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(Circulation Research. 2007;100:363.)
© 2007 American Heart Association, Inc.

Molecular Medicine

Notch Ligand Delta-Like 1 Is Essential for Postnatal Arteriogenesis

Anne Limbourg^*, Merlin Ploom^*, Diana Elligsen, Inga Sörensen, Tibor Ziegelhoeffer, Achim Gossler, Helmut Drexler, Florian P. Limbourg

From the Department of Cardiology (A.L., M.P., D.E., I.S., H.D., F.P.L.) and Institute for Molecular Biology (A.G.), Medizinische Hochschule Hannover, Hannover, Germany; and the Department of Thoracic and Cardiothoracic Surgery (T.Z.), Kerckhoff Heart Center, Bad Nauheim, Germany.

Correspondence to Dr. med. Florian P. Limbourg, Kardiologie und Angiologie, OE 6880, Med. Hochschule Hannover, Carl-Neubergstr. 1, 30625 Hannover, Germany. E-mail limbourg.florian{at}mh-hannover.de

Growth of functional arteries is essential for the restoration of blood flow to ischemic organs. Notch signaling regulates arterial differentiation upstream of ephrin-B2 during embryonic development, but its role during postnatal arteriogenesis is unknown. Here, we identify the Notch ligand Delta-like 1 (Dll1) as an essential regulator of postnatal arteriogenesis. Dll1 expression was specifically detected in arterial endothelial cells, but not in venous endothelial cells or capillaries. During ischemia-induced arteriogenesis endothelial Dll1 expression was strongly induced, Notch signaling activated and ephrin-B2 upregulated, whereas perivascular cells expressed proangiogenic vascular endothelial growth factor, and the ephrin-B2 activator EphB4. In heterozygous Dll1 mutant mice endothelial Notch activation and ephrin-B2 induction after hindlimb ischemia were absent, arterial collateral growth was abrogated and recovery of blood flow was severely impaired, but perivascular vascular endothelial growth factor and EphB4 expression was unaltered. In vitro, angiogenic growth factors synergistically activated Notch signaling by induction of Dll1, which was necessary and sufficient to regulate ephrin-B2 expression and to induce ephrin-B2 and EphB4-dependent branching morphogenesis in human arterial EC. Thus, Dll1-mediated Notch activation regulates ephrin-B2 expression and postnatal arteriogenesis.

Key Words: arteriogenesis • vascular biology • endothelium • ischemia

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