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(Circulation Research. 1997;81:86-91.)
© 1997 American Heart Association, Inc.

Articles

Type B Atrial Natriuretic Peptide Receptor in Cardiac Myocyte Caveolae

Donald D. Doyle, S. Kelly Ambler, Judy Upshaw-Earley, Amir Bastawrous, Gwendolyn E. Goings, , Ernest Page

From the Departments of Medicine and Pharmacological and Physiological Sciences, University of Chicago (Ill).

Correspondence to Dr Ernest Page, Department of Medicine (Cardiology), University of Chicago, 5841 S Maryland Ave, MC5085, Chicago, IL 60637. E-mail page{at}hearts.bsd.uchicago.edu

Abstract We have previously shown that atrial natriuretic peptide (ANP) is present in caveolae of in situ rat atrial myocytes. To investigate whether intracaveolar ANP of rat atrial myocytes exists within caveolae bound to type B ANP receptors (ANP-RB, a guanylyl cyclase), we have used confocal immunofluorescence microscopy applied to primary cultures of atrial myocytes from adult rats and to freshly dissociated rat atrial myocytes (not cultured). These experimental designs tested whether atrial myocyte ANP-RB colocalizes at the plasmalemma and elsewhere in the cell with the muscle-specific isoform of the caveolar coating protein, caveolin-3, and with a fraction of cellular ANP. The experiments showed that cellular caveolin-3, a fraction of cellular ANP-RB, and a fraction of cellular ANP colocalize at the plasmalemma of cultured atrial myocytes and of freshly dissociated atrial myocytes. The observations support the hypothesis that in rat atrial myocytes, intracaveolar ANP is bound to ANP-RB, a protein whose cytosolic amino acid sequences are known to encode guanylyl cyclase activity. We suggest that among the (probably multiple) effects of the cGMP thus generated in the cytoplasmic microdomain underlying atrial myocyte caveolae may be the activation of cGMP-dependent protein kinase, which would thereby inhibit plasma membrane Ca²⁺ channel activity and contribute to a negative inotropic effect of ANP.

Key Words: atrial natriuretic peptide B receptor • atrial myocyte • guanylyl cyclase • heart

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