Figure 3. FOXO3a blocked angiotensin II–induced CYR61 expression. A, Serial immunoblot analysis of CYR61, phosphorylated (thus inactivated) native FOXO3a and total native FOXO3a after angiotensin II (10−7 mol/L) stimulation. B, Serial immunoblot analysis of CYR61 protein at 2, 4, and 6 hours after angiotensin II stimulation. C, Corresponding immunohistochemistry of FOXO3a and CYR61 2 hours after angiotensin II stimulation in rat aorta that were pretransfected with Ad-GFP (left), Ad-TM-FOXO3a (middle), and Ad-antisense-FOXO3a (right), respectively. Effective transfection of Ad-TM-FOXO3a and Ad-GFP to rat aorta was confirmed by staining for HA, whereas Ad-antisense-FOXO3a does not contain sequences for HA.