doi: 10.1161/01.RES.0000125647.56687.D3
© 2004 American Heart Association, Inc.
Editorials |
Conductive Bridges in Cardiac Tissue
A Beneficial Role or an Arrhythmogenic Substrate?
From the Cardiac Bioelectricity Research and Training Center, Case Western Reserve University, Cleveland, Ohio.
Correspondence to Yoram Rudy, PhD, Director, Cardiac Bioelectricity Research and Training Center, Case Western Reserve University, Cleveland, OH 44106-7207. E-mail yxr{at}cwru.edu
Key Words: fibroblasts • electrophysiology • gap junctions • fibrosis • arrhythmias
For many years, propagation of excitation in cardiac tissue was considered to occur in a homogeneous electrical syncytium. This view has permitted the application of concepts and quantitative relationships developed originally by Hodgkin and Huxley to describe action-potential propagation in the continuous structure of the nerve axon.1 The properties of action-potential conduction were associated with membrane properties alone, while effects of the myocardial architecture were, for the most part, ignored. The microscopic anatomical structure of the myocardium as an assembly of discrete cells separated by a periodic intercalated disk structure was established in the 1950s, followed in later years by the identification of gap junctions as specialized structures for electrical communication between cardiac cells.2–5 The pioneering work of Madison Spach and coworkers in the early 1980s6,7 directed attention to the effects of structural discontinuities on action-potential propagation in the heart. Spach’s experiments revealed electrical properties of cardiac tissue that could not be explained on the basis of the theory that describes conduction of excitation in a continuous medium (the so-called continuous cable theory). For example, the maximum rate of action-potential depolarization, (dV/dt)max, was observed to increase as propagation velocity decreased with wider angles of propagation relative to the myocardial fiber axis. This inverse relationship between (dV/dt)max and velocity is in sharp contrast to the direct relation between these parameters, which is a hallmark of conduction in continuous structures. Thus, conduction in cardiac tissue is discontinuous and its properties are influenced profoundly by the myocardial architecture. The theory of discontinuous conduction predicts the experimentally observed differences from continuous conduction and provides a mechanistic explanation for these differences in terms of the interplay between membrane factors and structural factors during action-potential propagation.8,9
In recent years, increasing attention has been given not only to the discontinuities of myocardial architecture, but also to inhomogeneities in its structure and their effects on cardiac electrical activity. Such inhomogeneities include regional differences of ion-channel expression,10,11 localization of ion channels to subcellular structures of the myocyte,12–14 and differences in the molecular structure of gap junctions (different connexin isoforms) in different cardiac tissues.15
The article by Camelliti et al in this issue of Circulation Research16 draws attention to another form of structural heterogeneity, the presence of nonmyocytes in cardiac tissue, as a possible modulator of action-potential conduction in the heart. About half of the cells in the normal heart are noncardiomyocytes, with fibroblasts being the predominant cell type.17,18 Fibroblasts synthesize the scaffolding structure that supports cardiomyocytes. In aged, hypertrophied, or infarcted hearts, fibroblasts produce electrically insulating collagenous septa. Camelliti et al explore the possibility that fibroblasts form functional gap junctions and communicate electrically with other fibroblasts and with cardiomyocytes in native cardiac tissue (rabbit sinoatrial node). Using confocal laser-scanning microscopy and immunohistochemical techniques to study structure, and spread of Lucifer yellow dye to evaluate the functionality of intercellular coupling, they arrive at the following conclusions. (1) Fibroblasts express both Cx40 and Cx45 to form functional gap junctions. (2) Cx40 is found primarily in regions where fibroblasts are surrounded by other fibroblasts, while Cx45 is expressed mostly where fibroblasts intermingle with myocytes. (3) Gap junctions formed by Cx40 provide fibroblast–fibroblast coupling, while heterogeneous fibroblast-myocyte coupling is provided mostly by the Cx45 isoform. (4) Cx43 is not expressed in nodal tissue from the central region of the sinus node but provides myocyte-myocyte coupling in atrial fibers that protrude into this region. Importantly, the dye-spread studies suggest that fibroblasts can provide conductive pathways between myocytes that are not in direct contact, thus forming bridges for electrical communication.
The study of Camelliti et al16 suggests the possibility of electrical bridging by fibroblasts in native cardiac tissue. An earlier study by Gaudesius et al19 demonstrated such a phenomenon in a patterned cell culture, where strands of myocytes were bridged by chains of cardiac fibroblasts over various distances. Using multisite optical recording of the transmembrane voltage, action-potential propagation in this preparation was documented. Bridging of impulse propagation by fibroblast inserts was successful over distances up to 300 µm, with long propagation delays ranging from 1 to 68 ms introduced by the fibroblast discontinuities (the delay increased with the length of the fibroblast chain). The electrotonic nature of the transmission across the fibroblasts was supported by excluding mechanical stretch as a possible mechanism (replacement of fibroblasts by communication-deficient HeLa cells stopped conduction, while HeLa cells expressing Cx43 permitted conduction over distances of 600 µm).
