This Article Full Text Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Meyer, G. Articles by Tedgui, A. Search for Related Content PubMed PubMed Citation Articles by Meyer, G. Articles by Tedgui, A.

(Circulation Research. 1996;79:532-540.)
© 1996 American Heart Association, Inc.

Articles

Effects of Pressure-Induced Stretch and Convection on Low-Density Lipoprotein and Albumin Uptake in the Rabbit Aortic Wall

Guy Meyer, Regine Merval, Alain Tedgui

INSERM U141 and IFR Circulation Lariboisiere, Hopital Lariboisiere, Paris, France.

Correspondence to Alain Tedgui, INSERM U141, 41 Bd de la Chapelle, 75475 Paris Cedex 10, France.

The effects of pressure-driven convection and vessel wall stretching in the pressure-related changes in low-density lipoprotein (LDL) and albumin transport across the arterial wall were studied in vitro in freshly excised rabbit thoracic aorta held at in vivo length and pressurized at 70, 120, or 160 mm Hg for 30 minutes. External rigid polyester sleeves of various diameters (4, 5, or 6 mm) were passed around half of the arterial segments in order to prevent vessel distension during pressurization. The intraluminal solution contained ¹³¹I-LDL and ¹²⁵I-albumin. The transmural distribution of relative concentrations of LDL (C_LDL) and albumin (C_alb) across the wall was determined in wrapped and unwrapped segments using a serial frozen-sectioning technique. In the unwrapped segments, C_alb increased uniformly between 70 and 120 mm Hg (P<.0001) but did not change significantly between 120 and 160 mm Hg (0.0063±0.0009 [n=4], 0.0520±0.0055 [n=9], and 0.0620±0.0071 [n=12], respectively). In contrast, C_LDL increased markedly both between 70 and 120 mm Hg (P<.001) and between 120 and 160 mm Hg (P<.05) (0.0025±0.0005 [n=4], 0.0234±0.0029 [n=9], and 0.0393±0.0056 [n=12], respectively), with the increase being much more pronounced in the inner than in the outer media. In the segments wrapped with the 4-mm sleeves, both C_LDL and C_alb did not vary significantly between 70, 120, and 160 mm Hg. In the segments wrapped with the 5-mm sleeves, C_LDL increased significantly between 120 and 160 mm Hg, whereas C_alb did not vary significantly with increasing pressure. Our results demonstrate that (1) pressure-induced stretching of the arterial wall is a major determinant of arterial mass transport, and (2) pressure-driven convection accentuates LDL accumulation in the inner media, which may explain enhanced atherosclerosis in hypertension.

Key Words: atherosclerosis • pressure • stretch • convection • LDL transport

This article has been cited by other articles:

				M. Dabagh, P. Jalali, and J. M. Tarbell The transport of LDL across the deformable arterial wall: the effect of endothelial cell turnover and intimal deformation under hypertension Am J Physiol Heart Circ Physiol, September 1, 2009; 297(3): H983 - H996. [Abstract] [Full Text] [PDF]

				U. Olgac, D. Poulikakos, S. C. Saur, H. Alkadhi, and V. Kurtcuoglu Patient-specific three-dimensional simulation of LDL accumulation in a human left coronary artery in its healthy and atherosclerotic states Am J Physiol Heart Circ Physiol, June 1, 2009; 296(6): H1969 - H1982. [Abstract] [Full Text] [PDF]

				K. Lee, G. M. Saidel, and M. S. Penn Permeability change of arterial endothelium is an age-dependent function of lesion size in apolipoprotein E-null mice Am J Physiol Heart Circ Physiol, December 1, 2008; 295(6): H2273 - H2279. [Abstract] [Full Text] [PDF]

				U. Olgac, V. Kurtcuoglu, and D. Poulikakos Computational modeling of coupled blood-wall mass transport of LDL: effects of local wall shear stress Am J Physiol Heart Circ Physiol, February 1, 2008; 294(2): H909 - H919. [Abstract] [Full Text] [PDF]

