S100A4 and Bone Morphogenetic Protein-2 Codependently Induce Vascular Smooth Muscle Cell Migration via Phospho–Extracellular Signal-Regulated Kinase and Chloride Intracellular Channel 4
Rationale: S100A4/Mts1 is implicated in motility of human pulmonary artery smooth muscle cells (hPASMCs), through an interaction with the RAGE (receptor for advanced glycation end products).
Objective: We hypothesized that S100A4/Mts1-mediated hPASMC motility might be enhanced by loss of function of bone morphogenetic protein (BMP) receptor (BMPR)II, observed in pulmonary arterial hypertension.
Methods and Results: Both S100A4/Mts1 (500 ng/mL) and BMP-2 (10 ng/mL) induce migration of hPASMCs in a novel codependent manner, in that the response to either ligand is lost with anti-RAGE or BMPRII short interference (si)RNA. Phosphorylation of extracellular signal-regulated kinase is induced by both ligands and is required for motility by inducing matrix metalloproteinase 2 activity, but phospho–extracellular signal-regulated kinase 1/2 is blocked by anti-RAGE and not by BMPRII short interference RNA. In contrast, BMPRII short interference RNA, but not anti-RAGE, reduces expression of intracellular chloride channel (CLIC)4, a scaffolding molecule necessary for motility in response to S100A4/Mts1 or BMP-2. Reduced CLIC4 expression does not interfere with S100A4/Mts1 internalization or its interaction with myosin heavy chain IIA, but does alter alignment of myosin heavy chain IIA and actin filaments creating the appearance of vacuoles. This abnormality is associated with reduced peripheral distribution and/or delayed activation of RhoA and Rac1, small GTPases required for retraction and extension of lamellipodia in motile cells.
Conclusions: Our studies demonstrate how a single ligand (BMP-2 or S100A4/Mts1) can recruit multiple cell surface receptors to relay signals that coordinate events culminating in a functional response, ie, cell motility. We speculate that this carefully controlled process limits signals from multiple ligands, but could be subverted in disease.
- bone morphogenetic protein
- S100 protein
- vascular smooth muscle cells
- intracellular chloride channel
S100A4 (also known as Mts1, metastasin, p9Ka, pEL98, CAPL, calvasculin, Fsp-1, placental calcium-binding protein) belongs to the family of EF-hand calcium-binding proteins.1 Increased expression of S100A4/Mts1 is observed in cancer cells and contributes to tumor cell motility and metastatic progression, through interaction with myosin heavy chain II.2 Our group has shown that S100A4/Mts1 also induces human pulmonary artery smooth muscle cell (hPASMC) migration and proliferation by interacting with RAGE (receptor for advanced glycation end products).3 Migration and proliferation of hPASMCs have been linked to the pathogenesis of pulmonary arterial hypertension (PAH).4 This is in keeping with our previously reported finding that a small percentage of transgenic mice overexpressing S100A4/Mts1 develop occlusive pulmonary vascular changes.5 We have also previously reported that expression of S100A4/Mts1 requires a cooperative interaction between the serotonin receptor and transporter.3 This, too, is relevant to the pathogenesis of pulmonary hypertension, because heightened serotonin activity has been linked, both clinically and experimentally, to the pathogenesis of PAH.6
Mutations causing loss of function of bone morphogenetic protein (BMP) receptor (BMPR)II have been linked to >60% of cases of familial PAH and 25% of cases of sporadic PAH, but the penetrance is low,7,8 suggesting that other modifier genes and/or environmental factors may be important. Thus, understanding the interactions between signaling via BMPRII and other cell surface receptors, such as RAGE, via S100A4/Mts1 is helpful in uncovering factors that modify propensity to vascular disease in association with a BMPRII mutation.
Our studies reveal a previously unknown codependence between signals emanating from BMPRII and RAGE in mediating cell motility. Suppression of RAGE, but not BMPRII, resulted in loss of phospho–extracellular signal-regulated kinase (pERK) and matrix metalloproteinase (MMP)2 activity, whereas loss of BMPRII but not RAGE reduced levels of the intracellular chloride channel (CLIC)4, a scaffolding protein previously implicated in motility.9,10 Loss of CLIC4 resulted in impaired activation and distribution of the small GTPases RhoA and Rac1, necessary for cell retraction and extension of filopodia and lamellipodia during cell migration.