The principles and properties of conduction in a system containing a segment (bridge) of inexcitable tissue were studied in the 1980s in a series of elegant experiments using three-compartment preparations, where the middle segment was made inexcitable by perfusion with ion-free sucrose or with "ischemic" solutions containing high concentration of potassium.20–24 In these experiments, various phenomena that are potentially arrhythmogenic were observed in Purkinje fibers and later in atrial and ventricular tissues. Observations included long conduction delays across the inexcitable gap, rate-dependent conduction block patterns, electrotonic facilitation and inhibition of conduction, and reflected reentry. These principles and characteristics of conduction apply, also, to conductive bridges formed by nonmyocytes in native cardiac tissue, as suggested by the studies of Camelliti et al16 and Gaudesius et al.19
Propagation of excitation in cardiac tissue is determined by the balance between the availability of depolarizing charge (source) and the amount of charge required for successful propagation (sink). This relationship reflects complex interactions between membrane ionic currents that generate the depolarizing charge and structural properties of the tissue that determine the electric load on a depolarizing cell. Both membrane factors and structural factors can be affected by pathology. Inexcitable bridges constitute an electrical load that, under certain conditions, could tilt the balance in the negative direction, causing conduction failure. Excessive electrical loading can also draw depolarizing charge during the plateau phase of an action potential, leading to location-dependent shortening of action potential duration and arrhythmogenic excitability gradients ("dispersion of repolarization"). The long conduction delays across such bridges can support very slow conduction, which, together with large dispersion of repolarization, provides the substrate for reentrant arrhythmias. On the other hand, fibroblast bridges could have a beneficial effect by providing a mechanism for electrical communication and synchronization across inexcitable barriers in the heart. Such barriers are formed by collagenous septa and scars in fibrotic hearts in association with aging, myocardial infarction, and other forms of structural heart disease. It will be of great interest to establish whether fibroblast bridges form functional gap junctions with cardiomyocytes in such substrates, and if so, to understand their beneficial effects and arrhythmogenic potential in these settings.
The article by Camelliti et al16 describes fibroblast networks in the sinoatrial node. As stated above, it will be of interest and of important clinical relevance to conduct similar studies in other regions of the heart. The sinoatrial node is a highly heterogeneous structure. Nodal cells rely on a multiplicity of ion channels for pacemaking, and these ion channels are expressed heterogeneously within the sinus node structure.25 An article by Vinogradova et al,26 also in this issue of Circulation Research, suggests that rhythmic calcium release also participates in the pacemaking function of sinoatrial nodal cells, an observation that further complicates structure-function relationships in the sinus node. In addition, the heterogeneous architecture of the sinoatrial node (heterogeneous distribution of gap junctions, branching of fibers)25 serves to optimize its electrical loading by the surrounding atrial tissue, thereby facilitating its ability to drive the heart. The work of Camelliti et al suggests that fibroblast bridges exist in the operational sinoatrial node. The role of such bridges is not known. One possibility is that they provide synchronization to this complex heterogeneous structure. Understanding of their functional importance and arrhythmic implications in the sinoatrial node and other tissues of the heart will require further extensive investigation.
Footnotes
The opinions expressed in this editorial are not necessarily those of the editors or of the American Heart Association.
References
- Hodgkin A, Huxley A. A quantitative description of membrane current and its application to excitation and conduction in nerve. J Physiol. 1952; 117: 500–544.
[Free Full Text] - Barr L, Dewey MM, Berger W. Propagation of action potentials and the nexus in cardiac muscle. J Gen Physiol. 1965; 48: 797–823.
[Abstract/Free Full Text] - Weidmann S. The functional significance of the intercalated disks. In: Taccardi B, Marchetti G, eds. Electrophysiology of the Heart. Oxford, UK: Pergamon; 1965: 149–157.
- Loewenstein WR. Junctional intercellular communication: the cell-to-cell membrane channel. Physiol Rev. 1981; 61: 829–913.
[Free Full Text] - DeMello WC. Cell-to-cell communication in heart and other tissues. Prog Biophys Mol Biol. 1982; 39: 147–182.[Medline] [Order article via Infotrieve]
- Spach MS, Miller WT III, Geselowitz DB, Barr RC, Kootsey JM, Johnson EA. The discontinuous nature of propagation in normal canine cardiac muscle: evidence for recurrent discontinuities of intracellular resistance that affect the membrane currents. Circ Res. 1981; 48: 39–54.
[Free Full Text] - Spach M, Kootsey JM, Sloan JD. Active modulation of electrical coupling between cardiac cells of the dog: a mechanism for transient and steady state variations in conduction velocity. Circ Res. 1982; 51: 347–362.
