This Article Full Text Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Tepel, M. Articles by Zidek, W. Search for Related Content PubMed PubMed Citation Articles by Tepel, M. Articles by Zidek, W. Pubmed/NCBI databases Compound via MeSH Substance via MeSH Hazardous Substances DB SODIUM

(Circulation Research. 1995;77:1024.)
© 1995 American Heart Association, Inc.

Articles

Lymphocytic Na⁺-H⁺ Exchange Increases After an Oral Glucose Challenge

Martin Tepel, Ralf Schlotmann, Michael Barenbrock, Klaus Kisters, Torsten Klaus, Claus Spieker, Michael Walter, Christian Meyer, Reinhard G. Bretzel, Walter Zidek

From Medizinische Universitäts-Poliklinik (M.T., R.S., M.B., K.K., T.K., C.S., W.Z.) and Institut für Klinische Chemie und Laboratoriumsmedizin (M.W.), University of Münster (Germany), and Medizinische Klinik und Poliklinik (C.M., R.G.B.), University of Giessen (Germany).

Correspondence to Prof W. Zidek, Med. Univ.-Poliklinik, Albert-Schweitzer-Str. 33, D-48129 Münster, Germany.

Abstract The effects of oral glucose challenge on plasma glucose concentration, plasma insulin concentration, arterial blood pressure, cytosolic pH (pH_i), cytosolic free Na⁺ concentration ([Na⁺]_i), and cellular Na⁺-H⁺ exchange activity were investigated in 16 healthy subjects. The pH_i, [Na⁺]_i, and Na⁺-H⁺ exchange activity were measured in intact lymphocytes by using the fluorescent dye technique. The oral glucose challenge significantly increased plasma glucose, plasma insulin, and the lymphocytic Na⁺-H⁺ exchange activity, measured as change of pH_i per second (control [0 hours], 5.20±0.53x10^-3 dpH_i/s; 1 hour after glucose administration, 8.28±1.07x10^-3 dpH_i/s; 2 hours after glucose administration, 8.15±1.18x10^-3 dpH_i/s; P=.002). The lymphocytic Na⁺-H⁺ exchange was significantly correlated with plasma glucose concentration (r=.357, P=.041). During steady state euglycemic hyperinsulinemic clamp, the Na⁺-H⁺ exchange activity was not significantly changed compared with baseline values. The study shows that changes of blood glucose levels can induce an acute increase in Na⁺-H⁺ exchange activity. Systolic blood pressure and Na⁺-H⁺ exchange activity were significantly (P<.001) but weakly correlated during an oral glucose challenge.

Key Words: Na⁺-H⁺ exchange • glucose • insulin

This article has been cited by other articles:

				V. Jankowski, R. Vanholder, M. van der Giet, M. Tolle, S. Karadogan, J. Gobom, J. Furkert, A. Oksche, E. Krause, T. N. Anh Tran, et al. Mass-Spectrometric Identification of a Novel Angiotensin Peptide in Human Plasma Arterioscler Thromb Vasc Biol, February 1, 2007; 27(2): 297 - 302. [Abstract] [Full Text] [PDF]

				J.-R. Nofer, M. Tepel, M. Walter, U. Seedorf, G. Assmann, and W. Zidek Phosphatidylcholine-specific Phospholipase C Regulates Thapsigargin-induced Calcium Influx in Human Lymphocytes J. Biol. Chem., December 26, 1997; 272(52): 32861 - 32868. [Abstract] [Full Text] [PDF]

				J.-R. Nofer, M. Tepel, B. Kehrel, S. Wierwille, M. Walter, U. Seedorf, W. Zidek, and G. Assmann Low-Density Lipoproteins Inhibit the Na+/H+ Antiport in Human Platelets: A Novel Mechanism Enhancing Platelet Activity in Hypercholesterolemia Circulation, March 18, 1997; 95(6): 1370 - 1377. [Abstract] [Full Text]