				J.-B. Michel, O. Thaunat, X. Houard, O. Meilhac, G. Caligiuri, and A. Nicoletti Topological Determinants and Consequences of Adventitial Responses to Arterial Wall Injury Arterioscler Thromb Vasc Biol, June 1, 2007; 27(6): 1259 - 1268. [Abstract] [Full Text] [PDF]

				N. Sun, N. B. Wood, A. D. Hughes, S. A. M. Thom, and X. Yun Xu Effects of transmural pressure and wall shear stress on LDL accumulation in the arterial wall: a numerical study using a multilayered model Am J Physiol Heart Circ Physiol, June 1, 2007; 292(6): H3148 - H3157. [Abstract] [Full Text] [PDF]

				T. Ohtani, Y. Ueda, I. Mizote, J. Oyabu, K. Okada, A. Hirayama, and K. Kodama Number of Yellow Plaques Detected in a Coronary Artery Is Associated With Future Risk of Acute Coronary Syndrome: Detection of Vulnerable Patients by Angioscopy J. Am. Coll. Cardiol., June 6, 2006; 47(11): 2194 - 2200. [Abstract] [Full Text] [PDF]

				A. Tedgui and Z. Mallat Cytokines in Atherosclerosis: Pathogenic and Regulatory Pathways Physiol Rev, April 1, 2006; 86(2): 515 - 581. [Abstract] [Full Text] [PDF]

				A.C. Sposito Emerging insights into hypertension and dyslipidaemia synergies Eur. Heart J. Suppl., December 1, 2004; 6(suppl_G): G8 - G12. [Abstract] [Full Text] [PDF]

				A. Tedgui and Z. Mallat Hypertension: A Novel Regulator of Adaptive Immunity in Atherosclerosis? Hypertension, September 1, 2004; 44(3): 257 - 258. [Full Text] [PDF]

				S. Tada and J. M. Tarbell Internal elastic lamina affects the distribution of macromolecules in the arterial wall: a computational study Am J Physiol Heart Circ Physiol, August 1, 2004; 287(2): H905 - H913. [Abstract] [Full Text] [PDF]

				C. R. Kiefer, J. B. McKenney, J. F. Trainor, and L. M. Snyder Maturation-Dependent Acquired Coronary Structural Alterations and Atherogenesis in the Dahl Sodium-Sensitive Hypertensive Rat Circulation, November 5, 2002; 106(19): 2486 - 2490. [Abstract] [Full Text] [PDF]

				J. O. Toikka, H. Laine, M. Ahotupa, A. Haapanen, J. S. A. Viikari, J. J. Hartiala, and O. T Raitakari Increased Arterial Intima-Media Thickness and In Vivo LDL Oxidation in Young Men With Borderline Hypertension Hypertension, December 1, 2000; 36(6): 929 - 933. [Abstract] [Full Text] [PDF]

				H. Xie, J. A. Bevan, and W. G. Mayhan Oxidized Low-Density Lipoprotein Enhances Myogenic Tone in the Rabbit Posterior Cerebral Artery Through the Release of Endothelin-1 • Editorial Comment Stroke, November 1, 1999; 30 (11): 2423 - 2430. [Abstract] [Full Text] [PDF]

				M. A. Lovich, M. Philbrook, S. Sawyer, E. Weselcouch, and E. R. Edelman Arterial heparin deposition: role of diffusion, convection, and extravascular space Am J Physiol Heart Circ Physiol, December 1, 1998; 275(6): H2236 - H2242. [Abstract] [Full Text] [PDF]

				N. Inoue, S. Kawashima, K.-I. Hirata, Y. Rikitake, S. Takeshita, W. Yamochi, H. Akita, and M. Yokoyama Stretch force on vascular smooth muscle cells enhances oxidation of LDL via superoxide production Am J Physiol Heart Circ Physiol, June 1, 1998; 274(6): H1928 - H1932. [Abstract] [Full Text] [PDF]