Published online before print December 13, 2001, doi: 10.1161/hh0202.103647
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Submitted on July 20, 2001
Revised on December 3, 2001
Accepted on December 3, 2001
C/EBP-ß Mediates iNOS Induction by Hypoxia in Rat Pulmonary Microvascular Smooth Muscle Cells
From the Department of Anesthesiology and Critical Care Medicine (X.T., D.L., R.A.J.), Johns Hopkins University School of Medicine, Baltimore, Md; Vascular Biology Center and Department of Pharmacology and Toxicology (J.D.C.), Medical College of Georgia, Augusta, Ga.
* To whom correspondence should be addressed. E-mail: Rajohns{at}jhmi.edu.
Exposure of rats to 10% O2 for 4 days caused pulmonary hypertension and induced expression of both inducible nitric oxide synthase (iNOS) and CCAAT box enhancer binding protein-ß (C/EBP-ß) in rat lung. Electrophoretic mobility shift assays (EMSAs) showed that exposure to 1% O2 increased the C/EBP-ß binding in rat pulmonary microvascular smooth muscle cells (rPSMs). To test the hypothesis that C/EBP-ß participates in hypoxia-induced iNOS expression in rPSMs, a C/EBP motif at -910 bp of rat iNOS promoter was mutated. rPSMs transfected with the rat iNOS promoter and exposed to 1% O2 for 24 hours had significantly increased wild-type iNOS promoter activity. The hypoxia-induced promoter activity was abolished by the C/EBP motif mutation. Thus, C/EBP-ß mediates, at least in part, hypoxia-induced iNOS expression in rPSMs.
Key words: inducible nitric oxide synthase • C/EBP-ß • hypoxia • gene regulation • pulmonary hypertension
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