© 2000 American Heart Association, Inc.
Molecular Medicine |
A Comparison of Aorta and Vena Cava Medial Message Expression by cDNA Array Analysis Identifies a Set of 68 Consistently Differentially Expressed Genes, All in Aortic Media
From the Department of Pathology (L.D.A., S.M.S.), University of Washington, Seattle, Wash; Department of Surgery (R.L.G.), Wake Forest University School of Medicine, Winston-Salem, NC; and Department of Pathology and Laboratory Medicine (B.M.), St. Paul’s Hospital, University of British Columbia, Vancouver, British Columbia, Canada.
Correspondence to Lawrence D. Adams, Department of Pathology, University of Washington, Box 357335, 1959 NE Pacific St, Seattle, WA 98195-7335. E-mail ladams{at}u.washington.edu
Abstract—We performed a
systematic analysis of gene expression in arteries and veins by
comparing message profiles of macaque aorta and vena cava media using a
cDNA array containing 4048 known human genes, 
35% of currently
named human genes (11 000). The data show extensive differences in
RNA expression in artery versus vein media. Sixty-eight genes had
consistent elevation in message expression by the aorta, but
none were elevated in the vena cava. The most differentially expressed
gene was regulator of G-protein signaling (RGS) 5, at an expression
ratio of 46.5±12.6 (mean±SEM). The data set also contained 2 genes
already known to be expressed in the aorta, elastin at 5.0±1.4, and
the aortic preferentially expressed gene 1 (APEG-1) at 2.3±0.6. We
chose to analyze RGS5 expression further because of its high
level of differential expression in the aorta. Levels of RGS5 mRNA were
confirmed by Northern analysis and in situ hybridization. A
human tissue RNA dot blot showed that RGS5 message is highest in aorta,
followed by small intestine, stomach, and then heart. Northern
analysis confirmed that RGS5 expression in human aorta is
higher than in any region of the heart. RGS5 is a G-protein signaling
regulator of unknown specificity most homologous to RGS4, an
inhibitory regulator of pressure-induced cardiac
hypertrophy. The expression pattern of the 68 differential
genes as a whole is a start toward identifying the molecular
phenotypes of arteries and veins on a systematic basis.
Key Words: cDNA array • aorta • vena cava • expression profile • RGS5
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