SMCs overexpressing Gpr132 (Figure 6E). Furthermore, silencing Gpr132 rescued the decrease in proliferation observed in YAP KO SMCs while promoted the growth of wild-type SMCs (Figure 6F). Taken together, these data suggest that YAP deletion–induced impairment of SMC proliferation is, at least in part, mediated through induction of the cell cycle arrest gene Gpr132.

Figure 6.
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Figure 6.

G-protein–coupled receptor 132 (Gpr132) suppresses smooth muscle cell (SMC) growth. A, After transfected with control or Gpr132 small interfering RNA duplexes, rat primary aortic SMCs were incubated with bromodeoxyuridine (BrdU) for 24 hours, and then immunostaining was performed to BrdU-positive SMCs by using an anti-BrdU antibody (red) and costained with 4',6-diamidino-2-phenylindole(blue) for nuclei (scale bar, 50 μm). B, The fraction of BrdU-positive SMCs was counted and plotted. C, After silencing Gpr132, rat primary aortic SMCs were seeded at equal density at day (D) 0 and counted at each time point as indicated (n=3; *P<0.05). D, After transduction with Gpr132 or control green fluorescent protein (GFP) adenovirus, rat primary aortic SMCs were harvested for propidium iodide staining to analyze cell cycle by a flow cytometry (*P<0.05). E, After infection with Gpr132 or GFP control adenovirus, rat primary aortic SMCs were seeded at equal density and counted at each time point indicated (*P<0.05). F, E15.5 dorsal aortic SMCs from yes-associated protein knockout (KO) or control embryos were transfected with scrambled control or Gpr132 silencing duplexes. After 24 hours, cells then were plated and counted at each time point as indicated (*P<0.05, control-siControl vs control-siGpr132; #P<0.05, KO-siControl vs KO-siGpr132).

YAP Inhibits Gpr132 Expression via Binding to Gpr132 Gene Promoter

YAP promoted cardiomyocyte proliferation via interaction with TEAD1,9 thus we next sought to explore whether YAP suppresses Gpr132 expression through TEAD1. In support of this possibility, analysis of the mouse Gpr132 revealed an evolutionally conserved TEAD binding muscle CAT element (MCAT) CATN(T/C)(T/C) element in the first intron of the Gpr132 gene (Figure 7A). To experimentally validate that YAP regulates Gpr132 through the MCAT element, we generated wild-type or MCAT mutation Gpr132 luciferase reporter genes, and dual luciferase assays were performed. Overexpression of YAP slightly, although significantly, inhibited Gpr132 reporter gene activity by 20% to 30% but not MCAT mutation reporter (Figure 7B). Because YAP has a moderate suppressing effects on Gpr132 promoter activity but KO of YAP led to more significant Gpr132 expression in vivo (Figure 5), we reasoned that endogenous YAP may cooperate with repressors to downregulate Gpr132 expression. Previous study has shown that selective inhibition of class I and II HDACs inhibits SMC proliferation.20,21 HDAC4 was not only well known for gene transcriptional inactivation but also highly expressed in vascular SMCs and reported to regulate SMC proliferation.21,22 We next determined whether HDAC4 is involved in YAP-mediated Gpr132 gene promoter repression. Luciferase reporter assays demonstrated that, indeed, HDAC4 augmented YAP-mediated repression of Gpr132 promoter activity compared with YAP alone (Figure 7B). In contrast, the MCAT mutant Gpr132 promoter was completely refractory to YAP or HDAC4-mediated repression (Figure 7B). Moreover, data from chromatin immunoprecipitation assays unveiled an ≈3- to 4-fold enrichment of YAP compared with the MCAT region but not exon 3 of Gpr132 gene, suggesting that YAP specifically binds to the MCAT region of the Gpr132 gene (Figure 7C). Furthermore, overexpression of YAP promoted HDAC4 binding to MCAT region of Gpr132 gene (Figure 7D). Because these data suggested that YAP recruits HDAC4 to inhibit Gpr132 gene promoter activity, we sought to determine whether YAP can bind to HDAC4 to form a complex in vivo. YAP was transduced into rat primary aortic SMCs, and endogenous HDAC4 was immunoprecipitated with anti-HDAC4 antibody. Western blotting of the HDAC4 immunoprecipitates revealed the presence of YAP and TEAD1 together with HDAC4 in the vascular SMCs (Figure 7E). Moreover, data from sequential chromatin immunoprecipitation assays revealed that YAP and HDAC4 co-occupied over the MCAT region within the Gpr132 gene in the primary vascular SMCs (Figure 7F). Collectively, these data demonstrate that YAP recruits HDAC4 binding to the MCAT element within the Gpr132 gene to repress Gpr132 expression, thereby rendering cell cycle arrest. Conversely, KO of YAP liberates the HDAC4-mediated inhibitory effects on Gpr132 gene expression, resulting in a decreased SMC growth because of the cell cycle arrest and ultimately leading to a hypoplastic arterial wall phenotype (Figure 7G).

