Metabolism of Differently Labeled Cholesterol in Normal and Hypercholesteremic Rabbit
C-4- and C-26-labeled cholesterols were fed via stomach tube to normal and several groups of hypercholesteremic rabbits. The serum, liver, and aortic cholesterol levels were measured, as were the liver and intestinal content and stool nonsaponifiable and saponifiable lipid fractions. The appearance of the isotopic cholesterol in these fractions and in the exhaled CO2 was measured. The recovery rates of the cholesterol -26-C14 was 20.0 to 38.3 per cent, and of the cholesterol -4-C14, 79.0 to 109.2 per cent. The cholesterol-fed animals showed distinctly higher radioactivity in the blood and in the aorta. The C-26, but not the C-4, activity was detected in the exhaled CO2. No activity was detected in the urine. Both tags were detected in the saponifiable fractions of the liver and intestinal contents. Arachidonic acid supplements to four rabbits resulted in increased excretion of activity in the saponifiable portion of the intestinal contents.
- Received March 8, 1962.
- © 1962 American Heart Association, Inc.