Abstract P005: Direct Differentiation of Atrial and Ventricular Myocytes from Human Embryonic Stem Cells
Although cell transplantation studies have suggested promising therapeutic potentials for myocardial infarction, the leading cause of death worldwide, the incapability to obtain relatively homogeneous ventricular myocytes for transplantation is one major obstacle to the development of clinical therapies for myocardial repair1. Human embryonic stem cell (hESC) is a promising source of cardiomyocytes. Here we show that both Noggin and pan-retinoic acid receptor antagonist BMS-1894532 (RAi) significantly increase the cardiac differentiation efficiency of hESCs. Investigating retinoid function by comparing Noggin+RAi-treated cultures with Noggin+RA-treated cultures, we found that with 64±0.88% (mean ±s.e.m) cardiac differentiation efficiency, 83% of the cardiomyocytes in Noggin+RAi-treated cultures had embryonic ventricular-like action potentials (AP); while, with 50±1.76% cardiac differentiation efficiency, 94% of those in Noggin+RA-treated cultures had embryonic atrial-like APs. These findings demonstrating that relatively homogeneous embryonic atrial and ventricular myocyte populations can be efficiently derived from hESCs by specifically influencing signaling cascades.
- © 2011 by American Heart Association, Inc.