An expanded Methods section is available in the Online Data Supplement at http://circres.ahajournals.org.
hPASMCs were used between passages 5 and 9, and synchronized using a defined starvation medium. In some experiments, cells were preincubated with the phospho (p)ERK1/2-inhibitor PD 98059 at 25 and 100 μmol/L or with the mitogen-activated protein kinase kinase (MEK) inhibitor U0126 10 μmol/L or with a rabbit polyclonal anti-RAGE antibody.
Migration Assay Using a Modified Boyden Chamber
Cells were added to fibronectin-coated microporous inserts of 24 well plates, and the migratory stimulus was added to the well in the bottom of the chamber for 6 hour. The cells that had migrated to the bottom of the insert were fixed and stained with the Diff Quick Kit. The cells in three different fields (×200 to ×400) at the center of each well were counted under the microscope.
Transfection With Short Interference RNA
To suppress expression of BMPRII and CLIC4 in hPASMCs, we transfected short interference (si)RNA SMARTpool from Dharmacon (Lafayette, Colo) and Lipofectamine 2000 (Invitrogen, Carlsbad, Calif) as described in the Online Data Supplement. Suppression of BMPRII and CLIC4 was documented 48 hour later both by quantitative real-time polymerase chain reaction (PCR) and by western immunoblot.
Western Immunoblotting and Phosphorylation Assays
Cell lysates were prepared and run under reducing conditions before transfer to a nitrocellulose membrane. The membrane was blocked and the blots were incubated with polyclonal antibodies against human CLIC4, S100A4/Mts1 or His tag. Densitometry was carried out using the quantity one software by Bio-Rad (Hercules, Calif).
cDNA Microarray Analysis
Microarray analysis of cDNAs obtained from hPASMCs, data acquisition and analysis were performed as previously described11 comparing genes that were similarly up or down regulated >2 fold by S100A4/Mts1 and BMP-2 and blocked by BMPRII siRNA but not by anti-RAGE.
Quantitative TaqMan Real-Time PCR
RNA was isolated by TRIzol (Invitrogen) and reverse-transcribed using Superscript III (Invitrogen). RT-PCR was performed on a 7900HT Sequence Detection System with TaqMan preverified Assays-on-Demand gene expression probes (Applied Biosystems, Foster City, Calif) for BMPRII (assay ID Hs 01556128_m1), CLIC4 (assay ID Hs 00983246_g1), using the absolute quantification method with β2-microglobulin (assay ID Mm 01269327_g1) as the endogenous control.
hPASMCs were seeded on collagen I–coated 4-chamber slides fixed in 4% paraformaldehyde. We used, a polyclonal rabbit and monoclonal goat CLIC4 antibody, a polyclonal rabbit myosin heavy chain (MHC)IIA antibody, a mouse monoclonal Rac1, and a rabbit polyclonal α smooth muscle actin antibody. Nuclei were counterstained with DAPI. Images were acquired with a Leica microscope using Openlab version 3.1.4 software (Improvision, Coventry, UK).
Luminescence Caspase 3/7 Apoptosis Assay
In PASMCs 48 hours after transfection with control RNA interference (RNAi), BMPRII RNAi and CLIC4 RNAi, caspase 3/7 activity was assessed with Luminescence Caspase-Glo 3/7 Assay (G8091; Promega, Madison, Wis) in a 96-well plate as described in the kit.
Rac1 and RhoA and Cd42 Pulldown Assays
Rac1, RhoA, and Cd42 activation were assayed after stimulation with recombinant S100A4/Mts1, using kits for Rac1 and RhoA (Upstate technology, Temecula, NY) and for Cdc42 (EZ-Detect Cdc42 Activation kit, Pierce, Rockford, Ill).
To assess MMP activity, electrophoresis was carried out on an 8% SDS-PAGE copolymerized with gelatin and stained with 0.05% Coomassie brilliant blue and destained in 5% ethanol with 7.5% acetic acid. Areas of proteolysis were quantified using the public JAVA image-processing program NIH Image/ImageJ.
