© 1999 American Heart Association, Inc.
Original Contributions |
Direct Demonstration of P-Selectin– and VCAM-1–Dependent Mononuclear Cell Rolling in Early Atherosclerotic Lesions of Apolipoprotein E–Deficient Mice
From the Departments of Biomedical Engineering (C.L.R., Y.H., U.J., S.G., D.R.M., K.L.) and Internal Medicine (I.J.S.), University of Virginia Health Sciences Center, Charlottesville. The current affiliation for C.L.R. is Southwestern Oklahoma State University, School of Pharmacy, Weatherford, Okla.
Correspondence to Klaus Ley, MD, Department of Biomedical Engineering, University of Virginia, Box 377, Health Sciences Center, Charlottesville, VA 22908. E-mail kfl3f{at}virginia.edu
Abstract—Apolipoprotein
E–deficient (ApoE-/-) mice develop atherosclerotic
lesions throughout the arterial tree, including the carotid
bifurcation. Although the expression of adhesion molecules such as
ICAM-1, vascular cell adhesion molecule-1 (VCAM-1), and P-selectin on
endothelium that overlie atherosclerotic plaques has
been implicated in monocyte recruitment to developing lesions, monocyte
adhesion in atherosclerotic vessels has not been observed directly. To
investigate which adhesion molecules may be important in monocyte
adhesion to atherosclerotic lesions, an isolated mouse carotid artery
preparation was developed and perfused with mononuclear cells. We show
rolling and attachment of the human monocytic cell line U937 and the
mouse monocyte-macrophage cell line P388D1 in carotid arteries
from 10- to 12-week-old ApoE-/- and C57BL/6 wild-type
mice fed a Western-type diet (21% fat wt/wt) for 4 to 5 weeks. No
rolling was observed in carotid arteries from C57BL/6 or BALB/c
wild-type mice fed a chow diet and little was observed in BALB/c mice
fed a Western-type diet. This model represents early lesion
development as shown by minimal macrophage infiltration in the
intima of carotid arteries from ApoE-/- mice fed a
Western-type diet. Rolling was observed at shear stresses that were
characteristic of the low-shear recirculation zone near the carotid
bifurcation. Mononuclear cell attachment and rolling were significantly
inhibited by monoclonal antibody blockade of P-selectin or its
leukocyte ligand P-selectin glycoprotein ligand-1. Rolling
velocities increased after monoclonal antibody blockade of mononuclear
cell 
4-integrin or VCAM-1, which indicates that
4-integrin interacting with VCAM-1 stabilizes rolling
interactions and prolongs monocyte transit times.
Key Words: apolipoprotein • rolling • selectin • monocyte • atherosclerosis
This article has been cited by other articles:
 |
|
 |
|
  R. C. Laxton, Y. Hu, J. Duchene, F. Zhang, Z. Zhang, K.-Y. Leung, Q. Xiao, R. S. Scotland, C. P. Hodgkinson, K. Smith, et al. A Role of Matrix Metalloproteinase-8 in Atherosclerosis Circ. Res., October 23, 2009; 105(9): 921 - 929. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. A. Kaufmann Ultrasound molecular imaging of atherosclerosis Cardiovasc Res, September 1, 2009; 83(4): 617 - 625. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. P. Choudhury and E. A. Fisher Molecular Imaging in Atherosclerosis, Thrombosis, and Vascular Inflammation Arterioscler Thromb Vasc Biol, July 1, 2009; 29(7): 983 - 991. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Kisucka, A. K. Chauhan, B.-Q. Zhao, I. S. Patten, A. Yesilaltay, M. Krieger, and D. D. Wagner Elevated levels of soluble P-selectin in mice alter blood-brain barrier function, exacerbate stroke, and promote atherosclerosis Blood, June 4, 2009; 113(23): 6015 - 6022. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Piconi, D. Trabattoni, C. Luraghi, E. Perilli, M. Borelli, M. Pacei, G. Rizzardini, A. Lattuada, D. H. Bray, M. Catalano, et al. Treatment of periodontal disease results in improvements in endothelial dysfunction and reduction of the carotid intima-media thickness FASEB J, April 1, 2009; 23(4): 1196 - 1204. