A more recent version of this article appeared on April 13, 2001

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(Circulation Research. 2001;0:hh0701.088840.)
© 2001 American Heart Association, Inc.

Online First Article

Gene Transfer of Heterologous G Protein–Coupled Receptors to Cardiomyocytes

Differential Effects on Contractility

Karl-Ludwig Laugwitz¹, Hans-Jörg Weig¹, Alessandra Moretti, Eva Hoffmann, Peter Ueblacker, Ingo Pragst, Kai Rosport, Albert Schömig Martin Ungerer

From the Medizinische Klinik and Deutsches Herzzentrum München (K.-L.L., H.-J.W., A.M., E.H., P.U., A.S.) and Institut für Experimentelle Onkologie und Therapieforschung (I.P.), Technische Universität, Munich, and ProCorde (K.R., M.U.), Martinsried, Germany.

Correspondence to Dr Martin Ungerer, ProCorde, Fraunhoferstrasse 9, D-82152 Martinsried, Germany. E-mail: ungerer{at}procorde.com

Abstract

Abstract—In heart failure, reduced cardiac contractility is accompanied by blunted cAMP responses to ß-adrenergic stimulation. Parathyroid hormone (PTH)–related peptide and arginine vasopressin are released from the myocardium in response to increased wall stress but do not stimulate contractility or adenylyl cyclase at physiological concentrations. To bypass the defective ß-adrenergic signaling cascade, recombinant P1 PTH/PTH-related peptide receptors (rPTH1-Rs) and V₂ vasopressin receptors (rV₂-Rs), which are normally not expressed in the myocardium and which are both strongly coupled to adenylyl cyclase, and recombinant ß₂-adrenergic receptors (rß₂-ARs) were overexpressed in cardiomyocytes by viral gene transfer. The capacity of endogenous hormones to increase contractility via the heterologous, recombinant receptors was compared. Whereas V₂-Rs are uniquely coupled to Gs, PTH1-Rs and ß₂-ARs are also coupled to other G proteins. Gene transfer of rPTH1-Rs or rß₂-ARs to adult cardiomyocytes resulted in maximally increased basal contractility, which could not be further stimulated by adding receptor agonists. Agonists at rPTH1-Rs induced increased cAMP formation and phospholipase C activity. In contrast, healthy or failing rV₂-R–expressing cardiomyocytes showed unaltered basal contractility. Their contractility and cAMP formation increased only at agonist exposure, which did not activate phospholipase C. In summary, we found that gene transfer of PTH1-Rs to cardiomyocytes results in constitutive activity of the transgene, as does that of ß₂-ARs. In the absence of receptor agonists, rPTH1-Rs and rß₂-ARs increase basal contractility, coupling to 2 G proteins simultaneously. In contrast, rV₂-Rs are uniquely coupled to Gs and are not constitutively active, retaining their property to be activated exclusively on agonist stimulation. Therefore, gene transfer of V₂-Rs might be more suited to test the effects of cAMP-stimulating receptors in heart failure than that of PTH1-Rs or ß₂-ARs.

Key Words: gene transfer • receptor • heart failure

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