Stability of Fibrin Contiguous to Intima of Veins
A technique is described which employs an everted segment of canine jugular vein to study the effect of the intima on contiguous fibrin. Following eversion of the vein on a stainless steel rod of suitable dimensions, the vein was coated with a layer of fibrin by winding it onto the endothelial surface. The rate at which soluble Folin-Ciocalteu reactive material appeared in the incubated media of clots so prepared was significantly higher than that developed in systems containing either everted vein alone or fibrin alone. EACA suppressed the liberation of soluble tyrosine from contiguous fibrin but was without effect on the liberation of soluble Folin-Ciocalteu reactive material from the vein alone.
- Received May 23, 1962.
- © 1962 American Heart Association, Inc.