[Abstract/Free Full Text] - Rudy Y, Quan WL. A model study of the effects of the discrete cellular structure on electrical propagation in cardiac tissue. Circ Res. 1987; 61: 815–823.
[Abstract/Free Full Text] - Shaw RM, Rudy Y. Ionic mechanisms of propagation in cardiac tissue: roles of the sodium and L-type calcium currents during reduced excitability and decreased gap junction coupling. Circ Res. 1997; 81: 727–741.
[Abstract/Free Full Text] - Nerbonne JM. Molecular analysis of voltage-gated K+ channel diversity and functioning in the mammalian heart. In: The Handbook of Physiology. The Cardiovascular System. The Heart. Section 2, Vol I. Bethesda, Md: American Physiological Society; 2002: 568–594.
- Antzelevitch C, Dumaine R. Electrical heterogeneity in the heart: physiological, pharmacological and clinical implications. In: The Handbook of Physiology. The Cardiovascular System. The Heart. Section 2, Vol I. Bethesda, Md: American Physiological Society; 2002: 654–692.
- Cohen SA. Immunocytochemical localization of rH1 sodium channel in adult rat heart atria and ventricle: presence in terminal intercalated discs. Circulation. 1994; 94: 3083–3086.
- Kucera JP, Rohr S, Rudy Y. Localization of sodium channels in intercalated disks modulates cardiac conduction. Circ Res. 2002; 91: 1176–1182.
[Abstract/Free Full Text] - Gathercole D, Colling D, Skepper J, Takagishi Y, Levi A, Severs N. Immunogold-labeled L-type calcium channels are clustered in the surface plasma membrane overlying junctional sarcoplasmic reticulum in guinea-pig myocytes: implications for excitation-contraction coupling in cardiac muscle. J Mol Cell Cardiol. 2000; 32: 1981–1994.[CrossRef][Medline] [Order article via Infotrieve]
- Kanter HL, Laing JG, Beau SL, Beyer EC, Saffitz JE. Distinct patterns of connexin expression in canine Purkinje fibers and ventricular muscle. Circ Res. 1993; 72: 1124–1131.
[Abstract/Free Full Text] - Camelliti P, Green CR, LeGrice I, Kohl P. Fibroblast network in rabbit sinoatrial node: structural and functional identification of homogeneous and heterogeneous cell coupling. Circ Res. 2004; 94: 828–835.
[Abstract/Free Full Text] - Nag AC. Study of non-muscle cells of the adult mammalian heart: a fine structural analysis and distribution. Cytobios. 1980; 28: 41–61.[Medline] [Order article via Infotrieve]
- Manabe I, Takayuki S, Nagai R. Gene expression in fibroblasts and fibrosis. Circ Res. 2002; 91: 1103–1113.
[Abstract/Free Full Text] - Gaudesius G, Miragoli M, Thomas SP, Rohr S. Coupling of cardiac electrical activity over extended distances by fibroblasts of cardiac origin. Circ Res. 2003; 93: 421–428.
[Abstract/Free Full Text] - Jalife J, Moe GK. Excitation, conduction, and reflection of impulses in isolated bovine and canine cardiac Purkinje fibers. Circ Res. 1981; 42: 233–247.
- Antzelevitch C, Moe GK. Electrotonic inhibition and summation of impulse conduction in mammalian Purkinje fibers. Am J Physiol. 1983; 245: H42–H53.[Medline] [Order article via Infotrieve]
- Rozanski GJ, Jalife J, Moe GK. Reflected reentry in nonhomogeneous ventricular muscle as a mechanism of cardiac arrhythmias. Circulation. 1984; 69: 163–173.
[Abstract/Free Full Text] - Davidenko JM, Antzelevitch C. Electrophysiological mechanisms underlying rate-dependent changes of refractoriness in normal and segmentally depressed canine Purkinje fibers: the characteristics of post-repolarization refractoriness. Circ Res. 1986; 53: 257–268.
- Lukas A, Antzelevitch C. Reflected reentry, delayed conduction, and electrotonic inhibition in segmentally depressed atrial tissues. Can J Physiol Pharmacol. 1989; 67: 757–764.[Medline] [Order article via Infotrieve]
- Boyett MR, Honjo H, Kodama I. The sinoatrial node, a heterogeneous pacemaker structure. Cardiovasc Res. 2000; 47: 658–687.
[Abstract/Free Full Text] - Vinogradova TM, Zhou YY, Maltsev V, Lyashkov A, Stern M, Lakatta EG. Rhythmic ryanodine receptor Ca2+ releases during diastolic depolarization of sinoatrial pacemaker cells do not require membrane depolarization. Circ Res. 2004; 94: 802–809.
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