Figure 7.
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Figure 7.

Yes-associated protein (YAP) recruits histone deacetylase-4 (HDAC4) to a muscle CAT element (MCAT) element to suppress G-protein–coupled receptor 132 (Gpr132) promoter activity. A, Schematic diagram of the Gpr132 gene structure showing a conserved MCAT element within intron 1. A, Wild-type or MCAT mutant reporter (mutated nucleotide in MCAT element is underscored) containing a 1.5k bp fragment within Gpr132 gene intron 1 was generated into pGL2E luciferase vector. The location of 2 sets of primers (PS), localized either in intron 1 or exon (E) 3 used for chromatin immunoprecipitation (ChIP) assays in C, is shown. B, Wild-type or MCAT mutant Gpr132 promoter reporter genes were transfected into pulmonary arterial cultured-1 smooth muscle cells (SMCs) together with YAP in the absence or presence of HDAC4 expression plasmid. Values are presented as relative luciferase activity compared with control vector (set to 1) and are the mean±SE of 6 samples. C, Adenovirus expressing green fluorescent protein (GFP) or Flag-tagged YAP was transduced into rat primary rat aortic SMCs, and then chromatin was harvested for immunoprecipitation with anti-Flag antibody. The precipitated DNA was amplified by a real-time polymerase chain reaction (PCR) using Gpr132 gene-specific primers that span the intronic MCAT region (primer set [PS]1) or exon 3 (PS2) as depicted in A. The YAP binding is expressed relative to GFP control sample (set to 1; *P<0.05). D, ChIP assay was performed as described in C, except that anti-HDAC4 antibody was used to immunoprecipitate endogenous HDAC4 and real-time PCR was performed using PS1 primers (*P<0.05). E, Adenovirus expressing YAP was transduced into rat aortic SMCs, and endogenous HDAC4 was immunoprecipitated by using anti-HDAC4 antibody. Western blot was then performed by using the indicated antibodies. A species-matched IgG served as control. F, Chromatin was harvested from SMCs overexpressing YAP. The chromatin was immunoprecipitated first with anti-YAP antibody, eluted, and then immunoprecipitated with either IgG or anti-HDAC4 antibody. Immunoprecipitated DNA was then analyzed by quantitative PCR using PS1 primers (*P<0.05). G, Schematic diagram depicting the findings of this study.

Discussion

This study provides the first evidence demonstrating a critical role for YAP during smooth muscle development through regulating SMC proliferation. We found that loss of YAP in murine cardiovascular system results in perinatal lethality (Online Figure II and Online Table I). YAP conditional KO mice exhibit severe vascular abnormalities including thinning vascular walls, retroesophageal RSA, and short/absent brachiocephalic arteries (Figure 2). Consistent with attenuated SMC proliferation during development after loss of YAP, we have previously shown that YAP promotes SMC proliferation after vascular injury.13 Although in the current study we found that YAP deletion had no effects on vascular smooth muscle differentiation during embryonic development (Figures 4 and 5; Online Figure V), we previously found that YAP suppresses expression of smooth muscle differentiation genes in adult smooth muscle tissues and cells.13 One possible explanation for this difference could be that there is an incomplete deletion of YAP in vascular SMCs mediated by SM22α promoter–driven cre transgene. In support of this notion, data from quantitative reverse transcription polymerase chain reaction (Figure 5A), Western blotting (Figure 5B and 5C), and immunohistochemistry staining (Online Figure IB) showed that there is a significant amount of residual YAP in mutant vascular SMCs. Alternatively, it is possible that programs governing smooth muscle development and those governing smooth muscle dedifferentiation in response to arterial injury in adult mice are distinct. Although there are some common mechanisms shared by both processes, the transient phenotypic switch associated with repair of vascular injury does not exactly recapitulate regulatory programs involved in controlling SMC differentiation during vascular SMC development.23 In support of this, we found that Gpr132 expression was downregulated after arterial injury in concomitant with upregulation of YAP. However, knockdown of YAP in the arterial injury model did not reverse the Gpr132 expression (data not shown), suggesting that there are distinct regulatory mechanisms for Gpr132 expression in either YAP-dependent or -independent manner.

