© 1997 American Heart Association, Inc.
Articles |
Epoxyeicosatrienoic Acids Activate K+ Channels in Coronary Smooth Muscle Through a Guanine Nucleotide Binding Protein
From the Department of Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee.
Abstract Epoxyeicosatrienoic acids (EETs) are
endothelium-derived arachidonic acid
metabolites of cytochrome P450. They dilate coronary arteries,
open K+ channels, and hyperpolarize vascular smooth
muscles. However, the mechanisms of these smooth muscle actions remain
unknown. This study examined the effects of EETs on the
large-conductance Ca2+-activated K+
channel (KCa) in smooth muscle cells of small bovine
coronary arteries. In cell-attached patch-clamp experiments,
11,12-EET produced a 0.5- to 10-fold increase in the activity of the
KCa channels when added in concentrations of 1, 10, and 100
nmol/L. In the inside-out excised membrane patch mode, 11,12-EET was
without effect on the activity of the KCa channel unless
GTP (0.5 mmol/L) or GTP and ATP (1 mmol/L) were added to the
bath solution. In the presence of GTP and ATP, the increase in the
KCa channel activity with 11,12-EET in inside-out patches
was comparable to that in cell-attached patches. This effect of
11,12-EET in inside-out patches was blocked by the addition of
GDP-ß-S (100 µmol/L). In outside-out patches, 11,12-EET also
increased the KCa channel activity when GTP and ATP were
added to the pipette solution. The addition of a specific
anti-GS
antibody (100 nmol/L) in the pipette solution
completely blocked the activation of the KCa channels
induced by 11,12-EET. An anti-Gß
or anti-Gi
antibody was without effect. We conclude that 11,12-EET
activates the KCa channels by a
GS-mediated mechanism. This mechanism contributes to the
effects of EETs as endothelium-derived hyperpolarizing
factors to hyperpolarize and relax arterial smooth muscle.
Key Words: endothelium-derived hyperpolarizing factor • patch clamp • K+ channel • eicosanoid • G protein
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
 |
|
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|
|
|
|
|
|
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|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
 |
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|
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|
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P.-L. Li, A.-P. Zou, and W. B. Campbell Regulation of KCa-channel activity by cyclic ADP-ribose and ADP-ribose in coronary arterial smooth muscle Am J Physiol Heart Circ Physiol, September 1, 1998; 275(3): H1002 - H1010. [Abstract] [Full Text] [PDF]
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J. Bylund, T. Kunz, K. Valmsen, and E. H. Oliw Cytochromes P450 with Bisallylic Hydroxylation Activity on Arachidonic and Linoleic Acids Studied with Human Recombinant Enzymes and with Human and Rat Liver Microsomes J. Pharmacol. Exp. Ther., January 1, 1998; 284(1): 51 - 60. [Abstract] [Full Text]
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P.-L. Li, M.-W. Jin, and W. B. Campbell Effect of Selective Inhibition of Soluble Guanylyl Cyclase on the KCa Channel Activity in Coronary Artery Smooth Muscle Hypertension, January 1, 1998; 31(1): 303 - 308. [Abstract] [Full Text] [PDF]
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W. B. Campbell, C. Deeter, K. M. Gauthier, R. H. Ingraham, J. R. Falck, and P.-L. Li 14,15-Dihydroxyeicosatrienoic acid relaxes bovine coronary arteries by activation of KCa channels Am J Physiol Heart Circ Physiol, May 1, 2002; 282(5): H1656 - H1664. [Abstract] [Full Text] [PDF]
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P.-L. Li, D. X. Zhang, Z.-D. Ge, and W. B. Campbell Role of ADP-ribose in 11,12-EET-induced activation of KCa channels in coronary arterial smooth muscle cells Am J Physiol Heart Circ Physiol, April 1, 2002; 282(4): H1229 - H1236. [Abstract] [Full Text] [PDF]
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P. Vequaud and E. Thorin Endothelial G Protein {beta}-Subunits Trigger Nitric Oxide- but not Endothelium-Derived Hyperpolarizing Factor-Dependent Dilation in Rabbit Resistance Arteries Circ. Res., October 12, 2001; 89(8): 716 - 722. [Abstract] [Full Text] [PDF]
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