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(Circulation Research. 2004;95:92.)
© 2004 American Heart Association, Inc.

Integrative Physiology

Embryonic Heart Failure in NFATc1^–/– Mice

Novel Mechanistic Insights From In Utero Ultrasound Biomicroscopy

Colin K.L. Phoon, Rui Ping Ji, Orlando Aristizábal, Diane M. Worrad, Bin Zhou, H. Scott Baldwin, Daniel H. Turnbull

From the Pediatric Cardiology Program (C.K.L.P.), Skirball Institute of Biomolecular Medicine (C.K.L.P., R.P.J., O.A., D.H.T.), and Departments of Radiology and Pathology (D.H.T.), New York University School of Medicine, New York, NY; Division of Cardiothoracic Surgery (D.M.W.), Children’s Hospital of Philadelphia, Philadelphia, Pa; and Division of Pediatric Cardiology (B.Z., H.S.B.), Vanderbilt University School of Medicine, Nashville, Tenn.

Correspondence to Colin K.L. Phoon, MPhil, MD, Pediatric Cardiology Program, 530 First Ave, FPT Suite 9U, New York, NY 10016. E-mail colin.phoon{at}med.nyu.edu

Gene targeting in the mouse has become a standard approach, yielding important new insights into the genetic factors underlying cardiovascular development and disease. However, we still have very limited understanding of how mutations affect developing cardiovascular function, and few studies have been performed to measure altered physiological parameters in mouse mutant embryos. Indeed, although in utero lethality due to embryonic heart failure is one of the most common results of gene targeting experiments in the mouse, the underlying physiological mechanisms responsible for embryonic demise remain elusive. Using in utero ultrasound biomicroscopy (UBM), we studied embryonic day (E) 10.5 to 14.5 NFATc1^–/– embryos and control littermates. NFATc1^–/– mice, which lack outflow valves, die at mid-late gestation from presumed defects in forward blood flow with resultant heart failure. UBM showed increasing abnormal regurgitant flow in the aorta and extending into the embryonal–placental circulation, which was evident after E12.5 when outflow valves normally first develop. Reduced NFATc1^–/– net volume flow and diastolic dysfunction contributed to heart failure, but contractile function remained unexpectedly normal. Among 107 NFATc1^–/– embryos imaged, only 2 were observed to be in acute decline with progressive bradyarrhythmia, indicating that heart failure occurs rapidly in individual NFATc1^–/– embryos. This study is among the first linking a specific physiological phenotype with a defined genotype, and demonstrates that NFATc1^–/– embryonic heart failure is a complex phenomenon not simply attributable to contractile dysfunction.

Key Words: cardiac development • embryonic circulation • heart failure • NFAT • ultrasound biomicroscopy

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