Abstract P026: Development of Autologous Tissue Small-Diameter Vascular Grafts (BIOTUBEs)
Objectives: There are actually no small-caliber synthetic vascular grafts (< 6 mm) with acceptable patency rate for the use of coronary bypass or peripheral vascular repair below the knee in case the autologous vessels are not available. We developed autologous small-caliber vascular grafts “BIOTUBEs” using simple, safe and economical in vivo tissue engineering. In this study, we summarize the development of BIOTUBEs.
Methods: Silicone rod molds (diameter: 1.5∼5 mm, length: 20∼50 mm) were placed into subcutaneous pouches of Wister rats, Japan white rabbits or Beagle dogs. After 1 month, BIOTUBEs formed around the molds were auto-implanted to the aorta (1.5 mm; rats) or the carotid arteries (2 mm; rabbits and 5 mm; dogs) of the respective animals. They were evaluated after determined period of implantation.
Results: Irrespective of species, BIOTUBEs had thin wall (ca. 0.1mm) and mainly consisted of autologous fibroblasts and collagen fibers. Rats; After 12-week implantation, other than the oriented endothelial layer and smooth muscle layer, multilayered elastin fiber formation was observed in the grafts. Rabbits; Little thrombus was formed on the luminal surfaces completely covered with endothelial cells within 2 weeks. During 2 year-implantation, neither formation of aneurysms nor rupturing was observed in BIOTUBEs. Dogs; Longest follow up is 3 years under arterial pulsatile pressure condition without any degenerative changes in the grafts.
Conclusions: BIOTUBEs could be ideal small caliber vascular prostheses that greatly facilitate healing process and exhibit excellent biocompatibility.
- © 2011 by American Heart Association, Inc.