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. J. Woollard, A. Suhartoyo, E. E. Harris, S. U. Eisenhardt, S. P. Jackson, K. Peter, A. M. Dart, M. J. Hickey, and J. P.F. Chin-Dusting Pathophysiological Levels of Soluble P-Selectin Mediate Adhesion of Leukocytes to the Endothelium Through Mac-1 Activation Circ. Res., November 7, 2008; 103(10): 1128 - 1138. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. An, H. Wang, R. Tang, T. Yago, J. M. McDaniel, S. McGee, Y. Huo, and L. Xia P-Selectin Glycoprotein Ligand-1 Is Highly Expressed on Ly-6Chi Monocytes and a Major Determinant for Ly-6Chi Monocyte Recruitment to Sites of Atherosclerosis in Mice Circulation, June 24, 2008; 117(25): 3227 - 3237. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. McAteer, J. E. Schneider, Z. A. Ali, N. Warrick, C. A. Bursill, C. von zur Muhlen, D. R. Greaves, S. Neubauer, K. M. Channon, and R. P. Choudhury Magnetic Resonance Imaging of Endothelial Adhesion Molecules in Mouse Atherosclerosis Using Dual-Targeted Microparticles of Iron Oxide Arterioscler Thromb Vasc Biol, January 1, 2008; 28(1): 77 - 83. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. E. Vendrov, Z. S. Hakim, N. R. Madamanchi, M. Rojas, C. Madamanchi, and M. S. Runge Atherosclerosis Is Attenuated by Limiting Superoxide Generation in Both Macrophages and Vessel Wall Cells Arterioscler Thromb Vasc Biol, December 1, 2007; 27(12): 2714 - 2721. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Galkina and K. Ley Vascular Adhesion Molecules in Atherosclerosis Arterioscler Thromb Vasc Biol, November 1, 2007; 27(11): 2292 - 2301. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. J. Cho, P. Shashkin, C. A. Gleissner, D. Dunson, N. Jain, J. K. Lee, Y. Miller, and K. Ley Induction of dendritic cell-like phenotype in macrophages during foam cell formation Physiol Genomics, April 24, 2007; 29(2): 149 - 160. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Osaka, S. Hagita, M. Haraguchi, M. Kajimura, M. Suematsu, and M. Yoshida Real-time imaging of mechanically injured femoral artery in mice reveals a biphasic pattern of leukocyte accumulation Am J Physiol Heart Circ Physiol, April 1, 2007; 292(4): H1876 - H1882. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Dever, C. M. Spickett, S. Kennedy, C. Rush, G. Tennant, A. Monopoli, and C. L. Wainwright The Nitric Oxide-Donating Pravastatin Derivative, NCX 6550 [(1S-[1{alpha}(betaS*, {delta}S*), 2{alpha}, 6{alpha}, 8beta-(R*), 8a{alpha}]]-1,2,6,7,8,8a-Hexahydro-beta, {delta}, 6-trihydroxy-2-methyl-8-(2-methyl-1-oxobutoxy)-1-naphtalene-heptanoic Acid 4-(Nitrooxy)butyl Ester)], Reduces Splenocyte Adhesion and Reactive Oxygen Species Generation in Normal and Atherosclerotic Mice J. Pharmacol. Exp. Ther., January 1, 2007; 320(1): 419 - 426. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Kawakami, M. Aikawa, P. Alcaide, F. W. Luscinskas, P. Libby, and F. M. Sacks Apolipoprotein CIII Induces Expression of Vascular Cell Adhesion Molecule-1 in Vascular Endothelial Cells and Increases Adhesion of Monocytic Cells Circulation, August 15, 2006; 114(7): 681 - 687. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. T. Bolick, S. Srinivasan, A. Whetzel, L. C. Fuller, and C. C. Hedrick 12/15 Lipoxygenase Mediates Monocyte Adhesion to Aortic Endothelium in Apolipoprotein E-Deficient Mice Through Activation of RhoA and NF-{kappa}B Arterioscler Thromb Vasc Biol, June 1, 2006; 26(6): 1260 - 1266. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. A. Vorchheimer and R. Becker Platelets in Atherothrombosis Mayo Clin. Proc., January 1, 2006; 81(1): 59 - 68. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. J. Lowenstein Platelets trigger endothelial exocytosis Blood, October 1, 2005; 106(7): 2223 - 2223. [Full Text] [PDF]
