© 2000 American Heart Association, Inc.
Molecular Medicine |
Host Gene Regulation During Coxsackievirus B3 Infection in Mice
Assessment by Microarrays
From the Cardiovascular Research Laboratory (L.A.T., C.M.C., D.Y., K.S., D.W., B.M.M.), Department of Pathology and Laboratory Medicine, University of British Columbia, St. Paul’s Hospital, Vancouver, Canada, and SCIOS, Inc (G.S., L.W.S.), Sunnyvale, Calif.
Correspondence to Bruce McManus, MD, PhD, Department of Pathology and Laboratory Medicine, University of British Columbia, St. Paul’s Hospital, 1081 Burrard St, Vancouver, BC, Canada V6Z 1Y6. E-mail mcmanus{at}interchange.ubc.ca
Abstract—Host genetic responses that characterize enteroviral myocarditis have not yet been determined. The injurious and inflammatory process in heart muscle may reflect host responses of benefit to the virus and ultimately result in congestive heart failure and dilated cardiomyopathy. On the other hand, host responses within the myocardium may secure the host against acute or protracted damage. To investigate the nature of modified gene expression in comparison with normal tissue, mRNA species were assessed in myocardium using cDNA microarray technology at days 3, 9, and 30 after infection. Of 7000 clones initially screened, 169 known genes had a level of expression significantly different at 1 or more postinfection time points as compared with baseline. The known regulated genes were sorted according to their functional groups and normalized expression patterns and, subsequently, interpreted in the context of viremic, inflammatory, and healing phases of the myocarditic process.
Key Words: coxsackieviruses • gene regulation • microarrays • myocardium
This article has been cited by other articles:
 |
|
 |
|
  L. Venteo, T. Bourlet, F. Renois, F. Douche-Aourik, J.-F. Mosnier, G. Lorain De la Grand Maison, M. Pluot, B. Pozzetto, and L. Andreoletti Enterovirus-related activation of the cardiomyocyte mitochondrial apoptotic pathway in patients with acute myocarditis Eur. Heart J., November 19, 2009; (2009) ehp489v1. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Tsukamoto, T. Minamino, and M. Kitakaze Functional alterations of cardiac proteasomes under physiological and pathological conditions Cardiovasc Res, September 4, 2009; (2009) cvp282v2. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Luo, J. Wong, and B. Wong Protein degradation systems in viral myocarditis leading to dilated cardiomyopathy Cardiovasc Res, August 6, 2009; (2009) cvp225v2. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Yuan, Z. Liu, T. Lim, H. Zhang, J. He, E. Walker, C. Shier, Y. Wang, Y. Su, A. Sall, et al. CXCL10 Inhibits Viral Replication Through Recruitment of Natural Killer Cells in Coxsackievirus B3-Induced Myocarditis Circ. Res., March 13, 2009; 104(5): 628 - 638. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. T. Lua, M. Yasuike, I. Hirono, and T. Aoki Transcription Program of Red Sea Bream Iridovirus as Revealed by DNA Microarrays J. Virol., December 15, 2005; 79(24): 15151 - 15164. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. M. Zhang, J. Yuan, P. Cheung, D. Chau, B. W. Wong, B. M. McManus, and D. Yang Gamma Interferon-Inducible Protein 10 Induces HeLa Cell Apoptosis through a p53-Dependent Pathway Initiated by Suppression of Human Papillomavirus Type 18 E6 and E7 Expression Mol. Cell. Biol., July 15, 2005; 25(14): 6247 - 6258. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-M. Kim, J.-H. Park, S.-K. Chung, J.-Y. Kim, H.-Y. Hwang, K.-C. Chung, I. Jo, S.-I. Park, and J.-H. Nam Coxsackievirus B3 Infection Induces cyr61 Activation via JNK To Mediate Cell Death J. Virol., December 15, 2004; 78(24): 13479 - 13488. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Shen, W. Xu, Y.-W. Chu, Y. Wang, Q.-S. Liu, and S.-D. Xiong Coxsackievirus Group B Type 3 Infection Upregulates Expression of Monocyte Chemoattractant Protein 1 in Cardiac Myocytes, Which Leads to Enhanced Migration of Mononuclear Cells in Viral Myocarditis J. Virol., November 15, 2004; 78(22): 12548 - 12556. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. V. Podgoreanu and D. A. Schwinn Genomics and the circulation Br. J. Anaesth., July 1, 2004; 93(1): 140 - 148. