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(Circulation Research. 1995;77:342-353.)
© 1995 American Heart Association, Inc.

Articles

Differential Phospholamban Gene Expression in Murine Cardiac Compartments

Molecular and Physiological Analyses

Kimberly L. Koss, Sathivel Ponniah, W. Keith Jones, Ingrid L. Grupp, Evangelia G. Kranias

From the Departments of Molecular and Cellular Physiology (K.L.K.) and Pharmacology and Cell Biophysics (S.P., I.L.G., E.G.K.) and the Division of Cardiovascular Biology (Children's Medical Center) (W.K.J.), University of Cincinnati (Ohio) College of Medicine.

Correspondence to Evangelia G. Kranias, Department of Pharmacology and Cell Biophysics, University of Cincinnati College of Medicine, 231 Bethesda Ave, Cincinnati, OH 45267-0575.

Abstract Phospholamban, the regulator of the Ca²⁺ pump in cardiac sarcoplasmic reticulum, is differentially expressed between murine atrial and ventricular muscles. Quantitative analyses of RNA isolated from atrial flaps and ventricular apices indicated that the phospholamban gene transcript copy number is 2.5-fold higher in the ventricle compared with the atrium of the FVB/N mouse and 6-fold higher in the ventricle compared with the atrium of the B6D2/F1 mouse strain. These findings were corroborated by in situ hybridization studies of cardiopulmonary sections from both murine strains, and phospholamban transcripts were also observed in pulmonary myocardia of both strains. Analyses of phospholamban transcript levels relative to -myosin heavy chain (-MHC) revealed a 3-fold higher phospholamban abundance in the ventricle compared with the atrium of the FVB/N murine strain. However, the relative mRNA level of Ca²⁺-ATPase (ratio of sarcoplasmic reticulum Ca²⁺-ATPase [SERCA2] to -MHC) in the ventricle was 80% of that in the atrium. Consequently, the relative ratio of phospholamban to SERCA2 mRNA was 4.2-fold lower in the atrium than in the ventricle. The lower transcript ratio of phospholamban to SERCA2 in the atrium was associated with significantly shortened times to half-relaxation (17.40±0.71 milliseconds for atrium versus 30.58±2.04 milliseconds for ventricle), assessed in isolated superfused cardiac tissue preparations recorded at maximum length tension. Contraction times, measured as times to peak tension, were also significantly shortened in atrial muscle (27.36±0.82 milliseconds) compared with ventricular muscle (44.60±2.55 milliseconds), assessed in the same tissue preparations. These findings suggest that phospholamban gene expression is differentially regulated in murine atrial and ventricular muscles and that this differential expression may be associated with differences in the contractile parameters of these cardiac compartments.

Key Words: phospholamban • atrium • ventricle • contractility • sarcoplasmic reticulum Ca²⁺-ATPase

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