|
|
|
|
 |
|
 K. Liu, D. S. Chi, C. Li, H. K. Hall, D. M. Milhorn, and G. Krishnaswamy HIV-1 Tat protein-induced VCAM-1 expression in human pulmonary artery endothelial cells and its signaling Am J Physiol Lung Cell Mol Physiol, August 1, 2005; 289(2): L252 - L260. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Piccio, B. Rossi, L. Colantonio, R. Grenningloh, A. Gho, L. Ottoboni, J. W. Homeister, E. Scarpini, M. Martinello, C. Laudanna, et al. Efficient Recruitment of Lymphocytes in Inflamed Brain Venules Requires Expression of Cutaneous Lymphocyte Antigen and Fucosyltransferase-VII J. Immunol., May 1, 2005; 174(9): 5805 - 5813. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Ostermann, L. Fraemohs, T. Baltus, A. Schober, M. Lietz, A. Zernecke, E. A. Liehn, and C. Weber Involvement of JAM-A in Mononuclear Cell Recruitment on Inflamed or Atherosclerotic Endothelium: Inhibition by Soluble JAM-A Arterioscler Thromb Vasc Biol, April 1, 2005; 25(4): 729 - 735. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Furnkranz, A. Schober, V. N. Bochkov, P. Bashtrykov, G. Kronke, A. Kadl, B. R. Binder, C. Weber, and N. Leitinger Oxidized Phospholipids Trigger Atherogenic Inflammation in Murine Arteries Arterioscler Thromb Vasc Biol, March 1, 2005; 25(3): 633 - 638. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-X. Chen, X. Ma, S. Whitman, and E. R. O'Brien Novel Antiinflammatory Vascular Benefits of Systemic and Stent-Based Delivery of Ethylisopropylamiloride Circulation, December 14, 2004; 110(24): 3721 - 3726. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. W. Homeister, A. Daugherty, and J. B. Lowe {alpha}(1,3)Fucosyltransferases FucT-IV and FucT-VII Control Susceptibility to Atherosclerosis in Apolipoprotein E-/- Mice Arterioscler Thromb Vasc Biol, October 1, 2004; 24(10): 1897 - 1903. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Bhatia, K. Matsushita, M. Yamakuchi, C. N. Morrell, W. Cao, and C. J. Lowenstein Ceramide Triggers Weibel-Palade Body Exocytosis Circ. Res., August 6, 2004; 95(3): 319 - 324. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. M. Rijcken, M. G. Laukoetter, C. Anthoni, S. Meier, R. Mennigen, H.-U. Spiegel, M. Bruewer, N. Senninger, D. Vestweber, and C. F. Krieglstein Immunoblockade of PSGL-1 attenuates established experimental murine colitis by reduction of leukocyte rolling Am J Physiol Gastrointest Liver Physiol, July 1, 2004; 287(1): G115 - G124. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Isoda, S. Sawada, N. Ishigami, T. Matsuki, K. Miyazaki, M. Kusuhara, Y. Iwakura, and F. Ohsuzu Lack of Interleukin-1 Receptor Antagonist Modulates Plaque Composition in Apolipoprotein E-Deficient Mice Arterioscler Thromb Vasc Biol, June 1, 2004; 24(6): 1068 - 1073. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Manka, S. B. Forlow, J. M. Sanders, D. Hurwitz, D. K. Bennett, S. A. Green, K. Ley, and I. J. Sarembock Critical Role of Platelet P-Selectin in the Response to Arterial Injury in Apolipoprotein-E-Deficient Mice Arterioscler Thromb Vasc Biol, June 1, 2004; 24(6): 1124 - 1129. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. Zeiffer, A. Schober, M. Lietz, E. A. Liehn, W. Erl, N. Emans, Z.-q. Yan, and C. Weber Neointimal Smooth Muscle Cells Display a Proinflammatory Phenotype Resulting in Increased Leukocyte Recruitment Mediated by P-Selectin and Chemokines Circ. Res., April 2, 2004; 94(6): 776 - 784. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Cornelissen, J. Armstrong, and C. M. Holt Mechanical Stretch Induces Phosphorylation of p38-MAPK and Apoptosis in Human Saphenous Vein Arterioscler Thromb Vasc Biol, March 1, 2004; 24(3): 451 - 456. [Abstract] [Full Text]
|
|
|
|
|
|
