Circulation Research. 2002;90:866-873
Published online before print March 28, 2002, doi: 10.1161/01.RES.0000016837.26733.BE
Published online before print March 28, 2002, doi: 10.1161/01.RES.0000016837.26733.BE
© 2002 American Heart Association, Inc.
Molecular Medicine |
Domain Mapping Studies Reveal That the M Domain of hsp90 Serves as a Molecular Scaffold to Regulate Akt-Dependent Phosphorylation of Endothelial Nitric Oxide Synthase and NO Release
From the Department of Pharmacology and Molecular Cardiobiology Program (J.F., D.F., Y.C., T.A.F., T.J.M., W.C.S.), Boyer Center for Molecular Medicine, Yale University School of Medicine, New Haven, Conn; and the Institute of Molecular and Cellular Biosciences (N.F., T.T.), Bunkyo-ku, Tokyo, Japan.
Correspondence to William C. Sessa, PhD, Dept of Pharmacology and Molecular Cardiobiology Program, Boyer Center for Molecular Medicine, Yale University School of Medicine, 295 Congress Ave, New Haven, CT 06536. E-mail william.sessa{at}yale.edu
Protein-protein interactions with the molecular chaperone hsp90 and phosphorylation on serine 1179 by the protein kinase Akt leads to activation of endothelial nitric oxide synthase. However, the interplay between these protein-protein interactions remains to be established. In the present study, we show that vascular endothelial growth factor stimulates the coordinated association of hsp90, Akt, and resultant phosphorylation of eNOS. Characterization of the domains of hsp90 required to bind eNOS, using yeast 2-hybrid, cell-based coprecipitation experiments, and GST-fusion proteins, revealed that the M region of hsp90 interacts with the amino terminus of eNOS and Akt. The addition of purified hsp90 to in vitro kinase assays facilitates Akt-driven phosphorylation of recombinant eNOS protein, but not a short peptide encoding the Akt phosphorylation site, suggesting that hsp90 may function as a scaffold for eNOS and Akt. In vivo, coexpression of adenoviral or the cDNA for hsp90 with eNOS promotes nitric oxide release; an effect eliminated using a catalytically functional phosphorylation mutant of eNOS. These results demonstrate that stimulation of endothelial cells with vascular endothelial growth factor recruits eNOS and Akt to an adjacent region on the same domain of hsp90, thereby facilitating eNOS phosphorylation and enzyme activation.
Key Words: nitric oxide • signaling • scaffold • hsp90 • Akt
This article has been cited by other articles:
 |
|
 |
|
  K. Stangl and V. Stangl The ubiquitin proteasome pathway and endothelial (dys)function Cardiovasc Res, October 13, 2009; (2009) cvp315v2. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Musicki, A. E. Ross, H. C. Champion, A. L. Burnett, and T. J. Bivalacqua Posttranslational Modification of Constitutive Nitric Oxide Synthase in the Penis J Androl, July 1, 2009; 30(4): 352 - 362. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-L. Balligand, O. Feron, and C. Dessy eNOS Activation by Physical Forces: From Short-Term Regulation of Contraction to Chronic Remodeling of Cardiovascular Tissues Physiol Rev, April 1, 2009; 89(2): 481 - 534. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. E. Oishi, D. A. Wiseman, S. Sharma, S. Kumar, Y. Hou, S. A. Datar, A. Azakie, M. J. Johengen, C. Harmon, S. Fratz, et al. Progressive dysfunction of nitric oxide synthase in a lamb model of chronically increased pulmonary blood flow: a role for oxidative stress Am J Physiol Lung Cell Mol Physiol, November 1, 2008; 295(5): L756 - L766. