© 1999 American Heart Association, Inc.
Molecular Medicine |
Sphingosylphosphorylcholine Induces a Hypertrophic Growth Response Through the Mitogen-Activated Protein Kinase Signaling Cascade in Rat Neonatal Cardiac Myocytes
From the Second Department of Internal Medicine (K.S., T.Y., M.K., R.N.), Gunma University School of Medicine, and Laboratory of Signal Transduction (F.O.), Institute for Molecular and Cellular Regulation, Gunma University School of Medicine, Maebashi, Japan.
Correspondence to Tomoyuki Yokoyama, MD, Second Department of Internal Medicine, Gunma University School of Medicine, 3-39-22, Showa-machi, Maebashi, 371, Japan. E-mail yokoyamt{at}news.sb.gunma-u.ac.jp
Abstract—The sphingolipid metabolites, sphingosine (SPH), SPH 1-phosphate (S1P), and sphingosylphosphorylcholine (SPC), can act as intracellular as well as extracellular signaling molecules. These compounds have been implicated in the regulation of cell growth, differentiation, and programmed cell death in nonmyocytes, but the effects of sphingolipid metabolites in cardiac myocytes are not known. Cultured neonatal rat cardiac myocytes were stimulated with SPH (1 to 10 µmol/L), S1P (1 to 10 µmol/L), or SPC (0.1 to 10 µmol/L) for 24 hours to determine the effects of sphingolipid metabolites on the rates of protein synthesis and degradation. Stimulation with SPC led to an increase in the total amount of protein, an accelerated rate of total protein synthesis, and a decrease in protein degradation in a dose-dependent manner. However, S1P had little effect and SPH had no effect on total protein synthesis. In addition, stimulation with SPC led to a 1.4-fold increase in myocardial cell size and enhanced atrial natriuretic factor gene expression. Pretreatment of the cardiac myocytes with pertussis toxin or PD98059 attenuated the SPC-induced hypertrophic growth response. Further, stimulation with SPC increased phosphorylation of mitogen-activated protein kinase (MAPK) and stimulated MAPK enzyme activity. Finally, endothelin-1 stimulated the generation of SPC in cardiac myocytes. The observation that SPC induces a hypertrophic growth response in cardiac myocytes suggests that SPC may play a critical role in the development of cardiac hypertrophy. The effects of SPC could be mediated, in part, by activation of a G protein–coupled receptor and a MAPK signaling cascade.
Key Words: sphingolipid • cardiac myocyte • hypertrophy • receptor • mitogen-activated protein kinase
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