© 1995 American Heart Association, Inc.
Articles |
Postischemic Changes in Cardiac Sarcoplasmic Reticulum Ca2+ Channels
A Possible Mechanism of Ischemic Preconditioning
From Scuola Superiore S. Anna (R.Z.), Istituto di Cardiologia (R.Z., M.M.), and Istituto di Chimica Biologica (S.R.-T., G.Y., P.G., G.R.), University of Pisa (Italy).
Correspondence to R. Zucchi, MD, Scuola Superiore S. Anna, via Carducci 40, I-56100 Pisa, Italy.
Abstract We investigated the modifications of cardiac ryanodine receptors/sarcoplasmic reticulum Ca2+ release channels occurring in ischemic preconditioning. In an isolated rat heart model, the injury produced by 30 minutes of global ischemia was reduced by preexposure to three 3-minute periods of global ischemia (preconditioning ischemia). The protection was still present 120 minutes after preconditioning ischemia but disappeared after 240 minutes. Three 1-minute periods of global ischemia did not provide any protection. In the crude homogenate obtained from ventricular myocardium, the density of [3H]ryanodine binding sites averaged 372±18 fmol/mg of protein in the control condition, decreased 5 minutes after preconditioning ischemia (290±15 fmol/mg, P<.01), was still significantly reduced after 120 minutes (298±17 fmol/mg, P<.05), and recovered after 240 minutes (341±21 fmol/mg). Three 1-minute periods of ischemia did not produce any change in ryanodine binding. The Kd for ryanodine (1.5±0.3 nmol/L) was unchanged in all cases. In parallel experiments, the crude homogenate or a microsomal fraction was passively loaded with 45Ca, and Ca2+-induced Ca2+ release was studied by the quick filtration technique. In both preparations, the rate constant of Ca2+-induced Ca2+ release decreased 5 and 120 minutes after preconditioning ischemia (homogenate values: 19.7±1.4 and 18.9±0.9 s-1 vs a control value of 25.4±1.7 s-1, P<.05 in both cases) and recovered after 240 minutes (23.0±1.9 s-1). The Ca2+ dependence of Ca2+-induced Ca2+ release was not affected by preconditioning ischemia. In conclusion, changes in sarcoplasmic reticulum Ca2+-release channels occur after brief ischemia and reperfusion, are closely correlated with the development of myocardial protection versus sustained ischemia, and might play a role in the pathogenesis of ischemic preconditioning.
Key Words: sarcoplasmic reticulum • Ca2+ • ischemia • reperfusion
This article has been cited by other articles:
 |
|
 |
|
  C. Wang, J.-F. Du, F. Wu, and H.-C. Wang Apelin decreases the SR Ca2+ content but enhances the amplitude of [Ca2+]i transient and contractions during twitches in isolated rat cardiac myocytes Am J Physiol Heart Circ Physiol, June 1, 2008; 294(6): H2540 - H2546. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Yamamura, C. Steenbergen, and E. Murphy Protein kinase C and preconditioning: role of the sarcoplasmic reticulum Am J Physiol Heart Circ Physiol, December 1, 2005; 289(6): H2484 - H2490. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Dworschak, L. V. d'Uscio, D. Breukelmann, and J. D. Hannon Increased tolerance to hypoxic metabolic inhibition and reoxygenation of cardiomyocytes from apolipoprotein E-deficient mice Am J Physiol Heart Circ Physiol, July 1, 2005; 289(1): H160 - H167. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Singh and H. K. Saini Resident Cardiac Mast Cells and Ischemia-Reperfusion Injury Journal of Cardiovascular Pharmacology and Therapeutics, June 1, 2003; 8(2): 135 - 148. [Abstract] [PDF]
|
|
|
|
|
|
R. M. Temsah, K. Kawabata, D. Chapman, and N. S. Dhalla Preconditioning prevents alterations in cardiac SR gene expression due to ischemia-reperfusion Am J Physiol Heart Circ Physiol, April 1, 2002; 282(4): H1461 - H1466. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Mubagwa Does adenosine protect the heart by acting on the sarcoplasmic reticulum? Cardiovasc Res, February 1, 2002; 53(2): 286 - 289. [Full Text] [PDF]
|
|
|
|
|
|
R. Zucchi, R. J Cerniway, S. Ronca-Testoni, R.R. Morrison, G. Ronca, and G.P. Matherne Effect of cardiac A1 adenosine receptor overexpression on sarcoplasmic reticulum function Cardiovasc Res, February 1, 2002; 53(2): 326 - 333. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. An, S. G. Varadarajan, E. Novalija, and D. F. Stowe Ischemic and anesthetic preconditioning reduces cytosolic [Ca2+] and improves Ca2+ responses in intact hearts Am J Physiol Heart Circ Physiol, October 1, 2001; 281(4): H1508 - H1523. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Mubagwa and W. Flameng Adenosine, adenosine receptors and myocardial protection: An updated overview Cardiovasc Res, October 1, 2001; 52(1): 25 - 39. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Zucchi, G. Yu, S. Ghelardoni, F. Ronca, and S. Ronca-Testoni A3 adenosine receptor stimulation modulates sarcoplasmic reticulum Ca2+ release in rat heart Cardiovasc Res, April 1, 2001; 50(1): 56 - 64. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Tani, Y. Honma, H. Hasegawa, and K. Tamaki Direct activation of mitochondrial KATP channels mimics preconditioning but protein kinase C activation is less effective in middle-aged rat hearts Cardiovasc Res, January 1, 2001; 49(1): 56 - 68. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Osada, T. Netticadan, K. Kawabata, K. Tamura, and N. S. Dhalla Ischemic preconditioning prevents I/R-induced alterations in SR calcium-calmodulin protein kinase II Am J Physiol Heart Circ Physiol, June 1, 2000; 278(6): H1791 - H1798. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K.-I. Kawabata, T. Netticadan, M. Osada, K. Tamura, and N. S. Dhalla Mechanisms of ischemic preconditioning effects on Ca2+ paradox-induced changes in heart Am J Physiol Heart Circ Physiol, March 1, 2000; 278(3): H1008 - H1015. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Ohkusa, T. Ueyama, J. Yamada, M. Yano, Y. Fujumura, K. Esato, and M. Matsuzaki Alterations in cardiac sarcoplasmic reticulum Ca2+ regulatory proteins in the atrial tissue of patients with chronic atrial fibrillation J. Am. Coll. Cardiol., July 1, 1999; 34(1): 255 - 263. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Hittinger, B. Ghaleh, J. Chen, J. G. Edwards, R. K. Kudej, M. Iwase, S.-J. Kim, S. F. Vatner, and D. E. Vatner Reduced Subendocardial Ryanodine Receptors and Consequent Effects on Cardiac Function in Conscious Dogs With Left Ventricular Hypertrophy Circ. Res., May 14, 1999; 84(9): 999 - 1006. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Zucchi, G. Yu, P. Galbani, M. Mariani, G. Ronca, and S. Ronca-Testoni Sulfhydryl Redox State Affects Susceptibility to Ischemia and Sarcoplasmic Reticulum Ca2+ Release in Rat Heart : Implications for Ischemic Preconditioning Circ. Res., November 2, 1998; 83(9): 908 - 915. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Ueyama, T. Ohkusa, M. Yano, and M. Matsuzaki Growth hormone preserves cardiac sarcoplasmic reticulum Ca2+ release channels (ryanodine receptors) and enhances cardiac function in cardiomyopathic hamsters Cardiovasc Res, October 1, 1998; 40(1): 64 - 73. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Osada, T. Netticadan, K. Tamura, and N. S. Dhalla Modification of ischemia-reperfusion-induced changes in cardiac sarcoplasmic reticulum by preconditioning Am J Physiol Heart Circ Physiol, June 1, 1998; 274(6): H2025 - H2034. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. V. Ladilov, C. Balser, and H. M. Piper Protection of Rat Cardiomyocytes Against Simulated Ischemia and Reoxygenation by Treatment With Protein Kinase C Activator Circ. Res., March 9, 1998; 82(4): 451 - 457. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. R. Meldrum Tumor necrosis factor in the heart Am J Physiol Regulatory Integrative Comp Physiol, March 1, 1998; 274(3): R577 - R595. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Ueyama, T. Ohkusa, Y. Hisamatsu, Y. Nakamura, T. Yamamoto, M. Yano, and M. Matsuzaki Alterations in cardiac SR Ca2+-release channels during development of heart failure in cardiomyopathic hamsters Am J Physiol Heart Circ Physiol, January 1, 1998; 274(1): H1 - H7. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Zhu and G. R. Ferrier Ischemic preconditioning: antiarrhythmic effects and electrophysiological mechanisms in isolated ventricle Am J Physiol Heart Circ Physiol, January 1, 1998; 274(1): H66 - H75. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Przyklenk, K. Hata, and R. A. Kloner Is Calcium a Mediator of Infarct Size Reduction With Preconditioning in Canine Myocardium? Circulation, August 19, 1997; 96(4): 1305 - 1312. [Abstract] [Full Text]
|
|
|
|
|
|
M. Tani, Y. Suganuma, H. Hasegawa, K. Shinmura, Y. Hayashi, X.-d. Guo, and Y. Nakamura Changes in Ischemic Tolerance and Effects of Ischemic Preconditioning in Middle-aged Rat Hearts Circulation, June 3, 1997; 95(11): 2559 - 2566. [Abstract] [Full Text]
|
|
|
|
 |
|
 R. Zucchi and S. Ronca-Testoni The Sarcoplasmic Reticulum Ca2+ Channel/Ryanodine Receptor: Modulation by Endogenous Effectors, Drugs and Disease States Pharmacol. Rev., March 1, 1997; 49(1): 1 - 52. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Menasche, C. Mouas, and C. Grousset Is Potassium Channel Opening an Effective Form of Preconditioning Before Cardioplegia? Ann. Thorac. Surg., June 1, 1996; 61(6): 1764 - 1768. [Abstract] [Full Text]
|
|