Published online before print April 3, 2008, doi: 10.1161/CIRCRESAHA.107.163907
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Submitted on September 10, 2007
Revised on March 14, 2008
Accepted on March 21, 2008
An Acyltransferase Controls the Generation of Hematopoietic and Endothelial Lineages in Zebrafish
Jing-Wei Xiong *;
From the Nephrology Division (J.-W.X., Q.Y., J.Z.) and Cardiovascular Research Center (J.-W.X., J.D.M.), Massachusetts General Hospital and Harvard Medical School, Charlestown, Mass.
* To whom correspondence should be addressed. E-mail: Xiong{at}cvrc.mgh.harvard.edu.
Hematopoietic and endothelial cells develop from a common progenitor, the hemangioblast, or directly from mesodermal cells. The molecular pathway that regulates the specification of both cell lineages remains elusive. Here, we show that a lysocardiolipin acyltransferase, lycat, is critical for the establishment of both hematopoietic and endothelial lineages. We isolated lycat from the deletion interval of cloche, a zebrafish mutant that has dramatically reduced hematopoietic and endothelial cell lineages. Reduction of lycat mRNA levels in wild-type zebrafish embryos decreases both endothelial and hematopoietic lineages. lycat mRNA rescues blood lineages in zebrafish cloche mutant embryos. E165R and G166L mutations in the highly conserved catalytic domain in Lycat abolish its function in zebrafish hematopoiesis. Epistasis analysis supports that lycat acts upstream of scl and etsrp in zebrafish hemangioblast development. These data indicate that lycat is the earliest known player in the generation of both endothelial and hematopoietic lineages.
Key words: acyltransferase • hemangioblast • hematopoiesis • vasculogenesis • zebrafish • cloche
Related Article:
- Lycat and cloche at the Switch Between Blood Vessel Growth and Differentiation?
- Sybill Patan
Circ. Res. 2008 102: 1005-1007.[Extract] [Full Text] [PDF]
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