The number of experiments carried out for each determination is given in the Figure legends. All quantitative results are presented as means±SEM. Statistical significance was determined by 1-way ANOVA, followed by a Bonferroni post hoc test or Dunnett’s when comparisons involved ≥3 groups. A probability value of <0.05 was considered significant.
S100A4/Mts1- or BMP-2–Induced hPASMC Migration Requires RAGE and BMPRII
Stimulation of hPASMCs with recombinant S100A4/Mts1 (500 ng/mL) or with BMP-2 (10 ng/mL) resulted in a 2-fold increase in migration compared to unstimulated (control) cells, as assessed in Boyden chambers at 6 hours. Although we expected that the S100A4/Mts1 migratory response of hPASMCs would be blocked by anti-RAGE, the same was true for BMP-2 mediated hPAMSC migration (Figure 1A). Similarly, when we reduced the levels of BMPRII mRNA and protein12 with siRNA to 20% of those in hPASMCs transfected with control siRNA (Figure 1B), the migratory response to both BMP-2 and to S100A4/Mts1 was repressed (Figure 1C). We previously published that in cells from the same harvests, the reduction in BMPRII protein by siRNA was associated with reduced phosphorylation of Smad 1/5.12 The migratory response to the motogen platelet-derived growth factor (PDGF)-BB was not influenced by knocking down BMPRII (Figure 1D) or by anti-RAGE.3
pERK1/2 Is Necessary but Not Sufficient for S100A4/Mts1- and BMP-2–Induced hPASMC Migration
In a search for common or complementary signaling mechanisms activated via RAGE or BMPRII in response to either S100A4/Mts1 or BMP-2, we identified pERK1/2 (Figure 2A). Other signaling molecules involved in migration (c-Jun N-terminal kinase, p38, Smads) were not induced by both ligands (data not shown). Blockade of pERK1/2 with the specific mitogen-activated protein kinase inhibitor PD98059 at 25 and 100 μmol/L or with the MEK inhibitor U0126 10 μmol/L abrogated the migratory response to both BMP-2 and S100A4/Mts1 (Figure 2B). Interestingly, pERK1/2 was blocked by anti-RAGE but not by BMPRII siRNA, suggesting that both BMP-2 and S100A4/Mts1 activate pERK through RAGE (Figure 2C). In addition, cells that were treated with BMPRII siRNA and stimulated with BMP-2 showed ERK phosphorylation that could be blocked with anti-RAGE, indicating that BMP-2 can induce pERK in a BMPRII independent manner via RAGE (Figure 2D). However, it also implies that, with loss of BMPRII, neither BMP-2– nor S100A4/Mts1-mediated pERK is sufficient to induce the migratory response of hPASMCs. We therefore set out to determine downstream targets of BMPRII important in migration that were influenced by stimulation with both S100A4/Mts1 and BMP-2.
CLIC4 Expression Depends on BMPRII and Is Necessary for S100A4/Mts1- and BMP-2–Mediated Migration of hPASMCs
To identify BMPRII-dependent gene transcripts, we used Stanford cDNA microarrays11 to mine for mRNAs in hPASMCs that were altered by BMPRII siRNA but not by anti-RAGE in response to BMP-2 and S100A4/Mts1 stimulation for 6 hours.
Of all 14 500 annotated genes on the array, 23 were BMPRII-dependent genes meaning that they were similarly expressed after S100A4/Mts1 and BMP-2 stimulation and had a similar alteration in expression pattern with BMPRII siRNA but not the RAGE antibody. Of those, 6 genes have been previously associated with motility (tenascin C, fibrillin I, myosin light chain polypeptide kinase, α-tubulin, Ras-GTPase activating protein, and CLIC4). Of those 6 genes, CLIC4 showed the greatest alteration in expression with loss of BMPRII (4-fold reduction). Confirmation of this reduction in gene expression was verified by quantitative RT-PCR (Figure 3A). Knockdown of CLIC4 following pretreatment with BMPRII siRNA was dependent on a ligand-receptor interaction, because knocking down of BMPRII in the absence of ligand stimulation did not reduce CLIC4 levels (Figure 3A). Migration assays were carried out to test the functional importance of these findings, comparing the response of hPASMCs following knockdown of BMPRII or CLIC4 after transfection with siRNA. We documented a reduction in CLIC4 mRNA to 20% of the values obtained with control siRNA (Figure 3B), as well as a comparable decrease in CLIC4 protein (Figure 3C). We show in Figure 3D that migration of hPASMCs in response to S100A4/Mts1 or BMP-2 was abrogated by CLIC4siRNA. As with BMPRII siRNA, the migratory response to PDGF-BB was not influenced by CLIC4 siRNA (Online Figure I).