D. D. Wagner and P. C. Burger Platelets in Inflammation and Thrombosis Arterioscler Thromb Vasc Biol, December 1, 2003; 23(12): 2131 - 2137. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Chen, D. B. Jump, M. B. Grant, W. J. Esselman, and J. V. Busik Dyslipidemia, but Not Hyperglycemia, Induces Inflammatory Adhesion Molecules in Human Retinal Vascular Endothelial Cells Invest. Ophthalmol. Vis. Sci., November 1, 2003; 44(11): 5016 - 5022. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. OSTERUD and E. BJORKLID Role of Monocytes in Atherogenesis Physiol Rev, October 1, 2003; 83(4): 1069 - 1112. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Battistini, L. Piccio, B. Rossi, S. Bach, S. Galgani, C. Gasperini, L. Ottoboni, D. Ciabini, M. D. Caramia, G. Bernardi, et al. CD8+ T cells from patients with acute multiple sclerosis display selective increase of adhesiveness in brain venules: a critical role for P-selectin glycoprotein ligand-1 Blood, June 15, 2003; 101(12): 4775 - 4782. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. J. Lyon, R. E. Law, and W. A. Hsueh Minireview: Adiposity, Inflammation, and Atherogenesis Endocrinology, June 1, 2003; 144(6): 2195 - 2200. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. W. Phillips, K. G. Barringhaus, J. M. Sanders, S. E. Hesselbacher, A. C. Czarnik, D. Manka, D. Vestweber, K. Ley, and I. J. Sarembock Single Injection of P-Selectin or P-Selectin Glycoprotein Ligand-1 Monoclonal Antibody Blocks Neointima Formation After Arterial Injury in Apolipoprotein E-Deficient Mice Circulation, May 6, 2003; 107(17): 2244 - 2249. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Kirii, T. Niwa, Y. Yamada, H. Wada, K. Saito, Y. Iwakura, M. Asano, H. Moriwaki, and M. Seishima Lack of Interleukin-1{beta} Decreases the Severity of Atherosclerosis in ApoE-Deficient Mice Arterioscler Thromb Vasc Biol, April 1, 2003; 23(4): 656 - 660. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. C. Burger and D. D. Wagner Platelet P-selectin facilitates atherosclerotic lesion development Blood, April 1, 2003; 101(7): 2661 - 2666. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. H. Von der Thusen, J. Kuiper, T. J. C. Van Berkel, and E. A. L. Biessen Interleukins in Atherosclerosis: Molecular Pathways and Therapeutic Potential Pharmacol. Rev., March 1, 2003; 55(1): 133 - 166. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Massberg, K. Brand, S. Gruner, S. Page, E. Muller, I. Muller, W. Bergmeier, T. Richter, M. Lorenz, I. Konrad, et al. A Critical Role of Platelet Adhesion in the Initiation of Atherosclerotic Lesion Formation J. Exp. Med., October 7, 2002; 196(7): 887 - 896. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E Rijcken, C F Krieglstein, C Anthoni, M G Laukoetter, R Mennigen, H U Spiegel, N Senninger, C F Bennett, and G Schuermann ICAM-1 and VCAM-1 antisense oligonucleotides attenuate in vivo leucocyte adherence and inflammation in rat inflammatory bowel disease Gut, October 1, 2002; 51(4): 529 - 535. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Li, H. Chen, F. Romeo, T. Sawamura, T. Saldeen, and J. L. Mehta Statins Modulate Oxidized Low-Density Lipoprotein-Mediated Adhesion Molecule Expression in Human Coronary Artery Endothelial Cells: Role of LOX-1 J. Pharmacol. Exp. Ther., August 1, 2002; 302(2): 601 - 605. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. M. Lessner, H. L. Prado, E. K. Waller, and Z. S. Galis Atherosclerotic Lesions Grow Through Recruitment and Proliferation of Circulating Monocytes in a Murine Model Am. J. Pathol., June 1, 2002; 160(6): 2145 - 2155. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. E. Rosenfeld Leukocyte Recruitment Into Developing Atherosclerotic Lesions: The Complex Interaction Between Multiple Molecules Keeps Getting More Complex Arterioscler Thromb Vasc Biol, March 1, 2002; 22(3): 361 - 363. [Full Text] [PDF]
|
|
|
|
 |
|
