This Article Full Text Full Text (PDF) All Versions of this Article: 100/7/1000    most recent 01.RES.0000262688.14288.b8v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Rana, M. S. Articles by van den Hoff, M. J.B. Search for Related Content PubMed PubMed Citation Articles by Rana, M. S. Articles by van den Hoff, M. J.B. Related Collections Cardiac development Related Article

(Circulation Research. 2007;100:1000.)
© 2007 American Heart Association, Inc.

Molecular Medicine

Trabeculated Right Ventricular Free Wall in the Chicken Heart Forms by Ventricularization of the Myocardium Initially Forming the Outflow Tract

M. Sameer Rana, Noortje C.A. Horsten, Sabina Tesink-Taekema, Wout H. Lamers, Antoon F.M. Moorman, Maurice J.B. van den Hoff

From the Heart Failure Research Center, Academic Medical Center (M.S.R., N.C.A.H., S.T.-T., W.H.L., A.F.M.M., M.J.B.v.d.H.), University of Amsterdam, The Netherlands.

Correspondence to Maurice J.B. van den Hoff, Academic Medical Center, Department of Anatomy and Embryology, Meibergdreef 15, 1105 AZ Amsterdam, The Netherlands. E-mail m.j.vandenhoff{at}amc.uva.nl

Recent molecular lineage analyses in mouse have demonstrated that the right ventricle is recruited from anterior mesoderm in later stages of cardiac development. This is in contrast to current views of development in the chicken heart, which suggest that the initial heart tube contains a subset of right ventricular precursors. We investigated the fate of the outflow tract myocardium using immunofluorescent staining of the myocardium, and lineage tracer, as well as cell death experiments. These analyses showed that the outflow tract is initially myocardial in its entirety, increasing in length up to HH24. The outflow tract myocardium, subsequently, shortens as a result of ventricularization, contributing to the trabeculated free wall, as well as the infundibulum, of the right ventricle. During this shortening, the overall length of the outflow tract is maintained because of the formation of a nonmyocardial portion between the distal myocardial border and the pericardial reflections. Cell death and transdifferentiation were found to play a more limited contribution to the initial shortening than is generally appreciated, if they play any part at all. Cell death, nonetheless, plays an important role in the disappearance of the myocardial collar that continues to invest the aorta and pulmonary trunk around HH30, and in the separation of the intrapericardial arterial vessels. Taken together, we show, as opposed to some current beliefs, the development of the arterial pole is similar in mammals and birds.

Key Words: cardiac development • outflow tract • differentiation • cell death

Related Article:

Building the Right Ventricle

Robert G. Kelly
Circ. Res. 2007 100: 943-945. [Full Text] [PDF]

This article has been cited by other articles:

				H. Liu and S. A. Fisher Hypoxia-Inducible Transcription Factor-1{alpha} Triggers an Autocrine Survival Pathway During Embryonic Cardiac Outflow Tract Remodeling Circ. Res., June 6, 2008; 102(11): 1331 - 1339. [Abstract] [Full Text] [PDF]