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(Circulation Research. 2002;90:1147.)
© 2002 American Heart Association, Inc.

Reports

Tyrosine Kinase Activity of Discoidin Domain Receptor 1 Is Necessary for Smooth Muscle Cell Migration and Matrix Metalloproteinase Expression

Guangpei Hou, Wolfgang F. Vogel, Michelle P. Bendeck

From the Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario, Canada.

Correspondence to Michelle P. Bendeck, Department of Laboratory Medicine and Pathobiology, University of Toronto, Medical Sciences Building, Room 6217, 1 King’s College Circle, Toronto, Ontario, Canada M5S 1A8. E-mail michelle.bendeck{at}utoronto.ca

Abstract

Smooth muscle cell (SMC) interactions with collagen mediate cell migration during the pathogenesis of atherosclerosis and restenosis. Discoidin domain receptors (DDRs) have been identified as novel collagen receptors. We used aortic SMCs from wild-type and DDR1^-/- mice to evaluate the function of the DDR1 in regulating migration. DDR1^-/- SMCs exhibited impaired attachment to and migration toward a type I collagen substrate. Matrix metalloproteinase-2 (MMP-2) and MMP-9 activities were concomitantly reduced in these cells. Transfection of a full-length cDNA for DDR1b rescued these deficits, whereas kinase-dead mutants of DDR1 restored attachment but not migration and MMP production. These results suggest that active DDR1 kinase is a central mediator of SMC migration.

Key Words: matrix metalloproteinases • smooth muscle cell migration • discoidin domain receptors

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