 M. R. King and D. A. Hammer Multiparticle adhesive dynamics: Hydrodynamic recruitment of rolling leukocytes PNAS, December 18, 2001; 98(26): 14919 - 14924. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. K. Hansson Immune Mechanisms in Atherosclerosis Arterioscler Thromb Vasc Biol, December 1, 2001; 21(12): 1876 - 1890. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. K. Stannard, D. R. Riddell, S. M. Sacre, A. D. Tagalakis, C. Langer, A. von Eckardstein, P. Cullen, T. Athanasopoulos, G. Dickson, and J. S. Owen Cell-derived Apolipoprotein E (ApoE) Particles Inhibit Vascular Cell Adhesion Molecule-1 (VCAM-1) Expression in Human Endothelial Cells J. Biol. Chem., November 30, 2001; 276(49): 46011 - 46016. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. J. Blake and P. M. Ridker Novel Clinical Markers of Vascular Wall Inflammation Circ. Res., October 26, 2001; 89(9): 763 - 771. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. C. Barbaux, S. Blankenberg, H. J. Rupprecht, C. Francomme, C. Bickel, G. Hafner, V. Nicaud, J. Meyer, F. Cambien, and L. Tiret Association Between P-Selectin Gene Polymorphisms and Soluble P-Selectin Levels and Their Relation to Coronary Artery Disease Arterioscler Thromb Vasc Biol, October 1, 2001; 21(10): 1668 - 1673. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Kerfoot, E. Raharjo, M. Ho, J. Kaur, S. Serirom, D.-M. McCafferty, A. R. Burns, K. D. Patel, and P. Kubes Exclusive Neutrophil Recruitment with Oncostatin M in a Human System Am. J. Pathol., October 1, 2001; 159(4): 1531 - 1539. [Abstract] [Full Text]
|
|
|
|
|
|
N. Methia, P. Andre, C. V. Denis, M. Economopoulos, and D. D. Wagner Localized reduction of atherosclerosis in von Willebrand factor-deficient mice Blood, September 1, 2001; 98(5): 1424 - 1428. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Skilbeck, S. M. Westwood, P. G. Walker, T. David, and G. B. Nash Population of the Vessel Wall by Leukocytes Binding to P-Selectin in a Model of Disturbed Arterial Flow Arterioscler Thromb Vasc Biol, August 1, 2001; 21(8): 1294 - 1300. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Yoshida, T. Sawada, H. Ishii, R. E. Gerszten, A. Rosenzweig, M. A. Gimbrone Jr, Y. Yasukochi, and F. Numano HMG-CoA Reductase Inhibitor Modulates Monocyte-Endothelial Cell Interaction Under Physiological Flow Conditions In Vitro : Involvement of Rho GTPase-Dependent Mechanism Arterioscler Thromb Vasc Biol, July 1, 2001; 21(7): 1165 - 1171. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. E. ERIKSSON, X. XIE, J. WERR, P. THOREN, and L. LINDBOM Direct viewing of atherosclerosis in vivo: plaque invasion by leukocytes is initiated by the endothelial selectins FASEB J, May 1, 2001; 15(7): 1149 - 1157. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. von Hundelshausen, K. S. C. Weber, Y. Huo, A. E. I. Proudfoot, P. J. Nelson, K. Ley, and C. Weber RANTES Deposition by Platelets Triggers Monocyte Arrest on Inflamed and Atherosclerotic Endothelium Circulation, April 3, 2001; 103(13): 1772 - 1777. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Kubes and S. M. Kerfoot Leukocyte Recruitment in the Microcirculation: the Rolling Paradigm Revisited Physiology, April 1, 2001; 16(2): 76 - 80. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Manka, R. G. Collins, K. Ley, A. L. Beaudet, and I. J. Sarembock Absence of P-Selectin, but Not Intercellular Adhesion Molecule-1, Attenuates Neointimal Growth After Arterial Injury in Apolipoprotein E-Deficient Mice Circulation, February 20, 2001; 103(7): 1000 - 1005. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. S. Pierangeli, R. G. Espinola, X. Liu, and E. N. Harris Thrombogenic Effects of Antiphospholipid Antibodies Are Mediated by Intercellular Cell Adhesion Molecule-1, Vascular Cell Adhesion Molecule-1, and P-Selectin Circ. Res., February 2, 2001; 88(2): 245 - 250. