Circulation Research. 2003;92:1098-1106
Published online before print April 24, 2003, doi: 10.1161/01.RES.0000073584.46059.E3
Published online before print April 24, 2003, doi: 10.1161/01.RES.0000073584.46059.E3
© 2003 American Heart Association, Inc.
Molecular Medicine |
VEGF-D Is the Strongest Angiogenic and Lymphangiogenic Effector Among VEGFs Delivered Into Skeletal Muscle via Adenoviruses
From the Department of Biotechnology and Molecular Medicine (T.T.R., J.E.M., M.G., T.H., A.P., I.K., S.Y.-H.), A.I. Virtanen Institute, Kuopio University, Finland; National NMR facility (M.I.K., R.A.K.), A.I. Virtanen Institute, Kuopio University, Finland; Ludwig Institute for Cancer Research (M.G.A., S.A.S.), Royal Melbourne Hospital, Victoria, Australia; and the Molecular/Cancer Biology Laboratory (K.A.), Haartman Institute, University of Helsinki, Finland.
Correspondence to Seppo Ylä-Herttuala, MD, PhD, Department of Biotechnology and Molecular Medicine, A.I. Virtanen Institute, University of Kuopio, PO Box 1627, FIN-70211 Kuopio, Finland. E-mail Seppo.Ylaherttuala{at}uku.fi
Optimal angiogenic and lymphangiogenic gene therapy requires knowledge of the best growth factors for each purpose. We studied the therapeutic potential of human vascular endothelial growth factor (VEGF) family members VEGF-A, VEGF-B, VEGF-C, and VEGF-D as well as a VEGFR-3–specific mutant (VEGF-C156S) using adenoviral gene transfer in rabbit hindlimb skeletal muscle. The significance of proteolytic processing of VEGF-D was explored using adenoviruses encoding either full-length or mature (
NC) VEGF-D. Adenoviruses expressing potent VEGFR-2 ligands, VEGF-A and VEGF-DNC, induced the strongest angiogenesis and vascular permeability effects as assessed by capillary vessel and perfusion measurements, modified Miles assay, and MRI. The most significant feature of angiogenesis induced by both VEGF-A and VEGF-DNC was a remarkable enlargement of microvessels with efficient recruitment of pericytes suggesting formation of arterioles or venules. VEGF-A also moderately increased capillary density and created glomeruloid bodies, clusters of tortuous vessels, whereas VEGF-DNC–induced angiogenesis was more diffuse. Vascular smooth muscle cell proliferation occurred in regions with increased plasma protein extravasation, indicating that arteriogenesis may be promoted by VEGF-A and VEGF-DNC. Full-length VEGF-C and VEGF-D induced predominantly and the selective VEGFR-3 ligand VEGF-C156S exclusively lymphangiogenesis. Unlike angiogenesis, lymphangiogenesis was not dependent on nitric oxide. The VEGFR-1 ligand VEGF-B did not promote either angiogenesis or lymphangiogenesis. Finally, we found a positive correlation between capillary size and vascular permeability. This study compares, for the first time, angiogenesis and lymphangiogenesis induced by gene transfer of different human VEGFs, and shows that VEGF-D is the most potent member when delivered via an adenoviral vector into skeletal muscle.
