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(Circulation Research. 2002;91:1056.)
© 2002 American Heart Association, Inc.

Integrative Physiology

Ventricular Hypertrophy Plus Neurohumoral Activation Is Necessary to Alter the Cardiac ß-Adrenoceptor System in Experimental Heart Failure

Kirsten Leineweber, Katja Brandt, Beate Wludyka, Anja Beilfuß, Klaus Pönicke, Ingrid Heinroth-Hoffmann, Otto-Erich Brodde

From the Institute of Pharmacology (K.L., K.B., B.W., A.B., K.P., I.H.-H., O.-E.B.), Martin-Luther-University of Halle-Wittenberg, Halle, Germany; and the Institute of Pathophysiology (K.L., O.-E.B.), University of Essen School of Medicine, Essen, Germany.

Correspondence to Prof Dr Otto-Erich Brodde, Institute of Pathophysiology, University of Essen School of Medicine, Hufelandstr. 55, D-45147 Essen, Germany. E-mail otto-erich.brodde{at}uni-essen.de

Treatment of rats with monocrotaline (MCT) leads to pulmonary hypertension, right ventricular (RV) hypertrophy, and finally to RV heart failure. This is associated with characteristic changes in right ventricular ß-adrenoceptors (ß-AR), neuronal noradrenaline transporter (NAT) density and activity (uptake₁), and G protein–coupled receptor kinase (GRK) activity. This study aimed to find out factors that determine ß-AR, uptake₁, and GRK changes. Thus, 6-week-old rats were treated with 50 mg/kg MCT subcutaneous or 0.9% saline. Within 13 to 19 days after MCT application (group A), RV weight (222±6 versus 147±5 mg) and RV/left ventricular (LV) weight ratio (0.42±0.01 versus 0.29±0.01) were significantly increased, whereas plasma noradrenaline, RV ß-AR density, RV NAT density and activity, and RV GRK activity were not significantly altered. Twenty-one to twenty-eight days after MCT (group B), however, not only RV weight (316±4 versus 148±2 mg) and RV/LV weight ratio (0.61±0.01 versus 0.3±0.01) were markedly increased but also plasma noradrenaline (645±63 versus 278±18 pg/mL); now, RV ß-AR density (13.4±1.3 versus 26.5±1.1 fmol/mg protein), RV NAT density (50.9±11.3 versus 79.6±2.9 fmol/mg protein), and RV NAT activity (65.4±7.4 versus 111.8±15.9 pmol [³H]-NA/mg tissue slices/15 min) were significantly decreased and RV-membrane GRK activity (100±15 versus 67±6 [³²P]-rhodopsin in cpm) significantly increased. LV parameters of MCT-treated rats were only marginally different from control LV. We conclude that in MCT-treated rats ventricular hypertrophy per se is not sufficient to cause characteristic alterations in the myocardial ß-AR system often seen in heart failure; only if ventricular hypertrophy is associated with neurohumoral activation ß-ARs are downregulated and GRK activity is increased.

Key Words: monocrotaline • right heart hypertrophy • neurohumoral activation • ß-adrenoceptor • G protein–coupled receptor kinase

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