SM22α-Cre transgenic mice generate highly efficient Cre-mediated recombination as early as E9.5 in cardiomyocytes, mesoderm, and neural crest–derived SMCs.17,24,25 Neural crest cells significantly contribute to craniofacial and cardiovascular development by migrating through the pharyngeal arches. Cardiac neural crest, a specific population of neural crest cells, gives rise to the third, fourth, and sixth pharyngeal arches. Among them, the right fourth pharyngeal artery forms the base of the RSA.26 Previous studies have shown that disruption of the migration, proliferation, and differentiation of neural crest–derived SMCs in pharyngeal arteries will cause a variety of congenital outflow tract defects.26 The observed specific defects such as retroesophageal RSA and short/absent brachiocephalic artery in SM22α-Cre+/YAPF/F mutants suggest that YAP is specifically required for the right fourth pharyngeal artery to form a proper pattern of RSA. Additional studies are needed to delete YAP specifically in neural crest to determine the role of YAP in neural crest–mediated pharyngeal artery development. Furthermore, because of the embryonic and perinatal lethality of the YAP mutant mice directed by the SM22α promoter–driven cre transgene, further study on the role of YAP in adult SMCs will require the use of an inducible KO model.

In this study, we identified a novel mechanism whereby YAP promotes smooth muscle proliferation through inhibiting expression of a subset of cell cycle arrest genes. Previous studies have shown that YAP stimulates cardiomyocyte proliferation through induction of several cell cycle–promoting genes including Aurka, Aurkb, Cdc2, and Cdc20.8,9 Furthermore, our recent study showed that YAP can induce cultured SMC proliferation in vitro by induction of Ccnd1.13 In the current study, however, we showed that none of these cell cycle–promoting genes were altered in the vascular SMCs of YAP KO mice (Figure 5A). Instead, we observed increased expression of a subset of cell cycle arrest genes including Gadd45α, schlafen 1, Gpr132, Trp63, and stratifin (Figure 5A and 5B). Among these genes identified, Gadd45α and Trp63 have been shown to be involved in platelet-derived growth factor BB and estrogen-mediated smooth muscle growth, respectively.27,28 Thus far no studies have been shown that Gpr132 plays a role in SMC proliferation. In this study, we found that forced expression of YAP specifically suppressed Gpr132 expression (Figure 5D) but not other cell cycle arrest genes (data not shown). Furthermore, we found that silencing Gpr132 promoted vascular SMC growth (Figure 6A–6C), a result similar to that seen after overexpression of YAP in vascular SMCs.13 Consistent with previous report showing that ectopic expression of Gpr132 leads to a growth inhibition,29 we found that overexpression of Gpr132 attenuated vascular SMC growth by arresting the cell cycle at G0/G1 phase (Figure 6D–6F). These data suggest that YAP-induced vascular hypoplasia, at least in part, is through upregulation of cell cycle arrest gene Gpr132.

Gpr132, originally identified as a G-protein–coupled receptor for which lysophosphatidylcholine is a high-affinity ligand, belongs to a newly defined lysophospholipid receptor subfamily.30 Previous studies demonstrated that Gpr132 differentially couples to multiple G proteins including Gαs, Gαq, and Gα13 and stimulates intracellular Ca2+ transients and extracellular signal–related kinase phosphorylation.31 Additional studies are wanted to determine the role of lysophosphatidylcholine ligand and dissect the downstream signaling of Gpr132 in the SMC proliferation during smooth muscle development.

In addition to the Gpr132 identified from the array, we found that several other cell cycle arrest genes such as Gadd45α and Trp63 were upregulated in the YAP KO arteries. Mechanistically, YAP recruits HDAC4 to an MCAT element to form a transcriptional repressor complex with TEAD1 on the Gpr132 gene promoter, and the MCAT element within Gpr132 gene promoter is indispensible to confer the YAP inhibitory effect (Figure 7). By a bioinformatics search, we found several putative consensus MCAT elements within the Gadd45α and Trp63 gene promoters (data not shown). Thus, the hypoplastic artery phenotype observed in YAP KO mice is likely because of the combined effects from these cell cycle arrest genes through a similar MCAT-dependent mechanism. Previous studies demonstrated that HDAC2 and HDAC5 play important roles in the smooth muscle differentiation marker SM α-actin expression,32,33 but we found that HDAC2 and 5 have no effects on Gpr132 gene promoter activity (data not shown). This would suggest that HDAC2 and HDAC5 are critical for SMC differentiation by regulating SM-specific gene SM α-actin expression, whereas HDAC4 is important for SMC proliferation by regulating Gpr132 expression. In support of this notion, Usui et al21 reported recently that HDAC4 promoted neointimal hyperplasia through stimulating proliferation of vascular SMCs. Taken together, these studies suggested that HDAC family proteins play distinct roles in smooth muscle differentiation or proliferation.

