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(Circulation Research. 2004;95:e8.)
© 2004 American Heart Association, Inc.

Letter to the Editor

Red Blood Cell-Mediated Hypoxic Vasodilatation

A Balanced Physiological Viewpoint

Philip E. James, Timothy Tufnell-Barret, Alex B. Milsom, Michael P. Frenneaux

Department of Cardiology, Wales Heart Research Institute, University of Wales College of Medicine, Heath Park, Cardiff, Wales, UK, Jamespp@Cardiff.ac.uk

Derek Lang

Department of Pharmacology, Therapeutics and Toxicology, Wales Heart Research Institute, University of Wales College of Medicine, Heath Park, Cardiff, Wales, UK

An extract of the first 250 words of the full text is provided, because this article has no abstract.

To the Editor:

We were disappointed to read the editorial presented by Drs Gladwin and Schechter,¹ which appeared in the same issue of Circulation Research as our article.² We believe that the views reflected a selective bias from 1 of 2 camps that have posed diametrically opposite views. This editorial was simply another review on nitrite by these authors. We are not members of 1 "camp" or of the other, but simply wish to present our observations in the hope that more physiological data may provide clarification.

Although Gladwin and Schechter began by stating that the purpose of their editorial was not to discuss our article, they proceeded to criticize our work throughout the editorial. Here, we wish to set the record straight on several important scientific issues that we believe were misrepresented in the Gladwin and Schechter editorial. Specifically,

1. They make a lengthy point of questioning the levels of nitrosyl hemoglobin (HbNO) we describe and how this might be reflected in vivo and advise follow-up studies with electron paramagnetic resonance (EPR). We do not believe that EPR can add to our results, other than by further confirming HbNO production. EPR is capable of selectively measuring accurately only 5 coordinate HbNO hyperfine signals. With the use of appropriate control spectra from 5 and 6 coordinate purified HbNO and a poorly resolved ßHbNO, complicated spectral simulation and subtraction yields qualitative information on these subunits/species in a human blood sample at best. Importantly, the suggested studies will not influence the correlation we . . . [Full Text of this Article]

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