Previous reports described the localization of CLIC4 in multiple subcellular compartments.13–15 We observed by immunocytochemistry that there is some nuclear staining of CLIC4 under conditions of serum starvation, but as early as 10 minutes after stimulation of hPASMCs with S100A4/Mts1 or BMP-2, CLIC4 moves to a perinuclear and cytoplasmic location and concentrates at or near cell membranes as well as in filopodia (Figure 3E). We therefore set out to determine why maintaining normal CLIC4 levels and pERK1/2 are necessary for hPASMC migration.
CLIC4 and pERK Do Not Influence S100A4/Mts1 Uptake or Binding to MHCIIA
We investigated whether CLIC4 and/or pERK might be required for internalization of S100A4/Mts1 or for binding of S100A4/Mts1 to MHCIIA, processes implicated in motility. His-tagged S100A4/Mts1 uptake into the cell did not depend on CLIC4 (Online Figure II, A). In addition, blockade of pERK1/2 had no effect on rS100A4/Mts1 uptake (Online Figure II, B). Binding of S100A4/Mts1 to MCHIIA2,16 was also neither dependent on pERK 1/2 (Online Figure II, C) nor on CLIC4 (data not shown).
MMP2 Activity Depends on pERK1/2, Not on CLIC4
Previous studies have shown that pERK is necessary for MMP2-mediated motility in aortic smooth muscle cells.17 We observed in hPASMCs that S100A4/Mts1 mediates pro-MMP2 activity, in a pERK-dependent manner in that the response is lost in cells pretreated with the inhibitor PD98059 (Figure 4). We observed primarily an increase in pro-MMP2, because both the amount and any alteration in the active form of MMP2 was difficult to appreciate by zymography. This observation is consistent with that of other investigators using cytokines to stimulate MMP2 activity in smooth muscle cells (SMCs).18 In contrast, reducing CLIC4 by siRNA did not alter pro-MMP2 activity in response to S100A4/Mts1 (Figure 4).
Loss of CLIC4 Causes Accumulation of MHCIIA Around Vacuolar Structures
Following transfection of cells with either BMPRII siRNA or with CLIC4 siRNA but not control siRNA, changes in the cytoskeleton were observed related to the alignment of MHCIIA (Figure 5A through 5D). Loss of CLIC4 resulted in disruption of the linear pattern of myosin staining and caused accumulation of MHCIIA around vacuolar structures. The vacuolar structures are also apparent by staining with an α smooth muscle actin antibody (Online Figure III). Quantification shows that 50% to 60% of cells with knockdown in BMPRII or CLIC4 show vacuole formation, in contrast to 2% to 3% of cells after transfection with control siRNA (Online Figure V). Cell survival assays measuring caspase 3/7 activity do not show differences between transfected and nontransfected cells, suggesting that the vacuole appearing structures do not reflect cell damage (Figure 5E). We confirmed CLIC4 localization not only in the cell body but also extending to cell processes such as lamellipodia and filopodia (arrows). In addition, we demonstrated by immunocytochemistry that CLIC4 staining is markedly reduced in BMPRII RNAi and CLIC4 RNAi treated cells and that the homogeneous distribution of CLIC4 is interrupted by the vacuolar structures (Figure 5F).
CLIC4 Delays and Alters S100A4/Mts1 Induced RhoA and Rac1 Activation
Stimulation of hPASMC migration with both BMP-2 (not shown) and S100A4/Mts1 resulted in rapid activation of RhoA and Rac1 (Figure 6A and 6B). In contrast, suppression of CLIC4 with siRNA resulted in delayed Rac1 (Figure 6A) and RhoA activities (Figure 6B). We stained cells for Rac1 to assess changes in localization and, interestingly, have found that the distribution of Rac1 is altered after BMPRII and CLIC4 knockdown. In nontransfected or control RNAi cells, Rac1 is located both at the periphery and in the perinuclear region in response to S100A4, whereas with BMPRII or CLIC4 RNAi, the perinuclear localization persists but peripheral staining for Rac1 is markedly reduced (see arrows) (Figure 6C). These data support altered small GTPase signaling in response to downregulation of CLIC4.