In summary, this study not only suggests a crucial role of YAP in vascular smooth muscle development in mice but also identifies a novel mechanism through which YAP mediates vascular SMC proliferation.

Acknowledgments

We thank Dr Paul Herring for a critical reading of the article and Dr D.J. Pan for sharing the yes-associated protein flox mice.

Sources of Funding

The project described was supported by a grant (R01HL109605) from the National Heart, Lung, and Blood Institute, National Institutes of Health to J. Zhou.

Disclosures

None.

Footnotes

  • In December 2013, the average time from submission to first decision for all original research papers submitted to Circulation Research was 11.66 days.

  • The online-only Data Supplement is available with this article at http://circres.ahajournals.org/lookup/suppl/doi:10.1161/CIRCRESAHA.114.303411/-/DC1.

  • Nonstandard Abbreviations and Acronyms
    E
    embryonic day
    Gadd45α
    growth arrest– and DNA damage–inducible protein-α
    Gpr132
    G-protein–coupled receptor 132
    HDAC4
    histone deacetylase-4
    KO
    knockout
    MCAT
    muscle CAT element
    P
    postnatal day
    RSA
    right subclavian artery
    SMC
    smooth muscle cell
    TEAD1
    TEA domain family member 1
    Trp63
    transformation related protein 63
    YAP
    yes-associated protein

  • Received January 7, 2014.
  • Revision received January 27, 2014.
  • Accepted January 29, 2014.
  • © 2014 American Heart Association, Inc.

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Novelty and Significance

What Is Known?

  • Vascular smooth muscle cells (SMCs) are major components of blood vessels and contribute to the maintenance of vascular homeostasis.

  • Yes-associated protein (YAP), a downstream effector of Hippo signaling, is critical for organ size control and tumorigenesis.

  • YAP promotes SMC proliferation and migration, thereby mediating neointima formation after arterial injury. However, the role of YAP in vascular SMC development is unknown.

What New Information Does This Article Contribute?

  • Deletion of YAP in vascular SMCs during mouse embryogenesis leads to perinatal lethality and severe vascular defects associated with impaired proliferation of SMCs.

  • Deletion of YAP attenuates SMC proliferation through induction of the cell cycle arrest genes by recruiting the epigenetic repressor histone deacetylase-4.

  • YAP is critical for vascular development by promoting SMC proliferation.

YAP is known for controlling organ size and tumor formation by promoting cell growth. Previously, we found that YAP plays a crucial role in the phenotypic switch of vascular SMCs in response to arterial injury. However, the role of YAP in vascular SMC development is unknown. By using a genetic approach to inactivate YAP in vascular SMCs during mouse embryogenesis, we demonstrate that YAP is critical for vascular SMC development. SMC-specific deletion of YAP results in profound vascular abnormalities, including thin arterial wall, because of the impaired SMC proliferation. In contrast to previous studies showing YAP activates cell growth by induction of cell cycle–promoting gene expression, we found that YAP promotes SMC growth by repression of cell cycle arrest gene expression through a novel mechanism by which YAP recruits the epigenetic repressor histone deacetylase-4. These results suggest that YAP plays a critical role in cardiovascular development by regulating cell cycle progression. Therefore, these findings could provide the molecular basis for a potential diagnostic strategy for congenital cardiovascular disorders in humans.

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Circulation Research
March 14, 2014, Volume 114, Issue 6
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    Deletion of Yes-Associated Protein (YAP) Specifically in Cardiac and Vascular Smooth Muscle Cells Reveals a Crucial Role for YAP in Mouse Cardiovascular DevelopmentNovelty and Significance
    Yong Wang, Guoqing Hu, Fang Liu, Xiaobo Wang, Mingfu Wu, John J. Schwarz and Jiliang Zhou
    Circulation Research. 2014;114:957-965, originally published March 13, 2014
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    Deletion of Yes-Associated Protein (YAP) Specifically in Cardiac and Vascular Smooth Muscle Cells Reveals a Crucial Role for YAP in Mouse Cardiovascular DevelopmentNovelty and Significance
    Yong Wang, Guoqing Hu, Fang Liu, Xiaobo Wang, Mingfu Wu, John J. Schwarz and Jiliang Zhou
    Circulation Research. 2014;114:957-965, originally published March 13, 2014
    https://doi.org/10.1161/CIRCRESAHA.114.303411
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