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Hu, J. Chen, Q. Wei, and Y. Xia Bidirectional Actions of Hydrogen Peroxide on Endothelial Nitric-oxide Synthase Phosphorylation and Function: CO-COMMITMENT AND INTERPLAY OF Akt AND AMPK J. Biol. Chem., September 12, 2008; 283(37): 25256 - 25263. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Belova, D. R. Brickley, B. Ky, S. K. Sharma, and S. D. Conzen Hsp90 Regulates the Phosphorylation and Activity of Serum- and Glucocorticoid-regulated Kinase-1 J. Biol. Chem., July 4, 2008; 283(27): 18821 - 18831. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. A. Alaamery and C. S. Hoffman Schizosaccharomyces pombe Hsp90/Git10 Is Required for Glucose/cAMP Signaling Genetics, April 1, 2008; 178(4): 1927 - 1936. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K.-J. Yang, S. Shin, L. Piao, E. Shin, Y. Li, K. A. Park, H. S. Byun, M. Won, J. Hong, G. R. Kweon, et al. Regulation of 3-Phosphoinositide-dependent Protein Kinase-1 (PDK1) by Src Involves Tyrosine Phosphorylation of PDK1 and Src Homology 2 Domain Binding J. Biol. Chem., January 18, 2008; 283(3): 1480 - 1491. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Q. Miao, J. Fontana, D. Fulton, M. I. Lin, K. D. Harrison, and W. C. Sessa Dominant-Negative Hsp90 Reduces VEGF-Stimulated Nitric Oxide Release and Migration in Endothelial Cells Arterioscler Thromb Vasc Biol, January 1, 2008; 28(1): 105 - 111. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Sharma, N. Sud, D. A. Wiseman, A. L. Carter, S. Kumar, Y. Hou, T. Rau, J. Wilham, C. Harmon, P. Oishi, et al. Altered carnitine homeostasis is associated with decreased mitochondrial function and altered nitric oxide signaling in lambs with pulmonary hypertension Am J Physiol Lung Cell Mol Physiol, January 1, 2008; 294(1): L46 - L56. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Xu, Y. Shi, J. Wang, D. Jones, D. Weilrauch, R. Ying, B. Wakim, and K. A. Pritchard Jr. A Heat Shock Protein 90 Binding Domain in Endothelial Nitric-oxide Synthase Influences Enzyme Function J. Biol. Chem., December 28, 2007; 282(52): 37567 - 37574. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Blouet, F. Mariotti, V. Mathe, D. Tome, and J.-F. Huneau Nitric Oxide Bioavailability and Not Production Is First Altered During the Onset of Insulin Resistance in Sucrose-Fed Rats Experimental Biology and Medicine, December 1, 2007; 232(11): 1458 - 1464. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Duval, F. Le B uf, J. Huot, and J.-P. Gratton Src-mediated Phosphorylation of Hsp90 in Response to Vascular Endothelial Growth Factor (VEGF) Is Required for VEGF Receptor-2 Signaling to Endothelial NO Synthase Mol. Biol. Cell, November 1, 2007; 18(11): 4659 - 4668. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-L. Frossard, E. Schiffer, B. Cikirikcioglu, J. Bourquin, D. R. Morel, and C. M. Pastor Opposite regulation of endothelial NO synthase by HSP90 and caveolin in liver and lungs of rats with hepatopulmonary syndrome Am J Physiol Gastrointest Liver Physiol, October 1, 2007; 293(4): G864 - G870. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Su, D. Kondrikov, and E. R. Block beta-Actin: A Regulator of NOS-3 Sci. Signal., September 18, 2007; 2007(404): pe52 - pe52. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Derakhshan, G. Hao, and S. S. Gross Balancing reactivity against selectivity: The evolution of protein S-nitrosylation as an effector of cell signaling by nitric oxide Cardiovasc Res, July 15, 2007; 75(2): 210 - 219. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. M. Dudzinski and T. Michel Life history of eNOS: Partners and pathways Cardiovasc Res, July 15, 2007; 75(2): 247 - 260. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. H. Oh, J. K. Woo, Y. D. Yazici, J. N. Myers, W.-Y. Kim, Q. Jin, S. S. Hong, H.-J. Park, Y.-G. Suh, K.-W. Kim, et al. Structural Basis for Depletion of Heat Shock Protein 90 Client Proteins by Deguelin J Natl Cancer Inst, June 20, 2007; 99(12): 949 - 961. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. L. Aschner, S. L. Foster, M. Kaplowitz, Y. Zhang, H. Zeng, and C. D. Fike Heat shock protein 90 modulates endothelial nitric oxide synthase activity and vascular reactivity in the newborn piglet pulmonary circulation Am J Physiol Lung Cell Mol Physiol, June 1, 2007; 292(6): L1515 - L1525. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Edlich, F. Erdmann, F. Jarczowski, M.-C. Moutty, M. Weiwad, and G. Fischer The Bcl-2 Regulator FKBP38-Calmodulin-Ca2+ Is Inhibited by Hsp90 J. Biol. Chem., May 25, 2007; 282(21): 15341 - 15348. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Garcia Blanes, M. Oubaha, Y. Rautureau, and J.-P. Gratton Phosphorylation of Tyrosine 801 of Vascular Endothelial Growth Factor Receptor-2 Is Necessary for Akt-dependent Endothelial Nitric-oxide Synthase Activation and Nitric Oxide Release from Endothelial Cells J. Biol. Chem., April 6, 2007; 282(14): 10660 - 10669. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Hawle, D. Horst, J. P. Bebelman, X. X. Yang, M. Siderius, and S. M. van der Vies Cdc37p Is Required for Stress-Induced High-Osmolarity Glycerol and Protein Kinase C Mitogen-Activated Protein Kinase Pathway Functionality by Interaction with Hog1p and Slt2p (Mpk1p) Eukaryot. Cell, March 1, 2007; 6(3): 521 - 532. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. D. Roberts, J. S. Isenberg, L. A. Ridnour, and D. A. Wink Nitric Oxide and Its Gatekeeper Thrombospondin-1 in Tumor Angiogenesis Clin. Cancer Res., February 1, 2007; 13(3): 795 - 798. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Vercauteren, E. Remy, C. Devaux, B. Dautreaux, J.-P. Henry, F. Bauer, P. Mulder, R. Hooft van Huijsduijnen, A. Bombrun, C. Thuillez, et al. Improvement of Peripheral Endothelial Dysfunction by Protein Tyrosine Phosphatase Inhibitors in Heart Failure Circulation, December 5, 2006; 114(23): 2498 - 2507. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Hawle, M. Siepmann, A. Harst, M. Siderius, H. P. Reusch, and W. M. J. Obermann The Middle Domain of Hsp90 Acts as a Discriminator between Different Types of Client Proteins Mol. Cell. Biol., November 15, 2006; 26(22): 8385 - 8395. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Chen, M. Medhora, J. R. Falck, K. A. Pritchard Jr, and E. R. Jacobs Mechanisms of activation of eNOS by 20-HETE and VEGF in bovine pulmonary artery endothelial cells Am J Physiol Lung Cell Mol Physiol, September 1, 2006; 291(3): L378 - L385. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Wei and Y. Xia Proteasome Inhibition Down-regulates Endothelial Nitric-oxide Synthase Phosphorylation and Function J. Biol. Chem., August 4, 2006; 281(31): 21652 - 21659. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Cale and I. M. Bird Dissociation of endothelial nitric oxide synthase phosphorylation and activity in uterine artery endothelial cells Am J Physiol Heart Circ Physiol, April 1, 2006; 290(4): H1433 - H1445. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Yang and X.-F. Ming Recent advances in understanding endothelial dysfunction in atherosclerosis. Clin. Med. Res., March 1, 2006; 4(1): 53 - 65. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. L. Bilodeau and H. E. Hamm Endothelial Nitric-Oxide Synthase Reveals a New Face in G Protein Signaling Mol. Pharmacol., March 1, 2006; 69(3): 677 - 679. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. B. Harris, M. Bartoli, S. G. Sood, R. L. Matts, and R. C. Venema Direct Interaction of the Cell Division Cycle 37 Homolog Inhibits Endothelial Nitric Oxide Synthase Activity Circ. Res., February 17, 2006; 98(3): 335 - 341. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Cohen-Saidon, I. Carmi, A. Keren, and E. Razin Antiapoptotic function of Bcl-2 in mast cells is dependent on its association with heat shock protein 90beta Blood, February 15, 2006; 107(4): 1413 - 1420. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Mata-Greenwood, C. Jenkins, K. N. Farrow, G. G. Konduri, J. A. Russell, S. Lakshminrusimha, S. M. Black, and R. H. Steinhorn eNOS function is developmentally regulated: uncoupling of eNOS occurs postnatally Am J Physiol Lung Cell Mol Physiol, February 1, 2006; 290(2): L232 - L241. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. E. Church and D. Fulton Differences in eNOS Activity Because of Subcellular Localization Are Dictated by Phosphorylation State Rather than the Local Calcium Environment J. Biol. Chem., January 20, 2006; 281(3): 1477 - 1488. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Papapetropoulos, Z. Zhou, C. Gerassimou, G. Yetik, R. C. Venema, C. Roussos, W. C. Sessa, and J. D. Catravas Interaction between the 90-kDa Heat Shock Protein and Soluble Guanylyl Cyclase: Physiological Significance and Mapping of the Domains Mediating Binding Mol. Pharmacol., October 1, 2005; 68(4): 1133 - 1141. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-Y. Han, S. H. Oh, F. Morgillo, J. N. Myers, E. Kim, W. K. Hong, and H.-Y. Lee Hypoxia-inducible Factor 1{alpha} and Antiangiogenic Activity of Farnesyltransferase Inhibitor SCH66336 in Human Aerodigestive Tract Cancer J Natl Cancer Inst, September 7, 2005; 97(17): 1272 - 1286. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Polikandriotis, L. J. Mazzella, H. L. Rupnow, and C. M. Hart Peroxisome Proliferator-Activated Receptor {gamma} Ligands Stimulate Endothelial Nitric Oxide Production Through Distinct Peroxisome Proliferator-Activated Receptor {gamma}-Dependent Mechanisms Arterioscler Thromb Vasc Biol, September 1, 2005; 25(9): 1810 - 1816. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Carrasco-Martin, S. Alonso-Orgaz, J. C De la Pinta, M. Marques, C. Macaya, A. Barrientos, M. M Gonzalez, A. Garcia-Mendez, P. J. Mateos-Caceres, J. C Porres, et al. Endothelial hypoxic preconditioning in rat hypoxic isolated aortic segments Exp Physiol, July 1, 2005; 90(4): 557 - 569. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Martinez-Ruiz, L. Villanueva, C. G. de Orduna, D. Lopez-Ferrer, M. A. Higueras, C. Tarin, I. Rodriguez-Crespo, J. Vazquez, and S. Lamas S-nitrosylation of Hsp90 promotes the inhibition of its ATPase and endothelial nitric oxide synthase regulatory activities PNAS, June 14, 2005; 102(24): 8525 - 8530. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Herrera and J. L. Garvin Recent Advances in the Regulation of Nitric Oxide in the Kidney Hypertension, June 1, 2005; 45(6): 1062 - 1067. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Wei and Y. Xia Roles of 3-Phosphoinositide-dependent Kinase 1 in the Regulation of Endothelial Nitric-oxide Synthase Phosphorylation and Function by Heat Shock Protein 90 J. Biol. Chem., May 6, 2005; 280(18): 18081 - 18086. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Cale, S. C. Tsoi, M. Toppe, M. A. Grummer, M. Ochiai, R. R. Magness, and I. M. Bird Molecular Cloning of Ovine Endothelial Nitric Oxide Synthase and Expression in COS-7 Cells Reproductive Sciences, April 1, 2005; 12(3): 156 - 168. [Abstract] [PDF]
