© 1997 American Heart Association, Inc.
Articles |
In Vitro Modulation of a Resistance Artery Diameter by the Tissue Renin-Angiotensin System of a Large Donor Artery
the Institut National de la Sante et de la Recherche Medicale (INSERM) Unit 141, IFR Circulation Lariboisiere, Universite Paris (France) VII.
Correspondence to D. Henrion, PhD, INSERM U 141, Hopital Lariboisiere, 41 Bd de la Chapelle, 75475 Paris, cedex 10, France. E-mail levy@infobiogen.fr
A local renin-angiotensin system (RAS) is present in the vasculature and might have an important role in the control of vascular resistance. In order to assess its functional role in the control of vasomotor tone, we investigated the effect of the RAS of a donor vessel (rat carotid artery) on the diameter of a recipient rat mesenteric resistance artery. Arteries were perfused in series in an arteriograph at a rate of 100 µL/min, under a pressure of 100 mm Hg. The two vessels were superfused in separate organ chambers to which drugs were added. Recipient artery internal diameter was measured continuously. Phenylephrine (0.1 µmol/L) was present in the organ baths throughout the experiments, ensuring a preconstriction of the recipient artery (236±4 to 174±3 µm, n=65 arterial segments from 34 rats). The angiotensin I–converting enzyme inhibitors (ACEIs) cilazapril (1 µmol/L) and captopril (10 µmol/L) inhibited phenylephrine-induced constriction by 30±12% (n=7, P<.001) and 20±8% (n=5, P<.01), respectively. Addition of cilazapril (1 µmol/L) or captopril (10 µmol/L) to the donor vessel chamber further inhibited the constriction by 8±3% (n=7, P<.01) and 31±10% (n=5, P<.05), respectively. The angiotensin II receptor (AT1) antagonist losartan (10 µmol/L) prevented, in part, the relaxation due to the ACEI. The association of losartan (10 µmol/L) with the bradykinin B2 receptor antagonist HOE 140 (1 µmol/L) totally prevented the relaxation due to the ACEI. Finally, angiotensin II was measured in the perfusate of the carotid artery and was found to be released at a rate of 11.9±2.2 pg in 60 minutes (n=8), which was significantly decreased to 1.4±0.4 pg in 60 minutes (n=4) by cilazapril (1 µmol/L). This study provides functional evidence that tissue-generated angiotensin II and bradykinin, produced locally and in upstream arteries, control the diameter of a resistance mesenteric artery.
Key Words: vascular reactivity • angiotensin II • angiotensin I–converting enzyme inhibitor • captopril • cilazapril
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