Key Words: vascular permeability • magnetic resonance imaging • edema • pericyte • arteriogenesis
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|
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 |
|
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|
|
|
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|
|
|
|
 |
|
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|
|
|
|
|
|
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|
|
|
|
 |
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
 |
|
 R. R. Foster, S. C. Slater, J. Seckley, D. Kerjaschki, D. O. Bates, P. W. Mathieson, and S. C. Satchell Vascular Endothelial Growth Factor-C, a Potential Paracrine Regulator of Glomerular Permeability, Increases Glomerular Endothelial Cell Monolayer Integrity and Intracellular Calcium Am. J. Pathol., October 1, 2008; 173(4): 938 - 948. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Li, M. Tjwa, I. Van Hove, B. Enholm, E. Neven, K. Paavonen, M. Jeltsch, T. D. Juan, R. E. Sievers, E. Chorianopoulos, et al. Reevaluation of the Role of VEGF-B Suggests a Restricted Role in the Revascularization of the Ischemic Myocardium Arterioscler Thromb Vasc Biol, September 1, 2008; 28(9): 1614 - 1620. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Karpanen and K. Alitalo VEGF-D: a modifier of embryonic lymphangiogenesis Blood, September 1, 2008; 112(5): 1547 - 1548. [Full Text] [PDF]
|
|
|
|
|
|
A. Ny, M. Koch, W. Vandevelde, M. Schneider, C. Fischer, A. Diez-Juan, E. Neven, I. Geudens, S. Maity, L. Moons, et al. Role of VEGF-D and VEGFR-3 in developmental lymphangiogenesis, a chemicogenetic study in Xenopus tadpoles Blood, September 1, 2008; 112(5): 1740 - 1749. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
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|
|
|
|
 |
|
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|
|
|
|
|
|
N. Mochizuki and Y.-G. Kwon 15-Lipoxygenase-1 in the Vasculature: Expanding Roles in Angiogenesis Circ. Res., February 1, 2008; 102(2): 143 - 145. [Full Text] [PDF]
|
|
|
|
|
|
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|
|
|
|
 |
|
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|
|
|
|
|
|
H. Koyama, N. Kobayashi, M. Harada, M. Takeoka, Y. Kawai, K. Sano, M. Fujimori, J. Amano, T. Ohhashi, R. Kannagi, et al. Significance of Tumor-Associated Stroma in Promotion of Intratumoral Lymphangiogenesis: Pivotal Role of a Hyaluronan-Rich Tumor Microenvironment Am. J. Pathol., January 1, 2008; 172(1): 179 - 193. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
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|
|
|
|
 |
|
 R. Kivela, H. Kyrolainen, H. Selanne, P. V. Komi, H. Kainulainen, and V. Vihko A single bout of exercise with high mechanical loading induces the expression of Cyr61/CCN1 and CTGF/CCN2 in human skeletal muscle J Appl Physiol, October 1, 2007; 103(4): 1395 - 1401. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. S. Sundar and T. S. Ganesan Role of Lymphangiogenesis in Cancer J. Clin. Oncol., September 20, 2007; 25(27): 4298 - 4307. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
T. Tammela, Y. He, J. Lyytikka, M. Jeltsch, J. Markkanen, K. Pajusola, S. Yla-Herttuala, and K. Alitalo Distinct Architecture of Lymphatic Vessels Induced by Chimeric Vascular Endothelial Growth Factor-C/Vascular Endothelial Growth Factor Heparin-Binding Domain Fusion Proteins Circ. Res., May 25, 2007; 100(10): 1468 - 1475. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Goldman, K. A. Conley, A. Raehl, D. M. Bondy, B. Pytowski, M. A. Swartz, J. M. Rutkowski, D. B. Jaroch, and E. L. Ongstad Regulation of lymphatic capillary regeneration by interstitial flow in skin Am J Physiol Heart Circ Physiol, May 1, 2007; 292(5): H2176 - H2183. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. K. McColl, K. Paavonen, T. Karnezis, N. C. Harris, N. Davydova, J. Rothacker, E. C. Nice, K. W. Harder, S. Roufail, M. L. Hibbs, et al. Proprotein convertases promote processing of VEGF-D, a critical step for binding the angiogenic receptor VEGFR-2 FASEB J, April 1, 2007; 21(4): 1088 - 1098. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Kopfstein, T. Veikkola, V. G. Djonov, V. Baeriswyl, T. Schomber, K. Strittmatter, S. A. Stacker, M. G. Achen, K. Alitalo, and G. Christofori Distinct Roles of Vascular Endothelial Growth Factor-D in Lymphangiogenesis and Metastasis Am. J. Pathol., April 1, 2007; 170(4): 1348 - 1361. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Yla-Herttuala, T. T. Rissanen, I. Vajanto, and J. Hartikainen Vascular Endothelial Growth Factors: Biology and Current Status of Clinical Applications in Cardiovascular Medicine J. Am. Coll. Cardiol., March 13, 2007; 49(10): 1015 - 1026. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. von Degenfeld, A. Banfi, M. L. Springer, R. A. Wagner, J. Jacobi, C. R. Ozawa, M. J. Merchant, J. P. Cooke, and H. M. Blau Microenvironmental VEGF distribution is critical for stable and functional vessel growth in ischemia FASEB J, December 1, 2006; 20(14): 2657 - 2659. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Saaristo, T. Tammela, A. Farkkila, M. Karkkainen, E. Suominen, S. Yla-Herttuala, and K. Alitalo Vascular Endothelial Growth Factor-C Accelerates Diabetic Wound Healing Am. J. Pathol., September 1, 2006; 169(3): 1080 - 1087. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Cursiefen, L. Chen, M. Saint-Geniez, P. Hamrah, Y. Jin, S. Rashid, B. Pytowski, K. Persaud, Y. Wu, J. W. Streilein, et al. Nonvascular VEGF receptor 3 expression by corneal epithelium maintains avascularity and vision PNAS, July 25, 2006; 103(30): 11405 - 11410. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Kivela, M. Silvennoinen, A.-M. Touvra, T. M. Lehti, H. Kainulainen, and V. Vihko Effects of experimental type 1 diabetes and exercise training on angiogenic gene expression and capillarization in skeletal muscle FASEB J, July 1, 2006; 20(9): 1570 - 1572. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. R. Foster, S. C. Satchell, J. Seckley, M. S. Emmett, K. Joory, C. Y. Xing, M. A. Saleem, P. W. Mathieson, D. O. Bates, and S. J. Harper VEGF-C promotes survival in podocytes Am J Physiol Renal Physiol, July 1, 2006; 291(1): F196 - F207. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Shibuya, K. Watanabe, H. Yamashita, K. Shimizu, H. Miyashita, M. Abe, T. Moriya, H. Ohta, H. Sonoda, T. Shimosegawa, et al. Isolation and Characterization of Vasohibin-2 as a Homologue of VEGF-Inducible Endothelium-Derived Angiogenesis Inhibitor Vasohibin Arterioscler Thromb Vasc Biol, May 1, 2006; 26(5): 1051 - 1057. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. T. Rissanen, P. Korpisalo, J. E. Markkanen, T. Liimatainen, M.-R. Orden, I. Kholova, A. de Goede, T. Heikura, O. H. Grohn, and S. Yla-Herttuala Blood Flow Remodels Growing Vasculature During Vascular Endothelial Growth Factor Gene Therapy and Determines Between Capillary Arterialization and Sprouting Angiogenesis Circulation, December 20, 2005; 112(25): 3937 - 3946. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Tirziu, K. L. Moodie, Z. W. Zhuang, K. Singer, A. Helisch, J. F. Dunn, W. Li, J. Singh, and M. Simons Delayed Arteriogenesis in Hypercholesterolemic Mice Circulation, October 18, 2005; 112(16): 2501 - 2509. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. W. Mould, S. A. Greco, M. M. Cahill, I. D. Tonks, D. Bellomo, C. Patterson, A. Zournazi, A. Nash, P. Scotney, N. K. Hayward, et al. Transgenic Overexpression of Vascular Endothelial Growth Factor-B Isoforms by Endothelial Cells Potentiates Postnatal Vessel Growth In Vivo and In Vitro Circ. Res., September 16, 2005; 97(6): e60 - e70. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Leppanen, S. Koota, I. Kholova, J. Koponen, C. Fieber, U. Eriksson, K. Alitalo, and S. Yla-Herttuala Gene Transfers of Vascular Endothelial Growth Factor-A, Vascular Endothelial Growth Factor-B, Vascular Endothelial Growth Factor-C, and Vascular Endothelial Growth Factor-D Have No Effects on Atherosclerosis in Hypercholesterolemic Low-Density Lipoprotein-Receptor/Apolipoprotein B48-Deficient Mice Circulation, August 30, 2005; 112(9): 1347 - 1352. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Iwase, N. Nagaya, T. Fujii, T. Itoh, S. Murakami, T. Matsumoto, K. Kangawa, and S. Kitamura Comparison of angiogenic potency between mesenchymal stem cells and mononuclear cells in a rat model of hindlimb ischemia Cardiovasc Res, June 1, 2005; 66(3): 543 - 551. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. E. Baldwin, M. M. Halford, S. Roufail, R. A. Williams, M. L. Hibbs, D. Grail, H. Kubo, S. A. Stacker, and M. G. Achen Vascular Endothelial Growth Factor D Is Dispensable for Development of the Lymphatic System Mol. Cell. Biol., March 15, 2005; 25(6): 2441 - 2449. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. H. Laitinen, K. J. Airenne, V. P. Hytonen, E. Peltomaa, A. J. Mahonen, T. Wirth, M. M. Lind, K. A. Makela, P. I. Toivanen, D. Schenkwein, et al. A multipurpose vector system for the screening of libraries in bacteria, insect and mammalian cells and expression in vivo Nucleic Acids Res., February 24, 2005; 33(4): e42 - e42. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Tammela, B. Enholm, K. Alitalo, and K. Paavonen The biology of vascular endothelial growth factors Cardiovasc Res, February 15, 2005; 65(3): 550 - 563. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. H. Annex and M. Simons Growth factor-induced therapeutic angiogenesis in the heart: protein therapy Cardiovasc Res, February 15, 2005; 65(3): 649 - 655. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. E. Vlahakis, B. A. Young, A. Atakilit, and D. Sheppard The Lymphangiogenic Vascular Endothelial Growth Factors VEGF-C and -D Are Ligands for the Integrin {alpha}9{beta}1 J. Biol. Chem., February 11, 2005; 280(6): 4544 - 4552. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Iwase, N. Nagaya, T. Fujii, T. Itoh, H. Ishibashi-Ueda, M. Yamagishi, K. Miyatake, T. Matsumoto, S. Kitamura, and K. Kangawa Adrenomedullin Enhances Angiogenic Potency of Bone Marrow Transplantation in a Rat Model of Hindlimb Ischemia Circulation, January 25, 2005; 111(3): 356 - 362. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Mura, C. C. dos Santos, D. Stewart, and M. Liu Vascular endothelial growth factor and related molecules in acute lung injury J Appl Physiol, November 1, 2004; 97(5): 1605 - 1617. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Jia, A. Bagherzadeh, R. Bicknell, M. R. Duchen, D. Liu, and I. Zachary Vascular Endothelial Growth Factor (VEGF)-D and VEGF-A Differentially Regulate KDR-mediated Signaling and Biological Function in Vascular Endothelial Cells J. Biol. Chem., August 20, 2004; 279(34): 36148 - 36157. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. van Weel, M. M.L. Deckers, J. M. Grimbergen, K. J.M. van Leuven, J. H.P. Lardenoye, R. O. Schlingemann, G. P. van Nieuw Amerongen, J. H. van Bockel, V. W.M. van Hinsbergh, and P. H.A. Quax Vascular Endothelial Growth Factor Overexpression in Ischemic Skeletal Muscle Enhances Myoglobin Expression In Vivo Circ. Res., July 9, 2004; 95(1): 58 - 66. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Rutanen, T. T. Rissanen, J. E. Markkanen, M. Gruchala, P. Silvennoinen, A. Kivela, A. Hedman, M. Hedman, T. Heikura, M.-R. Orden, et al. Adenoviral Catheter-Mediated Intramyocardial Gene Transfer Using the Mature Form of Vascular Endothelial Growth Factor-D Induces Transmural Angiogenesis in Porcine Heart Circulation, March 2, 2004; 109(8): 1029 - 1035. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Fluck, T. Gustafsson, T. Rissanen, and S. Yla-Herttuala VEGF Protein in Human Ischemic Skeletal Muscle Am. J. Pathol., December 1, 2003; 163(6): 2636 - 2638. [Full Text]
|
|