Abstract 167: MicroRNA Profiles of Non--ST-Elevation Myocardial Infarction
Significance: MicroRNAs (miRNAs) are short, stable, noncoding translational regulators used as early biomarkers for numerous disease processes. Although specific miRNA patterns are associated with STEMI, the profile and timecourse of miRNA release in NSTEMI is currently unknown but of potential clinical significance.Materials and
Methods: With IRB approval, patients identified with chest pain were identified at the UMass Medical Center. Upon ED arrival (T0), a K2EDTA tube of whole blood was obtained with simultaneous troponin cTnI levels. After a 6 hour (T6) observation period, another additional K2EDTA tube was drawn, again contemporaneous with cTnI levels. Patients were selected based on an elevated cTnI level (greater then or equal to ≥ 0.04 ng/ml) at T6 as compared to a negative cTnI levels (< 0.04 ng/ml) at T0. MiRNA was isolated from patient plasma using a Trizol extraction procedure. Real-time reverse transciptase polymerase chain reaction (QRT-PCR) was performed for 94 a priori miRNAs common to cardiovascular development or disease.
Results: Of the 94 miRNAs examined, 11 demonstrated greater than 2-fold change as compared to baseline (Figure 1). A subset of this cohort, namely miRNAs 208a, 483-5p, and 601 revealed statistically significant fold difference (p< 0.05). MiRNA 208a is associated with acute MI. MiRNA 601 has not been previously shown correlated with heart disease.
Conclusion: This investigation reveals a novel 11 member miRNA profile, including miRNA 208a, 483-5p, and 601, which is associated with NSTEMI. Our findings suggest this miRNA profile by be used as a potential biomarker of myocardial injury.
- © 2012 by American Heart Association, Inc.