|
|
|
|
 |
|
 T. J. Stalker, Y. Gong, and R. Scalia The Calcium-Dependent Protease Calpain Causes Endothelial Dysfunction in Type 2 Diabetes Diabetes, April 1, 2005; 54(4): 1132 - 1140. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Terasawa, M. Minami, and Y. Minami Constantly Updated Knowledge of Hsp90 J. Biochem., April 1, 2005; 137(4): 443 - 447. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Connelly, M. Madhani, and A. J. Hobbs Resistance to Endotoxic Shock in Endothelial Nitric-oxide Synthase (eNOS) Knock-out Mice: A PRO-INFLAMMATORY ROLE FOR eNOS-DERIVED NO IN VIVO J. Biol. Chem., March 18, 2005; 280(11): 10040 - 10046. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Bucci, F. Roviezzo, I. Posadas, J. Yu, L. Parente, W. C. Sessa, L. J. Ignarro, and G. Cirino Endothelial nitric oxide synthase activation is critical for vascular leakage during acute inflammation in vivo PNAS, January 18, 2005; 102(3): 904 - 908. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Fulton, M. B. Harris, B. E. Kemp, R. C. Venema, M. B. Marrero, and D. W. Stepp Insulin resistance does not diminish eNOS expression, phosphorylation, or binding to HSP-90 Am J Physiol Heart Circ Physiol, December 1, 2004; 287(6): H2384 - H2393. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Kupatt, C. Dessy, R. Hinkel, P. Raake, G. Daneau, C. Bouzin, P. Boekstegers, and O. Feron Heat Shock Protein 90 Transfection Reduces Ischemia-Reperfusion-Induced Myocardial Dysfunction via Reciprocal Endothelial NO Synthase Serine 1177 Phosphorylation and Threonine 495 Dephosphorylation Arterioscler Thromb Vasc Biol, August 1, 2004; 24(8): 1435 - 1441. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. A. Ortiz, N. J. Hong, and J. L. Garvin Luminal flow induces eNOS activation and translocation in the rat thick ascending limb. II. Role of PI3-kinase and Hsp90 Am J Physiol Renal Physiol, August 1, 2004; 287(2): F281 - F288. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Iaccarino, M. Ciccarelli, D. Sorriento, E. Cipolletta, V. Cerullo, G. L. Iovino, A. Paudice, A. Elia, G. Santulli, A. Campanile, et al. AKT Participates in Endothelial Dysfunction in Hypertension Circulation, June 1, 2004; 109(21): 2587 - 2593. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Gidalevitz, C. Biswas, H. Ding, D. Schneidman-Duhovny, H. J. Wolfson, F. Stevens, S. Radford, and Y. Argon Identification of the N-terminal Peptide Binding Site of Glucose-regulated Protein 94 J. Biol. Chem., April 16, 2004; 279(16): 16543 - 16552. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. A. Pritchard Jr., J. Ou, Z. Ou, Y. Shi, J. P. Franciosi, P. Signorino, S. Kaul, C. Ackland-Berglund, K. Witte, S. Holzhauer, et al. Hypoxia-induced acute lung injury in murine models of sickle cell disease Am J Physiol Lung Cell Mol Physiol, April 1, 2004; 286(4): L705 - L714. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Papapetropoulos, D. Fulton, M. I. Lin, J. Fontana, T. J. McCabe, S. Zoellner, G. Garcia-Cardena, Z. Zhou, J.-P. Gratton, and W. C. Sessa Vanadate Is a Potent Activator of Endothelial Nitric-Oxide Synthase: Evidence for the Role of the Serine/Threonine Kinase Akt and the 90-kDa Heat Shock Protein Mol. Pharmacol., February 1, 2004; 65(2): 407 - 415. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Ou, J. T. Fontana, Z. Ou, D. W. Jones, A. W. Ackerman, K. T. Oldham, J. Yu, W. C. Sessa, and K. A. Pritchard Jr. Heat shock protein 90 and tyrosine kinase regulate eNOS NO{middle dot} generation but not NO{middle dot} bioactivity Am J Physiol Heart Circ Physiol, February 1, 2004; 286(2): H561 - H569. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Luo and J. L. Benovic G Protein-coupled Receptor Kinase Interaction with Hsp90 Mediates Kinase Maturation J. Biol. Chem., December 19, 2003; 278(51): 50908 - 50914. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Jiang, D. Cyr, R. W. Babbitt, W. C. Sessa, and C. Patterson Chaperone-dependent Regulation of Endothelial Nitric-oxide Synthase Intracellular Trafficking by the Co-chaperone/Ubiquitin Ligase CHIP J. Biol. Chem., December 5, 2003; 278(49): 49332 - 49341. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. I. Lin, D. Fulton, R. Babbitt, I. Fleming, R. Busse, K. A. Pritchard Jr., and W. C. Sessa Phosphorylation of Threonine 497 in Endothelial Nitric-oxide Synthase Coordinates the Coupling of L-Arginine Metabolism to Efficient Nitric Oxide Production J. Biol. Chem., November 7, 2003; 278(45): 44719 - 44726. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Yoshida and Y. Xia Heat Shock Protein 90 as an Endogenous Protein Enhancer of Inducible Nitric-oxide Synthase J. Biol. Chem., September 19, 2003; 278(38): 36953 - 36958. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Takahashi and M. E. Mendelsohn Synergistic Activation of Endothelial Nitric-oxide Synthase (eNOS) by HSP90 and Akt: CALCIUM-INDEPENDENT eNOS ACTIVATION INVOLVES FORMATION OF AN HSP90-Akt-CaM-BOUND eNOS COMPLEX J. Biol. Chem., August 15, 2003; 278(33): 30821 - 30827. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. C. Kone, T. Kuncewicz, W. Zhang, and Z.-Y. Yu Protein interactions with nitric oxide synthases: controlling the right time, the right place, and the right amount of nitric oxide Am J Physiol Renal Physiol, August 1, 2003; 285(2): F178 - F190. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. C. Venema, V. J. Venema, H. Ju, M. B. Harris, C. Snead, T. Jilling, C. Dimitropoulou, M. E. Maragoudakis, and J. D. Catravas Novel complexes of guanylate cyclase with heat shock protein 90 and nitric oxide synthase Am J Physiol Heart Circ Physiol, July 11, 2003; 285(2): H669 - H678. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. G. Konduri, J. Ou, Y. Shi, and K. A. Pritchard Jr. Decreased association of HSP90 impairs endothelial nitric oxide synthase in fetal lambs with persistent pulmonary hypertension Am J Physiol Heart Circ Physiol, June 5, 2003; 285(1): H204 - H211. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. B. Harris, M. A. Blackstone, H. Ju, V. J. Venema, and R. C. Venema Heat-induced increases in endothelial NO synthase expression and activity and endothelial NO release Am J Physiol Heart Circ Physiol, June 5, 2003; 285(1): H333 - H340. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Duval, S. Bedard-Goulet, C. Delisle, and J.-P. Gratton Vascular Endothelial Growth Factor-dependent Down-regulation of Flk-1/KDR Involves Cbl-mediated Ubiquitination: CONSEQUENCES ON NITRIC OXIDE PRODUCTION FROM ENDOTHELIAL CELLS J. Biol. Chem., May 23, 2003; 278(22): 20091 - 20097. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. P. Lotz, H. Lin, A. Harst, and W. M. J. Obermann Aha1 Binds to the Middle Domain of Hsp90, Contributes to Client Protein Activation, and Stimulates the ATPase Activity of the Molecular Chaperone J. Biol. Chem., May 2, 2003; 278(19): 17228 - 17235. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. M. Bauer, D. Fulton, Y. C. Boo, G. P. Sorescu, B. E. Kemp, H. Jo, and W. C. Sessa Compensatory Phosphorylation and Protein-Protein Interactions Revealed by Loss of Function and Gain of Function Mutants of Multiple Serine Phosphorylation Sites in Endothelial Nitric-oxide Synthase J. Biol. Chem., April 18, 2003; 278(17): 14841 - 14849. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. R. Thom, D. Fisher, J. Zhang, V. M. Bhopale, S. T. Ohnishi, Y. Kotake, T. Ohnishi, and D. G. Buerk Stimulation of perivascular nitric oxide synthesis by oxygen Am J Physiol Heart Circ Physiol, April 1, 2003; 284(4): H1230 - H1239. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Ou, J. Ou, A. W. Ackerman, K. T. Oldham, and K. A. Pritchard Jr L-4F, an Apolipoprotein A-1 Mimetic, Restores Nitric Oxide and Superoxide Anion Balance in Low-Density Lipoprotein-Treated Endothelial Cells Circulation, March 25, 2003; 107(11): 1520 - 1524. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Takahashi and M. E. Mendelsohn Calmodulin-dependent and -independent Activation of Endothelial Nitric-oxide Synthase by Heat Shock Protein 90 J. Biol. Chem., March 7, 2003; 278(11): 9339 - 9344. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Fleming and R. Busse Molecular mechanisms involved in the regulation of the endothelial nitric oxide synthase Am J Physiol Regulatory Integrative Comp Physiol, January 1, 2003; 284(1): R1 - R12. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. I. Nedvetsky, W. C. Sessa, and H. H. H. W. Schmidt There's NO binding like NOS binding: Protein-protein interactions in NO/cGMP signaling PNAS, December 24, 2002; 99(26): 16510 - 16512. [Full Text] [PDF]
|
|
|
|
|
|
K. J. Ho and J. K. Liao Nonnuclear Actions of Estrogen Arterioscler Thromb Vasc Biol, December 1, 2002; 22(12): 1952 - 1961. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Patterson and D. Cyr Welcome to the Machine: A Cardiologist's Introduction to Protein Folding and Degradation Circulation, November 19, 2002; 106(21): 2741 - 2746. [Full Text] [PDF]
|
|
|
|
|
|
A. D. Basso, D. B. Solit, G. Chiosis, B. Giri, P. Tsichlis, and N. Rosen Akt Forms an Intracellular Complex with Heat Shock Protein 90 (Hsp90) and Cdc37 and Is Destabilized by Inhibitors of Hsp90 Function J. Biol. Chem., October 11, 2002; 277(42): 39858 - 39866. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. A. Waheed and T. L. Z. Jones Hsp90 Interactions and Acylation Target the G Protein Galpha 12 but Not Galpha 13 to Lipid Rafts J. Biol. Chem., August 30, 2002; 277(36): 32409 - 32412. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Shi, J. E. Baker, C. Zhang, J. S. Tweddell, J. Su, and K. A. Pritchard Jr Chronic Hypoxia Increases Endothelial Nitric Oxide Synthase Generation of Nitric Oxide by Increasing Heat Shock Protein 90 Association and Serine Phosphorylation Circ. Res., August 23, 2002; 91(4): 300 - 306. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. J. Ho and J. K. Liao Non-nuclear Actions of Estrogen: New Targets for Prevention and Treatment of Cardiovascular Disease Mol. Interv., July 1, 2002; 2(4): 219 - 228. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Shiojima and K. Walsh Role of Akt Signaling in Vascular Homeostasis and Angiogenesis Circ. Res., June 28, 2002; 90(12): 1243 - 1250. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-L. Balligand Heat Shock Protein 90 in Endothelial Nitric Oxide Synthase Signaling: Following the Lead(er)? Circ. Res., May 3, 2002; 90(8): 838 - 841. [Full Text] [PDF]
|
|