© 1998 American Heart Association, Inc.
Editorial |
Gap Junction Proteins
Where They Live and How They Die
Correspondence to David C. Spray, PhD, Department of Neuroscience, Room 712 Kennedy Center, Albert Einstein College of Medicine, 1410 Pelham Parkway South, Bronx, NY 10461. E-mail spray@aecom.yu.edu
Key Words: gap junction • connexin • proteolysis • endothelium
Gap junction channels are unique. No other channel in vertebrates provides an enclosed conduit for direct diffusional exchange of ions and small molecules between cells, and few other membrane channels have pore diameters large enough to accommodate passage of metabolites and signaling molecules with molecular weights as high as 1000 Da. Moreover, as addressed in two articles in this issue of Circulation Research, gap junctions are formed by proteins with unusually rapid turnover times1 and extremely flexible expression patterns.2
The connexin proteins that form gap junction channels are encoded by a gene family with at least 14 members in rodents. Each connexin protein has four transmembrane domains, one intracellular and two extracellular loops, and cytoplasmically located carboxyl and amino termini. Six connexin molecules, most likely arranged so that their third transmembrane domains line the channel lumen, comprise the hemichannels or connexons that are contributed by each cell of the coupled pair. Complete gap junction channels, with connexons docked across the gap of extracellular space by interactions of the extracellular loops, are commonly found clustered together, forming islands of particles or pits in freeze-fractured preparations, linearly apposed but slightly separated membranes in thin-section electron micrographs and macular regions of intercellular immunostaining with gap junction antibodies.
Remarkably, in studies first performed on rat liver in
vivo3 4 and subsequently in cardiac myocytes and
hepatocytes and cell lines in
culture,5 6 7 8 the turnover times for connexin
molecules have been found to be very short. The article by Beardslee et
al1 provides direct evidence for
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