Abstract 280: Protection of Oral Hydrogen Water as an Antioxidant on Pulmonary Hypertension
Rationale: This study was to explore protection of hydrogen as an antioxidant on monocrotaline (MCT)-induced pulmonary hypertension (PH). Instead of hydrogen-rich saline, we made new modification by using oral hydrogen water (HW), which is more convenient, less costly and longer time to be released.
Methods: Thirty-six SD rats were equally randomized to three groups: SHAM group, MCT group and MCT+HW group. The PH model was established by intraperitoneal injection of MCT (80 mg/kg body weight). The mean pulmonary arterial pressure (mPAP) was measured two weeks after establishment of the model, ensuring that mPAP in the modeled rats was higher than that in SHAM group. Animals in MCT group were administered with ordinary drinking water, and those in MCT+HW group were administered with HW. Two weeks later, the animals were sacrificed, and the heart and lung tissue were removed for measurement of the ventricular weight, left ventricular+septum weight, and right ventricle weight (RV). The right ventricular hypertrophy index (RVHI) was calculated. Changes in the degree of damage to the lung tissue was observed and scored by HE staining. Changes of 3-nitrityrosine (3-nt) and Intercellular Adhesion Molecule-1 (ICAM-1) in the lung tissue were observed by immunohistochemistry.
Results: By the end of the experiment at day 28, mPAP, RV and RVHI in MCT group were 44.6±7.2 mmHg, 0.438±0.051 g and 0.59±0.065 respectively, which were significant higher than those in SHAM group (P < 0.05). mPAP, RV and RVHI in MCT+HW group were 31.4±6.5 mmHg, 0.346±0.041 g and 0.45±0.048 respectively, which were significantly lower than those in MCT group (P < 0.05). In addition, pulmonary inflammatory response, 3-nt and ICAM-1 were increased significantly in MCT group and decreased significantly in MCT+HW group as compared with SHAM group (both P < 0.05).
Conclusions: Oral hydrogen water has a significant protective effect on MCT-induced PH, which is more convenient to use than hydrogen-rich saline. And this effect is associated with the antioxidative ability of hydrogen and its action of reducing pulmonary inflammatory response.
- © 2012 by American Heart Association, Inc.