This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Gittenberger-de Groot, A. C. Articles by Poelmann, R. E. Search for Related Content PubMed PubMed Citation Articles by Gittenberger-de Groot, A. C. Articles by Poelmann, R. E. Related Collections Angiogenesis Animal models of human disease Pediatric and congenital heart disease, including cardiovascular surgery Cardiac development

(Circulation Research. 2000;87:969.)
© 2000 American Heart Association, Inc.

Reports

Epicardial Outgrowth Inhibition Leads to Compensatory Mesothelial Outflow Tract Collar and Abnormal Cardiac Septation and Coronary Formation

Adriana C. Gittenberger-de Groot, Mark-Paul F.M. Vrancken Peeters, Maarten Bergwerff, Monica M.T. Mentink, Robert E. Poelmann

From the Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, the Netherlands.

Correspondence to Prof Dr A.C. Gittenberger-de Groot, Department of Anatomy and Embryology, Leiden University Medical Center, PO Box 9602, 2300 RC Leiden, the Netherlands. E-mail acgitten{at}lumc.nl

In the present study, we investigated the modulatory role of the epicardium in myocardial and coronary development. Epicardial cell tracing experiments have shown that epicardium-derived cells are the source of interstitial myocardial fibroblasts, cushion mesenchyme, and smooth muscle cells. Epicardial outgrowth inhibition studies show abnormalities of the compact myocardial layer, myocardialization of cushion tissue, looping, septation, and coronary vascular formation. Lack of epicardial spreading is partly compensated by mesothelial outgrowth over the conotruncal region. Heterospecific epicardial transplant is able to partially rescue the myocardial development, as well as septation and coronary formation.

Key Words: epicardium • coronary vasculature • myocardial differentiation • ablation • chimerization

This article has been cited by other articles:

				P. Snider, K. N. Standley, J. Wang, M. Azhar, T. Doetschman, and S. J. Conway Origin of Cardiac Fibroblasts and the Role of Periostin Circ. Res., November 6, 2009; 105(10): 934 - 947. [Abstract] [Full Text] [PDF]

				C. Stamm, Y.-H. Choi, B. Nasseri, and R. Hetzer A heart full of stem cells: the spectrum of myocardial progenitor cells in the postnatal heart Therapeutic Advances in Cardiovascular Disease, June 1, 2009; 3(3): 215 - 229. [Abstract] [PDF]

				K. J. Lavine and D. M. Ornitz Shared Circuitry: Developmental Signaling Cascades Regulate Both Embryonic and Adult Coronary Vasculature Circ. Res., January 30, 2009; 104(2): 159 - 169. [Abstract] [Full Text] [PDF]

				R. J. Tomanek, H. K. Hansen, and L. P. Christensen Temporally Expressed PDGF and FGF-2 Regulate Embryonic Coronary Artery Formation and Growth Arterioscler Thromb Vasc Biol, July 1, 2008; 28(7): 1237 - 1243. [Abstract] [Full Text] [PDF]

				D. P. Kolditz, M. C.E.F. Wijffels, N. A. Blom, A. van der Laarse, N. D. Hahurij, H. Lie-Venema, R. R. Markwald, R. E. Poelmann, M. J. Schalij, and A. C. Gittenberger-de Groot Epicardium-Derived Cells in Development of Annulus Fibrosis and Persistence of Accessory Pathways Circulation, March 25, 2008; 117(12): 1508 - 1517. [Abstract] [Full Text] [PDF]

				P. F. van Loo, E. A. F. Mahtab, L. J. Wisse, J. Hou, F. Grosveld, G. Suske, S. Philipsen, and A. C. Gittenberger-de Groot Transcription Factor Sp3 Knockout Mice Display Serious Cardiac Malformations Mol. Cell. Biol., December 15, 2007; 27(24): 8571 - 8582. [Abstract] [Full Text] [PDF]

				Y. Ishii, J. D. Langberg, R. Hurtado, S. Lee, and T. Mikawa Induction of proepicardial marker gene expression by the liver bud Development, October 15, 2007; 134(20): 3627 - 3637. [Abstract] [Full Text] [PDF]

				J. T Butcher and R. R Markwald Valvulogenesis: the moving target Phil Trans R Soc B, August 29, 2007; 362(1484): 1489 - 1503. [Abstract] [Full Text] [PDF]

				E.M. Winter, R.W. Grauss, B. Hogers, J. van Tuyn, R. van der Geest, H. Lie-Venema, R. V. Steijn, S. Maas, M.C. DeRuiter, A.A.F. deVries, et al. Preservation of Left Ventricular Function and Attenuation of Remodeling After Transplantation of Human Epicardium-Derived Cells Into the Infarcted Mouse Heart Circulation, August 21, 2007; 116(8): 917 - 927. [Abstract] [Full Text] [PDF]

				K. J. Lavine, A. C. White, C. Park, C. S. Smith, K. Choi, F. Long, C.-c. Hui, and D. M. Ornitz Fibroblast growth factor signals regulate a wave of Hedgehog activation that is essential for coronary vascular development. Genes & Dev., June 15, 2006; 20(12): 1651 - 1666. [Abstract] [Full Text] [PDF]

				R. P. Visconti, Y. Ebihara, A. C. LaRue, P. A. Fleming, T. C. McQuinn, M. Masuya, H. Minamiguchi, R. R. Markwald, M. Ogawa, and C. J. Drake An In Vivo Analysis of Hematopoietic Stem Cell Potential: Hematopoietic Origin of Cardiac Valve Interstitial Cells Circ. Res., March 17, 2006; 98(5): 690 - 696. [Abstract] [Full Text] [PDF]

				I. Eralp, H. Lie-Venema, M. C. DeRuiter, N. M.S van den Akker, A. J.J.C. Bogers, M. M.T. Mentink, R. E. Poelmann, and A. C. Gittenberger-de Groot Coronary Artery and Orifice Development Is Associated With Proper Timing of Epicardial Outgrowth and Correlated Fas Ligand Associated Apoptosis Patterns Circ. Res., March 18, 2005; 96(5): 526 - 534. [Abstract] [Full Text] [PDF]

				A. J. Watt, M. A. Battle, J. Li, and S. A. Duncan GATA4 is essential for formation of the proepicardium and regulates cardiogenesis PNAS, August 24, 2004; 101(34): 12573 - 12578. [Abstract] [Full Text] [PDF]

				R. W. Stottmann, M. Choi, Y. Mishina, E. N. Meyers, and J. Klingensmith BMP receptor IA is required in mammalian neural crest cells for development of the cardiac outflow tract and ventricular myocardium Development, May 1, 2004; 131(9): 2205 - 2218. [Abstract] [Full Text] [PDF]

				H. Lie-Venema, A. C. Gittenberger-de Groot, L. J.P. van Empel, M. J. Boot, H. Kerkdijk, E. de Kant, and M. C. DeRuiter Ets-1 and Ets-2 Transcription Factors Are Essential for Normal Coronary and Myocardial Development in Chicken Embryos Circ. Res., April 18, 2003; 92(7): 749 - 756. [Abstract] [Full Text] [PDF]

				D. E. Reese, T. Mikawa, and D. M. Bader Development of the Coronary Vessel System Circ. Res., November 1, 2002; 91(9): 761 - 768. [Abstract] [Full Text] [PDF]