In assessing the role of Cdc42, we found that total Cdc42 is increased after CLIC4 siRNA versus control RNAi independent of S100A4/Mts1 stimulation. Active to total Cdc42 in response to S100A4/Mts1 stimulation is not significantly affected by CLIC4 versus control siRNA (Online Figure IV).
We propose a novel codependence between RAGE and BMPRII and indicate how a single ligand (BMP-2 or S100A4/Mts1) can recruit additional cell surface receptors to relay signals necessary to orchestrate a functional response, in this case to coordinate cytoskeletal changes with MMP activity and Rho/Rac activation required for cell motility (Figure 7). We speculate that this carefully controlled process limits signals from multiple ligands but could be subverted in disease.
S100A4/Mts1 was described as a protein differentially regulated in metastatic breast cancer and subsequent experimental animal and clinical studies established this protein as a “metastasis” factor in a variety of cancers.19 Our previous studies indicated that RAGE is the receptor for S100A4/Mts1,3 and work by others showed that S100A4/Mts1 internalization and binding to MHCIIA mediates cell motility.2 That BMP-2 is also a motogen is consistent with studies previously reported in human aortic smooth muscle cells.20 However, in rat aortic smooth muscle cells BMP-2 and BMP-4 were shown to suppress motility but in those studies, the BMPs were only used in conjunction with serum or PDGF-mediated motility and in very high doses of 100 ng/mL.21 BMP-2 promotes mesenchymal cell migration in association with atrioventricular valvulogenesis22 and also induces migration of neural crest cells.23
That S100A4/Mts1-RAGE signals depended on crosstalk with BMPRII is a novel finding, but crosstalk between RAGE and Smad2/3 signals both in cultured vascular smooth muscle cells, as well as in mesangial cells have been related to diabetic vascular disease and nephropathy.24 In work from our laboratory,24a we have shown that BMP-2 mediates motility of endothelial cells in a pSmad 1/5 dependent manner.
It has been suggested that the S100 proteins may have interactions with proteins other than RAGE through the hinge domain, so it is possible that S100A4/Mts1 could interact with BMPRII or with some other receptor that recruits BMPRII, such as BMPRIA or BMPRIB. This is because blocking RAGE does not abrogate S100A4/Mts1-mediated processes that depend on BMPRII such as maintaining CLIC4 levels. Because S100A proteins can interact with lipid rafts25 and because BMPRII exists in lipid rafts,26 it is possible that there is a direct association at the level of the cell membrane. Conversely, it is possible that BMP-2 interacts with RAGE or recruits RAGE in a non–BMPRII-dependent manner, ie, through BMPRIA or BMPRIB and an activin type II receptor27 because the absence of BMPRII does not repress BMP-2–mediated pERK1/2 in contrast to pretreatment with anti-RAGE. Because pERK is known to bind RAGE,28 it is perhaps not surprising that S100A4/Mts1- and BMP-2–mediated motility is pERK-dependent. To further substantiate the role of RAGE in mediating the pERK signal, we have made multiple attempts to reduce RAGE expression using a variety of RAGE siRNAs and transfection strategies but could not successfully knockdown RAGE in PASMCs.