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. M. Ridker, J. E. Buring, and N. Rifai Soluble P-Selectin and the Risk of Future Cardiovascular Events Circulation, January 30, 2001; 103(4): 491 - 495. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-G. Lin, X.-Y. Yu, Y.-X. Chen, X. R. Huang, C. Metz, R. Bucala, C.-P. Lau, and H. Y. Lan De Novo Expression of Macrophage Migration Inhibitory Factor in Atherogenesis in Rabbits Circ. Res., December 8, 2000; 87(12): 1202 - 1208. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. E. Rosenfeld, P. Polinsky, R. Virmani, K. Kauser, G. Rubanyi, and S. M. Schwartz Advanced Atherosclerotic Lesions in the Innominate Artery of the ApoE Knockout Mouse Arterioscler Thromb Vasc Biol, December 1, 2000; 20(12): 2587 - 2592. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Ishii, M. Yoshida, A. Rosenzweig, M. A. Gimbrone Jr, Y. Yasukochi, and F. Numano Adenoviral transduction of human E-selectin into isolated, perfused, rat aortic segments: an ex vivo model for studying leukocyte-endothelial interactions J. Leukoc. Biol., November 1, 2000; 68(5): 687 - 692. [Abstract] [Full Text]
|
|
|
|
|
|
P. Liuba, P. Karnani, E. Pesonen, I. Paakkari, A. Forslid, L. Johansson, K. Persson, T. Wadstrom, and R. Laurini Endothelial Dysfunction After Repeated Chlamydia pneumoniae Infection in Apolipoprotein E-Knockout Mice Circulation, August 29, 2000; 102(9): 1039 - 1044. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Huo, A. Hafezi-Moghadam, and K. Ley Role of Vascular Cell Adhesion Molecule-1 and Fibronectin Connecting Segment-1 in Monocyte Rolling and Adhesion on Early Atherosclerotic Lesions Circ. Res., July 21, 2000; 87(2): 153 - 159. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. E. May, F.-J. Neumann, A. Schomig, and K. T. Preissner VLA-4 (alpha 4beta 1) engagement defines a novel activation pathway for beta 2 integrin-dependent leukocyte adhesion involving the urokinase receptor Blood, July 15, 2000; 96(2): 506 - 513. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Mach Toward New Therapeutic Strategies Against Neointimal Formation in Restenosis Arterioscler Thromb Vasc Biol, July 1, 2000; 20(7): 1699 - 1700. [Full Text] [PDF]
|
|
|
|
|
|
Z. M. Dong, A. A. Brown, and D. D. Wagner Prominent Role of P-Selectin in the Development of Advanced Atherosclerosis in ApoE-Deficient Mice Circulation, May 16, 2000; 101(19): 2290 - 2295. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. G. Collins, R. Velji, N. V. Guevara, M. J. Hicks, L. Chan, and A. L. Beaudet P-Selectin or Intercellular Adhesion Molecule (Icam)-1 Deficiency Substantially Protects against Atherosclerosis in Apolipoprotein E-Deficient Mice J. Exp. Med., January 3, 2000; 191(1): 189 - 194. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Node, Y. Huo, X. Ruan, B. Yang, M. Spiecker, K. Ley, D. C. Zeldin, and J. K. Liao Anti-inflammatory Properties of Cytochrome P450 Epoxygenase-Derived Eicosanoids Science, August 20, 1999; 285(5431): 1276 - 1279. [Abstract] [Full Text]
|
|
|
|
|
|
D. J. Lefer and D. N. Granger Monocyte Rolling in Early Atherogenesis : Vital Role in Lesion Development Circ. Res., June 11, 1999; 84(11): 1353 - 1355. [Full Text] [PDF]
|
|
|
|
|
|
E. Trogan, R. P. Choudhury, H. M. Dansky, J. X. Rong, J. L. Breslow, and E. A. Fisher Laser capture microdissection analysis of gene expression in macrophages from atherosclerotic lesions of apolipoprotein E-deficient mice PNAS, February 19, 2002; 99(4): 2234 - 2239. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Chen, D. Li, T. Saldeen, and J. L. Mehta Transforming Growth Factor-{beta}1 Modulates Oxidatively Modified LDL-Induced Expression of Adhesion Molecules: Role of LOX-1 Circ. Res., December 7, 2001; 89(12): 1155 - 1160. [Abstract] [Full Text] [PDF]
|
|