Published online before print August 10, 2006, doi: 10.1161/01.RES.0000240500.96746.ec
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Submitted on December 14, 2005
Revised on July 6, 2006
Accepted on August 1, 2006
Activation of D3 Dopamine Receptor Decreases Angiotensin II Type 1 Receptor Expression in Rat Renal Proximal Tubule Cells
Chunyu Zeng *;
From the Department of Cardiology (C.Z., Y.L., D.H.), Daping Hospital, and Xinqiao Hospital (L.H.), Third Military Medical University, Chongqing, People’s Republic of China; Departments of Pediatrics (Z.W., P.Y., S.Z., J.E.J., L.D.A., G.M.E., P.A.J.), Physiology and Biophysics (P.A.J.), and Internal Medicine (G.M.E.), Georgetown University Medical Center, Wash DC; Department of Physiology and Biophysics (U.H.), Case Western Reserve School of Medicine, Cleveland, Ohio; and Department of Pathology (R.A.F.), Virginia University for the Health Sciences, Charlottesville.
* To whom correspondence should be addressed. E-mail: cyzeng1{at}hotmail.com.
The dopaminergic and renin angiotensin systems interact to regulate blood pressure. Disruption of the D3 dopamine receptor gene in mice produces renin-dependent hypertension. In rats, D2-like receptors reduce angiotensin II binding sites in renal proximal tubules (RPTs). Because the major D2-like receptor in RPTs is the D3 receptor, we examined whether D3 receptors regulate angiotensin II type 1 (AT1) receptors in rat RPT cells. The effect of D3 receptors on AT1 receptors was studied in vitro and in vivo. The D3 receptor agonist PD128907 decreased AT1 receptor protein and mRNA in WKY RPT cells and increased it in SHR cells. PD128907 increased D3 receptors in WKY cells but had no effect in SHR cells. D3/AT1 receptors colocalized in RPT cells; D3 receptor stimulation decreased the percent amount of D3 receptors that coimmunoprecipitated with AT1 receptors to a greater extent in WKY than in SHR cells. However, D3 receptor stimulation did not change the percent amount of AT1 receptors that coimmunoprecipitated with D3 receptors in WKY cells; markedly decreased the coimmunoprecipitation in SHR cells. The D3 receptor also regulated the AT1 receptor in vivo because AT1 receptor expression was increased in kidneys of D3 receptor-null mice compared with wild type littermates. D3 receptors may regulate AT1 receptor function by direct interaction with and regulation of AT1 receptor expression. One mechanism of hypertension may be related to increased renal expression of AT1 receptors due decreased D3 receptor regulation.
Key words: AT1 receptor • D3 receptor • renal proximal tubule cells • hypertension
This article has been cited by other articles:
 |
|
 |
|
  V. A. M. Villar, J. E. Jones, I. Armando, C. Palmes-Saloma, P. Yu, A. M. Pascua, L. Keever, F. B. Arnaldo, Z. Wang, Y. Luo, et al. G Protein-coupled Receptor Kinase 4 (GRK4) Regulates the Phosphorylation and Function of the Dopamine D3 Receptor J. Biol. Chem., August 7, 2009; 284(32): 21425 - 21434. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. L. Badole, N. M. Patel, P. A. Thakurdesai, and S. L. Bodhankar Interaction of Aqueous Extract of Pleurotus pulmonarius (Fr.) Quel-Champ. with Glyburide in Alloxan Induced Diabetic Mice Evid. Based Complement. Altern. Med., June 1, 2008; 5(2): 159 - 164. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Li, C. Yu, Y. Han, H. Ren, W. Shi, C. Fu, D. He, L. Huang, C. Yang, X. Wang, et al. Inhibitory effect of D1-like and D3 dopamine receptors on norepinephrine-induced proliferation in vascular smooth muscle cells Am J Physiol Heart Circ Physiol, June 1, 2008; 294(6): H2761 - H2768. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Zeng, I. Armando, Y. Luo, G. M. Eisner, R. A. Felder, and P. A. Jose Dysregulation of dopamine-dependent mechanisms as a determinant of hypertension: studies in dopamine receptor knockout mice Am J Physiol Heart Circ Physiol, February 1, 2008; 294(2): H551 - H569. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Mogi, M. Iwai, and M. Horiuchi Emerging Concepts of Regulation of Angiotensin II Receptors: New Players and Targets for Traditional Receptors Arterioscler Thromb Vasc Biol, December 1, 2007; 27(12): 2532 - 2539. [Abstract] [Full Text] [PDF]
|
|