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. W Mason Myocarditis and dilated cardiomyopathy: An inflammatory link Cardiovasc Res, October 15, 2003; 60(1): 5 - 10. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. M. Zhang, J. Yuan, P. Cheung, H. Luo, B. Yanagawa, D. Chau, N. Stephan-Tozy, B. W. Wong, J. Zhang, J. E. Wilson, et al. Overexpression of Interferon-{gamma}-inducible GTPase Inhibits Coxsackievirus B3-induced Apoptosis through the Activation of the Phosphatidylinositol 3-Kinase/Akt Pathway and Inhibition of Viral Replication J. Biol. Chem., August 29, 2003; 278(35): 33011 - 33019. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C Napoli, L O Lerman, V Sica, A Lerman, G Tajana, and F de Nigris Microarray analysis: a novel research tool for cardiovascular scientists and physicians Heart, June 1, 2003; 89(6): 597 - 604. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Luo, J. Zhang, F. Dastvan, B. Yanagawa, M. A. Reidy, H. M. Zhang, D. Yang, J. E. Wilson, and B. M. McManus Ubiquitin-Dependent Proteolysis of Cyclin D1 Is Associated with Coxsackievirus-Induced Cell Growth Arrest J. Virol., December 6, 2002; 77(1): 1 - 9. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Yin, A.-K. Berg, T. Tuvemo, and G. Frisk Enterovirus RNA Is Found in Peripheral Blood Mononuclear Cells in a Majority of Type 1 Diabetic Children at Onset Diabetes, June 1, 2002; 51(6): 1964 - 1971. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. D. Barrans, P. D. Allen, D. Stamatiou, V. J. Dzau, and C.-C. Liew Global Gene Expression Profiling of End-Stage Dilated Cardiomyopathy Using a Human Cardiovascular-Based cDNA Microarray Am. J. Pathol., June 1, 2002; 160(6): 2035 - 2043. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Bolt, K. Berg, and M. Blixenkrone-Moller Measles virus-induced modulation of host-cell gene expression J. Gen. Virol., May 1, 2002; 83(5): 1157 - 1165. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Luo, B. Yanagawa, J. Zhang, Z. Luo, M. Zhang, M. Esfandiarei, C. Carthy, J. E. Wilson, D. Yang, and B. M. McManus Coxsackievirus B3 Replication Is Reduced by Inhibition of the Extracellular Signal-Regulated Kinase (ERK) Signaling Pathway J. Virol., March 7, 2002; 76(7): 3365 - 3373. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. V. de Avalos, I. J. Blader, M. Fisher, J. C. Boothroyd, and B. A. Burleigh Immediate/Early Response to Trypanosoma cruzi Infection Involves Minimal Modulation of Host Cell Transcription J. Biol. Chem., January 4, 2002; 277(1): 639 - 644. [Abstract] [Full Text]
|
|
|
|
 |
|
 S. Ehrt, D. Schnappinger, S. Bekiranov, J. Drenkow, S. Shi, T. R. Gingeras, T. Gaasterland, G. Schoolnik, and C. Nathan Reprogramming of the Macrophage Transcriptome in Response to Interferon-{gamma} and Mycobacterium tuberculosis: Signaling Roles of Nitric Oxide Synthase-2 and Phagocyte Oxidase J. Exp. Med., October 15, 2001; 194(8): 1123 - 1140. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. P. Liu and J. W. Mason Advances in the Understanding of Myocarditis Circulation, August 28, 2001; 104(9): 1076 - 1082. [Full Text] [PDF]
|
|
|
|
|
|
J. S. Rao and M. Bond Microarrays : Managing the Data Deluge Circ. Res., June 22, 2001; 88(12): 1226 - 1227. [Full Text] [PDF]
|
|
|
|
 |
|
 B. J. ARONOW, T. TOYOKAWA, A. CANNING, K. HAGHIGHI, U. DELLING, E. KRANIAS, J. D. MOLKENTIN, and G. W. DORN II Divergent transcriptional responses to independent genetic causes of cardiac hypertrophy Physiol Genomics, June 6, 2001; 6(1): 19 - 28. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. K. Geiss, M. C. An, R. E. Bumgarner, E. Hammersmark, D. Cunningham, and M. G. Katze Global Impact of Influenza Virus on Cellular Pathways Is Mediated by both Replication-Dependent and -Independent Events J. Virol., May 1, 2001; 75(9): 4321 - 4331. [Abstract] [Full Text]
|
|
|
|
|
|
I. J. Blader, I. D. Manger, and J. C. Boothroyd Microarray Analysis Reveals Previously Unknown Changes in Toxoplasma gondii-infected Human Cells J. Biol. Chem., June 22, 2001; 276(26): 24223 - 24231. [Abstract] [Full Text] [PDF]
|
|