Abstract 002: Blinded Head-to-Head Comparison of Intracoronary Allogeneic Cardiosphere-Derived Cells (icCDCs) Versus Allogeneic Mesenchymal Stem Cells (icMSCs) in Swine with Hibernating Myocardium
Background: Autologous icCDCs and icMSCs each improve myocardial function by stimulating endogenous myocyte proliferation. Since few myocytes arise directly from the injected cells, we evaluated the feasibility and comparative efficacy of allogeneic icCDCs vs. icMSCs.
Methods: Studies were conducted in a blinded fashion. Immunosuppressed swine (cyclosporine 200 mg/day, N=26) with hibernating myocardium from a chronic LAD stenosis were randomized to receive vehicle, ~35 x 106 icMSCs, or ~35 x 106 icCDCs. Cells were infused into the 3 major coronary arteries at ~106 cells/min under continuous flow conditions. LAD function and pathologic indices of myocyte regeneration and proliferation were assessed 1 month later.
Results: Baseline LAD wall thickening (%WT) was reduced in all animals (LAD 38 ± 2% vs. 83 ± 5% in remote, p<0.01) but not different between treatment groups. One month later, LAD %WT remained depressed in vehicle-treated animals but increased to a similar extent following icCDCs and icMSCs (Table). Intracoronary cell therapy also produced large reciprocal increases in nuclear density and reductions in myocyte size with no differences between cell types. Indices of myocyte proliferation (Ki67 and phospho-Histone H3) remained elevated following icCDC treatment but had returned towards control in icMSC-treated animals.
Conclusion: Our data demonstrate the feasibility of intracoronary allogeneic icCDC and icMSC infusion to treat viable dysfunctional myocardium without employing the “stop-flow” technique. Both cell types produce an equivalent increase in myocyte number and LAD function after 4-weeks but proliferative indices remain elevated after icCDCs.
- © 2013 by American Heart Association, Inc.