Published online before print February 21, 2002, doi: 10.1161/01.RES.0000013700.98464.8E
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
© 2002 American Heart Association, Inc.
Cellular Biology |
Nucleotide Receptors Involved in UTP-Induced Rat Arterial Smooth Muscle Cell Migration
From INSERM U441, Pessac, France.
Correspondence to Alain-Pierre Gadeau, Avenue du Haut Lévêque, 33600 Pessac, France. E-mail alain.gadeau{at}bordeaux.inserm.fr
Many factors have been shown to be involved in the development of hyperplasic lesions of vessels, but the role of extracellular nucleotides remains largely unknown. The presence of P2Y and P2X nucleotide receptors on arterial endothelial and smooth muscle cells suggests a potential role for nucleotides in the vessel pathophysiology. Although the role of P2X in physiology of vessels is well documented, that of P2Y is not completely understood. We recently demonstrated that extracellular nucleotides, and particularly UTP, induced migration of cultured arterial smooth muscle cells (ASMCs). This migration is dependent on osteopontin expression and involves the Rho and mitogen-activated protein (MAP) kinase pathways. An important question is to determine the specific role of the different P2Y receptors of rat ASMCs in the UTP-induced migration process. Therefore, we first quantified mRNA levels of P2Y2, P2Y4, and P2Y6 nucleotide receptors in cultured rat ASMCs by a competitive RT-PCR approach and demonstrated that P2Y2 is the most highly expressed among these receptors potentially involved in the UTP-mediated response. In addition to UTP, UDP also induced ASMC migration even when UTP regeneration was inhibited, suggesting the involvement of UDP receptor P2Y6. Moreover, suramin, a specific antagonist of rat P2Y2 receptor, acted as an inhibitor of UTP-induced migration. Taken together, these results suggest a prominent role for the UTP receptor, P2Y2, and for the UDP receptor, P2Y6, in UTP-induced rat ASMC migration.
Key Words: purinergic receptors • migration • UTP • smooth muscle cells
This article has been cited by other articles:
 |
|
 |
|
  J. Shen and P. E. DiCorleto ADP Stimulates Human Endothelial Cell Migration via P2Y1 Nucleotide Receptor-Mediated Mitogen-Activated Protein Kinase Pathways Circ. Res., February 29, 2008; 102(4): 448 - 456. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Taboubi, J. Milanini, E. Delamarre, F. Parat, F. Garrouste, G. Pommier, J. Takasaki, J.-C. Hubaud, H. Kovacic, and M. Lehmann G{alpha}(q/11)-coupled P2Y2 nucleotide receptor inhibits human keratinocyte spreading and migration FASEB J, December 1, 2007; 21(14): 4047 - 4058. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Jalvy, M.-A. Renault, L. Lam Shang Leen, I. Belloc, A. Reynaud, A.-P. Gadeau, and C. Desgranges CREB Mediates UTP-Directed Arterial Smooth Muscle Cell Migration and Expression of the Chemotactic Protein Osteopontin via Its Interaction with Activator Protein-1 Sites Circ. Res., May 11, 2007; 100(9): 1292 - 1299. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. D. Douillet, W. P. Robinson III, P. M. Milano, R. C. Boucher, and P. B. Rich Nucleotides induce IL-6 release from human airway epithelia via P2Y2 and p38 MAPK-dependent pathways Am J Physiol Lung Cell Mol Physiol, October 1, 2006; 291(4): L734 - L746. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. P. Abbracchio, G. Burnstock, J.-M. Boeynaems, E. A. Barnard, J. L. Boyer, C. Kennedy, G. E. Knight, M. Fumagalli, C. Gachet, K. A. Jacobson, et al. International Union of Pharmacology LVIII: Update on the P2Y G Protein-Coupled Nucleotide Receptors: From Molecular Mechanisms and Pathophysiology to Therapy Pharmacol. Rev., September 1, 2006; 58(3): 281 - 341. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A.-K. Wihlborg, J. Balogh, L. Wang, C. Borna, Y. Dou, B. V. Joshi, E. Lazarowski, K. A. Jacobson, A. Arner, and D. Erlinge Positive Inotropic Effects by Uridine Triphosphate (UTP) and Uridine Diphosphate (UDP) via P2Y2 and P2Y6 Receptors on Cardiomyocytes and Release of UTP in Man During Myocardial Infarction Circ. Res., April 14, 2006; 98(7): 970 - 976. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M.-A. Renault, S. Jalvy, M. Potier, I. Belloc, E. Genot, L. V. Dekker, C. Desgranges, and A.-P. Gadeau UTP Induces Osteopontin Expression through a Coordinate Action of NF{kappa}B, Activator Protein-1, and Upstream Stimulatory Factor in Arterial Smooth Muscle Cells J. Biol. Chem., January 28, 2005; 280(4): 2708 - 2713. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Shen, C. I. Seye, M. Wang, G. A. Weisman, P. A. Wilden, and M. Sturek Cloning, Up-Regulation, and Mitogenic Role of Porcine P2Y2 Receptor in Coronary Artery Smooth Muscle Cells Mol. Pharmacol., November 1, 2004; 66(5): 1265 - 1274. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A.-K. Wihlborg, L. Wang, O. O. Braun, A. Eyjolfsson, R. Gustafsson, T. Gudbjartsson, and D. Erlinge ADP Receptor P2Y12 Is Expressed in Vascular Smooth Muscle Cells and Stimulates Contraction in Human Blood Vessels Arterioscler Thromb Vasc Biol, October 1, 2004; 24(10): 1810 - 1815. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. K. Hogarth, N. Sandbo, S. Taurin, V. Kolenko, J. M. Miano, and N. O. Dulin Dual role of PKA in phenotypic modulation of vascular smooth muscle cells by extracellular ATP Am J Physiol Cell Physiol, August 1, 2004; 287(2): C449 - C456. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.-A. Renault, S. Jalvy, I. Belloc, S. Pasquet, S. Sena, M. Olive, C. Desgranges, and A.-P. Gadeau AP-1 Is Involved in UTP-Induced Osteopontin Expression in Arterial Smooth Muscle Cells Circ. Res., October 3, 2003; 93(7): 674 - 681. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. I. Seye, Q. Kong, L. Erb, R. C. Garrad, B. Krugh, M. Wang, J. T. Turner, M. Sturek, F. A. Gonzalez, and G. A. Weisman Functional P2Y2 Nucleotide Receptors Mediate Uridine 5'-Triphosphate-Induced Intimal Hyperplasia in Collared Rabbit Carotid Arteries Circulation, November 19, 2002; 106(21): 2720 - 2726. [Abstract] [Full Text] [PDF]
|
|