In addition to regulating MHCIIA-mediated motility, S100A4/Mts1 also induces MMP2 activity, which is essential for vascular SMC migration.17 RAGE and MMP2 activity have been implicated in restenosis29 and in atherosclerosis,30 S100A4/Mts1 is thought to be a marker of intimal SMCs31 and mediates cancer cell invasiveness through MMP2.32
Our observation that BMPRII is necessary to maintain levels of CLIC4 is novel and unexpected as was the further observation that reduced levels of CLIC4 are sufficient to suppress motility. These findings are consistent with the association of CLIC4 with a signaling complex required for motility of spermatozoa.9 On the other hand, upregulation of CLIC4 during conversion of fibroblasts to myofibroblasts correlates with reduced motility.10 Thus, the exact role of CLIC4 in cell motility may depend on a particular signaling pathway, cell type, or biological context. CLIC4 is found in lipid rafts33 and acts as a scaffolding protein for protein kinases and phosphatases14 and may play a key role in microtubule dynamics, particularly because it localizes with the centrosome of the cell during division and at the apical junctions of polarized epithelial cells. Most recently, CLIC4 has been shown to associate with G-protein coupled receptors such as the histamine H3 receptor,34 suggesting that CLIC4 could play a role in expression/presentation of receptors at the cell surface. We have shown hat peroxisome proliferator-activated receptor (PPAR)γ is downstream of BMP-212 signaling in SMCs, and loss of PPARβ/δ also decreases CLIC4.35
We observed malalignment of myosin with loss of BMPRII or CLIC4 giving the appearance of “fenestrations” or vacuoles in the cytoskeleton. Although nuclear CLIC4 is associated with apoptosis,15 it is possible that loss of cytoplasmic CLIC4 leads to autophagy and that these vacuolar structures represent autophagosomes induced as a cell survival mechanism. Alternatively, the vacuoles could represent some stage of intracellular lumen formation, because reduction of CLIC4 and a Caenorhabditis elegans CLIC, EXC-4, have been linked to defects in lumen/tube formation in endothelial cells and intracellular secretory canals, respectively.36 Based on these findings, our data indicate that the cytoskeletal changes and intracellular vacuoles are indeed induced by loss of CLIC4. We suggest that this leads to delayed activity of Rac1 and RhoA and alterations in the localization of Rac1, and this impairs migration, because these GTPases are critical regulators of cytoskeletal dynamics and play key roles in cell retraction and extension of filopodia.37 Alternatively, reduced activity of RhoA and Rac1 might reflect loss of the function of CLIC4 act as a scaffolding molecule,14 suggesting that CLIC4 may permit timely activation and translocation of these GTPases. CLICs can interact directly or indirectly with members of the ERM (ezrin–radixin–moesin) family of membrane–cytoskeletal linkers,13 proteins reported to be involved in regulation of signaling by Rho family GTPases. Interestingly, we could not show a significant change in Cdc42 activation yet total Cdc42 seems to be increased with CLIC4 RNAi.
Recent studies in cultured hPASMCs, by our group indicate that loss of BMPRII function increases proliferation in response to agonists such as PDGF-BB.12 Because loss of BMPRII function reduces motility, it is possible that migration is less important than proliferation in the pathogenesis of occlusive lesions in PAH. Alternatively, additional modifications are necessary for loss of function of BMPRII to result in both heightened proliferation and migration, for example, upregulation of RAGE or heightened activity of other mito/motogens such as PDGF-BB that are unimpeded by reduced BMPRII. This would be in keeping with the low penetrance38 of disease in patients with loss of function of BMPRII and with the relatively mild phenotype in mice with haploinsufficiency of BMPRII.6
The authors are indebted to Dr Michal Roof for help with the Figures and with editorial review of our manuscript.
Sources of Funding
Supported by an American Heart Association/Pulmonary Hypertension Association Postdoctoral Fellowship (to E.S.); postdoctoral fellowship from the Institut National de la Sante et de la Recherché Medicale (INSERM) (to C.G.); postdoctoral fellowship from the American Lung Association (to V.P.); postdoctoral fellowships from the Sigrid Juselius Foundation, Instrumentarium Foundation, the Finnish Foundation for Cardiovascular Research, and the Academy of Finland (to T.-P.A.); National Heart, Lung, and Blood Institute National Research Service award T32-HL007708-14 (to J.M.P.); British Heart Foundation Project grant PG/06/125/1633 (to A.L.); The Ohio University Office of Research and Sponsored Programs (to M.B.); and NIH grant 1-R01-HL074186-01 and the Dwight and Vera Dunlevie Endowed Professorship (to M.R.).
Original received May 22, 2008; first resubmission received December 15, 2008; second resubmission received July 17, 2009; revised second resubmission received August 17, 2009; accepted August 19, 2009.
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